{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:42:08Z","timestamp":1792035728656,"version":"4.4.0"},"reference-count":52,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2019,5,8]],"date-time":"2019-05-08T00:00:00Z","timestamp":1557273600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"This work was supported by the NSFC (61703152), Hunan Provincial Natural Science Foundation (2016JJ4010, 2018JJ3134), Postgraduates Innovation Fund of School of Life Science and Chemistry in HUT, Doctoral Program Construction of Hunan University of Techno","award":["This work was supported by the NSFC (61703152), Hunan Provincial Natural Science Foundation (2016JJ4010, 2018JJ3134), Postgraduates Innovation Fund of School of Life Science and Chemistry in HUT, Doctoral Program Construction of Hunan University of Techno"],"award-info":[{"award-number":["This work was supported by the NSFC (61703152), Hunan Provincial Natural Science Foundation (2016JJ4010, 2018JJ3134), Postgraduates Innovation Fund of School of Life Science and Chemistry in HUT, Doctoral Program Construction of Hunan University of Techno"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>In this paper, a nanocomposite of cuprous oxide and electrochemically reduced graphene oxide (Cu2O\u2013ERGO) was prepared by a simple and low-cost method; hereby, a new method for the electrochemical determination of tryptophan (Trp) by this composite modified glassy carbon electrode (GCE) is proposed. The prepared materials and modified electrodes were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and cyclic voltammetry (CV). The results showed that Cu2O\u2013ERGO\/GCE had good electrocatalytic activity for Trp. The effects of supporting electrolyte, scanning rate, accumulation potential, and accumulation time on the determination of Trp were studied. Under the optimum experimental conditions, Trp was quantitatively analyzed by square-wave voltammetry (SWV). The oxidation peak current of Trp had a good linear relationship with its concentration in the range of 0.02\u201320 \u03bcM, and the detection limit was 0.01 \u03bcM (S\/N = 3). In addition, the modified electrode has high sensitivity, good repeatability, and long-term stability. Finally, the proposed method has been successfully applied in the determination of Trp concentration in practical samples.<\/jats:p>","DOI":"10.3390\/biom9050176","type":"journal-article","created":{"date-parts":[[2019,5,13]],"date-time":"2019-05-13T03:57:07Z","timestamp":1557719827000},"page":"176","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":118,"title":["Electrochemical Sensor for Rapid and Sensitive Detection of Tryptophan by a Cu2O Nanoparticles-Coated Reduced Graphene Oxide Nanocomposite"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9849-7953","authenticated-orcid":false,"given":"Quanguo","family":"He","sequence":"first","affiliation":[{"name":"School of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China"},{"name":"Key Laboratory of Functional Metal\u2013Organic Compounds of Hunan Province, Key Laboratory of Functional Organometallic Materials, University of Hunan Province, Department of Chemistry and Material Science, Hengyang Normal University, Hengyang 421008, China"},{"name":"School of Materials Science and Energy Engineering, Foshan University, Foshan 528000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaling","family":"Tian","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China"},{"name":"Key Laboratory of Functional Metal\u2013Organic Compounds of Hunan Province, Key Laboratory of Functional Organometallic Materials, University of Hunan Province, Department of Chemistry and Material Science, Hengyang Normal University, Hengyang 421008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiyong","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China"},{"name":"Key Laboratory of Functional Metal\u2013Organic Compounds of Hunan Province, Key Laboratory of Functional Organometallic Materials, University of Hunan Province, Department of Chemistry and Material Science, Hengyang Normal University, Hengyang 421008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangli","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peihong","family":"Deng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Functional Metal\u2013Organic Compounds of Hunan Province, Key Laboratory of Functional Organometallic Materials, University of Hunan Province, Department of Chemistry and Material Science, Hengyang Normal University, Hengyang 421008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongchu","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Materials