{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T06:09:36Z","timestamp":1760249376927,"version":"build-2065373602"},"reference-count":68,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2016,9,8]],"date-time":"2016-09-08T00:00:00Z","timestamp":1473292800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"publisher","award":["349-2006-267","621-2011-5848"],"award-info":[{"award-number":["349-2006-267","621-2011-5848"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Swedish Strategic Research Foundation","award":["A306:218"],"award-info":[{"award-number":["A306:218"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>There is a need to identify novel scaffolds and targets to develop new antibiotics. Methylene blue is a phenothiazine derivative, and it has been shown to possess anti-malarial and anti-trypanosomal activities. Here, we show that different phenothiazine derivatives and pyronine G inhibited the activities of three structurally different bacterial RNase P RNAs (RPRs), including that from Mycobacterium tuberculosis, with Ki values in the lower \u03bcM range. Interestingly, three antipsychotic phenothiazines (chlorpromazine, thioridazine, and trifluoperazine), which are known to have antibacterial activities, also inhibited the activity of bacterial RPRs, albeit with higher Ki values than methylene blue. Phenothiazines also affected lead(II)-induced cleavage of bacterial RPR and inhibited yeast tRNAPhe, indicating binding of these drugs to functionally important regions. Collectively, our findings provide the first experimental data showing that long, noncoding RNAs could be targeted by different phenothiazine derivatives.<\/jats:p>","DOI":"10.3390\/biom6030038","type":"journal-article","created":{"date-parts":[[2016,9,8]],"date-time":"2016-09-08T10:08:36Z","timestamp":1473329316000},"page":"38","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Inhibition of Bacterial RNase P RNA by Phenothiazine Derivatives"],"prefix":"10.3390","volume":"6","author":[{"given":"Shiying","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Cell and Molecular Biology, Box 596, Biomedical Centre, Uppsala SE-751 24, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guanzhong","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, Box 596, Biomedical Centre, Uppsala SE-751 24, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leif","family":"Kirsebom","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, Box 596, Biomedical Centre, Uppsala SE-751 24, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,9,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1002\/iub.160","article-title":"RNase P RNA-mediated cleavage","volume":"61","author":"Kirsebom","year":"2009","journal-title":"IUBMB Life"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Liu, F., and Altman, S. 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