{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T20:07:52Z","timestamp":1775160472178,"version":"3.50.1"},"reference-count":38,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T00:00:00Z","timestamp":1537315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010198","name":"Ministerio de Econom\u00eda, Industria y Competitividad, Gobierno de Espa\u00f1a","doi-asserted-by":"publisher","award":["MAT2017-84277-R"],"award-info":[{"award-number":["MAT2017-84277-R"]}],"id":[{"id":"10.13039\/501100010198","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Universidad Europea de Madrid","award":["2017\/UEM21"],"award-info":[{"award-number":["2017\/UEM21"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>The aim of this work is to study, in an in vitro head and neck squamous cell carcinomas model the anti-angiogenic and anti-migratory properties of self-assembled polymeric nanoparticles (NPs) with demonstrated selective anticancer activity. The NPs are based on \u03b1-tocopheryl succinate (\u03b1-TOS) encapsulated in the hydrophobic core of the NPs. We analyzed the effect of the newly synthetized \u03b1-TOS-loaded NPs in proliferating endothelial cells and hypopharynx carcinoma squamous cells and measured markers of angiogenesis, apoptosis and reactive oxygen species (ROS). \u03b1-TOS-loaded NPs suppressed angiogenesis by inducing accumulation of ROS and inducing apoptosis of proliferating endothelial cells. These NPs also decrease the number and quality of capillary-like tubes in an in vitro three-dimensional (3D) experiment, decrease the production of the pro-angiogenic vascular endothelial growth factor and down-regulate the expression of its receptor. The anti-migratory efficacy of \u03b1-TOS is corroborated in hypopharynx carcinoma cells by decreasing the secretion of matrix metalloproteases 2 and 9 (MMP-2 and MMP-9) and inhibiting cell migration. These results confirm that \u03b1-TOS-based NPs not only present anticancer properties, but also antiangiogenic properties, therefore making them promising candidates for multi-active combinatorial anticancer therapy.<\/jats:p>","DOI":"10.3390\/biom8030097","type":"journal-article","created":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T10:50:31Z","timestamp":1537354231000},"page":"97","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["\u03b1-Tocopheryl Succinate-Based Polymeric Nanoparticles for the Treatment of Head and Neck Squamous Cell Carcinoma"],"prefix":"10.3390","volume":"8","author":[{"given":"Carolina","family":"S\u00e1nchez-Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, Calle del Tajo S\/N, Villaviciosa de Od\u00f3n, 28670 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raquel","family":"Palao-Suay","sequence":"additional","affiliation":[{"name":"Group of Biomaterials, Department of Polymeric Nanomaterials and Biomaterials, Institute of Polymer Science and Technology, ICTP-CSIC, C\/Juan de la Cierva, 3, 28006 Madrid, Spain"},{"name":"Networking Biomedical Research Centre in Bioengineering, Biomaterials, and Nanomedicine, CIBER-BBN, Av. Monforte de Lemos, 3-5, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Rodrig\u00e1\u00f1ez","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Hospital Universitario de Getafe, Carretera de Toledo, km 12,500, 28905 Getafe (Madrid), Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7395-5754","authenticated-orcid":false,"given":"Mar\u00eda Rosa","family":"Aguilar","sequence":"additional","affiliation":[{"name":"Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, Calle del Tajo S\/N, Villaviciosa de Od\u00f3n, 28670 Madrid, Spain"},{"name":"Group of Biomaterials, Department of Polymeric Nanomaterials and Biomaterials, Institute of Polymer Science and Technology, ICTP-CSIC, C\/Juan de la Cierva, 3, 28006 Madrid, Spain"},{"name":"Networking Biomedical Research Centre in Bioengineering, Biomaterials, and Nanomedicine, CIBER-BBN, Av. Monforte de Lemos, 3-5, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9506-8483","authenticated-orcid":false,"given":"Sergio","family":"Mart\u00edn-Salda\u00f1a","sequence":"additional","affiliation":[{"name":"Group of Biomaterials, Department of Polymeric Nanomaterials and Biomaterials, Institute of Polymer Science and Technology, ICTP-CSIC, C\/Juan de la Cierva, 3, 28006 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio","family":"San Rom\u00e1n","sequence":"additional","affiliation":[{"name":"Group of Biomaterials, Department of Polymeric Nanomaterials and Biomaterials, Institute of Polymer Science and Technology, ICTP-CSIC, C\/Juan de la Cierva, 3, 28006 Madrid, Spain"},{"name":"Networking Biomedical Research Centre in Bioengineering, Biomaterials, and Nanomedicine, CIBER-BBN, Av. Monforte de Lemos, 3-5, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Sanz-Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, Calle del Tajo S\/N, Villaviciosa de Od\u00f3n, 28670 Madrid, Spain"},{"name":"Department of Otolaryngology, Hospital Universitario de Getafe, Carretera de Toledo, km 12,500, 28905 Getafe (Madrid), Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,9,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1001\/archotol.131.3.204","article-title":"The effect of tetrathiomolybdate on cytokine expression, angiogenesis, and tumor growth in squamous cell carcinoma of the head and neck","volume":"131","author":"Teknos","year":"2005","journal-title":"Arch. Otolaryngol. 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