Science and Energy Engineering, Foshan University, Foshan 528000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,5,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4022","DOI":"10.1016\/j.electacta.2011.02.009","article-title":"nano-structured Ni(II)\u2013ACDA modified gold nanoparticle self-assembled electrode for electrocatalytic oxidation and determination of tryptophan","volume":"56","author":"Gholivand","year":"2011","journal-title":"Electrochim. Acta"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.colsurfb.2008.06.017","article-title":"Simultaneous determination of tryptophan, uric acid and ascorbic acid at iron(III) doped zeolite modified carbon paste electrode","volume":"66","author":"Babaei","year":"2008","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"He, Q., Liu, J., Liang, J., Liu, X., Li, W., Liu, Z., Ding, Z., and Tuo, D. (2018). Towards Improvements for Penetrating the Blood\u2013Brain Barrier\u2014Recent Progress from a Material and Pharmaceutical Perspective. Cells, 7.","DOI":"10.3390\/cells7040024"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.jpba.2012.03.014","article-title":"Determination of eight amino acids in mice embryonic stem cells by precolumn derivatization HPLC with fluorescence detection","volume":"66","author":"Zhang","year":"2012","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.jchromb.2017.09.020","article-title":"Tryptophan and kynurenine determination in human hair by liquid chromatography","volume":"1065\u20131066","author":"Dario","year":"2017","journal-title":"J. Chromatogr. B"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4535","DOI":"10.1039\/C5AY00190K","article-title":"Determination of enantiomeric composition of tryptophan by using fluorescence spectroscopy combined with backward interval partial least squares","volume":"7","author":"Jiao","year":"2015","journal-title":"Anal. Methods"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1880","DOI":"10.1002\/elps.201400580","article-title":"Microchip electrophoresis-single wall carbon nanotube press-transferred electrodes for fast and reliable electrochemical sensing of melatonin and its precursors","volume":"36","author":"Gomez","year":"2015","journal-title":"Electrophoresis"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1016\/j.talanta.2007.11.049","article-title":"Chemiluminescence of tryptophan and histidine in Ru(bpy)32+-KMnO4 aqueous solution","volume":"75","author":"Lin","year":"2008","journal-title":"Talanta"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.ab.2006.09.004","article-title":"\u03b1-Cyclodextrin-modified infrared chemical sensor for selective determination of tyrosine in biological fluids","volume":"359","author":"Lee","year":"2006","journal-title":"Anal. Biochem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.jelechem.2018.12.056","article-title":"Electrochemical investigation of a graphite-polyurethane composite electrode modified with electrodeposited gold nanoparticles in the voltammetric determination of tryptophan","volume":"835","author":"Mattioli","year":"2019","journal-title":"J. Electroanal. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1016\/j.snb.2018.06.014","article-title":"A highly sensitive electrochemical determination of L-tryptophan in the presence of ascorbic acid and uric acid using in situ addition of tetrabutylammonium bromide on the \u00df-cyclodextrin incorporated multi-walled carbon nanotubes modified electrode","volume":"272","author":"Mukdasai","year":"2018","journal-title":"Sens. Actuators B Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.bios.2011.09.048","article-title":"A novel approach for the selective determination of tryptophan in blood serum in the presence of tyrosine based on the electrochemical reduction of oxidation product of tryptophan formed in situ on graphite electrode","volume":"31","year":"2012","journal-title":"Biosen. Bioelectron."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.snb.2012.09.018","article-title":"Determination of l-tryptophan in the presence of ascorbic acid and dopamine using poly(sulfosalicylic acid) modified glassy carbon electrode","volume":"187","author":"Ba","year":"2013","journal-title":"Sens. Actuators B Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1016\/j.snb.2016.09.119","article-title":"Electrochemical determination of tryptophan using a glassy carbon electrode modified with flower-like structured nanocomposite consisting of reduced graphene oxide and SnO2","volume":"239","author":"Haldorai","year":"2017","journal-title":"Sens. Actuators B Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.colsurfb.2009.11.017","article-title":"Electrochemical investigation of tryptophan at gold nanoparticles modified electrode in the presence of sodium dodecylbenzene sulfonate","volume":"76","author":"Li","year":"2010","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.msec.2013.10.019","article-title":"Acetylene black paste electrode modified with graphene as the voltammetric sensor for selective determination of tryptophan in the presence of high concentrations of tyrosine","volume":"35","author":"Deng","year":"2014","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2182","DOI":"10.1016\/j.talanta.2009.11.027","article-title":"Electrochemical determination of L-Tryptophan, L-Tyrosine and L-Cysteine using electrospun carbon nanofibers modified electrode","volume":"80","author":"Tang","year":"2010","journal-title":"Talanta"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1016\/j.elecom.2004.03.006","article-title":"The electrochemical behavior and amperometric determination of tyrosine and tryptophan at a glassy carbon electrode modified with butyrylcholine","volume":"6","author":"Jin","year":"2004","journal-title":"Electrochem. Commun."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/s00604-003-0133-y","article-title":"Electrocatalysis of tryptophan at multi-walled carbon nanotube modified electrode","volume":"144","author":"Wu","year":"2004","journal-title":"Microchim. Acta"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1016\/j.electacta.2017.12.125","article-title":"Facile synthesis of Pd\u2212Cu@Cu2O\/N-RGO hybrid and its application for electrochemical detection of tryptophan","volume":"260","author":"Li","year":"2018","journal-title":"Electrochim. Acta"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"He, Q., Liu, J., Liu, X., Li, G., Chen, D., Deng, P., and Liang, J. (2018). Fabrication of amine-modified magnetite-electrochemically reduced graphene oxide nanocomposite modified glassy carbon electrode for sensitive dopamine determination. Nanomaterials, 8.","DOI":"10.3390\/nano8040194"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"He, Q., Li, G., Liu, X., Liu, J., Deng, P., and Chen, D. (2018). Morphologically tunable MnO2 nanoparticles fabrication, modelling and their influences on electrochemical sensing performance toward dopamine. Catalysts, 8.","DOI":"10.3390\/catal8080323"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.snb.2014.07.028","article-title":"Dopamine wide range detection sensor based on modified Co3O4 nanowires electrode","volume":"203","author":"Elhag","year":"2014","journal-title":"Sens. Actuators B Chem."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"He, Q., Liu, J., Liu, X., Li, G., Deng, P., Liang, J., and Chen, D. (2018). Sensitive and selective detection of tartrazine based on TiO2-Electrochemically reduced graphene oxide composite-modified electrodes. Sensors, 18.","DOI":"10.3390\/s18061911"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1016\/j.electacta.2017.06.074","article-title":"Enhanced performances of sensors based on screen printed electrodes modified with nanosized NiO particles","volume":"246","author":"Carbone","year":"2017","journal-title":"Electrochim. Acta"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"He, Q., Wu, Y., Tian, Y., Li, G., Liu, J., Deng, P., and Chen, D. (2019). Facile electrochemical sensor for nanomolar rutin detection based on magnetite nanoparticles and reduced graphene oxide decorated electrode. Nanomaterials, 9.","DOI":"10.3390\/nano9010115"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/j.colsurfb.2018.09.005","article-title":"Manganese dioxide Nanorods\/electrochemically reduced graphene oxide nanocomposites modified electrodes for cost-effective and ultrasensitive detection of Amaranth","volume":"172","author":"He","year":"2018","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/j.electacta.2018.11.096","article-title":"A promising sensing platform toward dopamine using MnO2 nanowires\/electro-reduced graphene oxide composites","volume":"296","author":"He","year":"2019","journal-title":"Electrochim. Acta"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/j.solener.2005.10.012","article-title":"Thin film deposition of Cu2O and application for solar cells","volume":"80","author":"Akimoto","year":"2006","journal-title":"Sol. Energy."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1038\/35035045","article-title":"Nano-sized transition-metal oxides as negative-electrode materials for lithium-ion batteries","volume":"407","author":"Poizot","year":"2000","journal-title":"Nature"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2766","DOI":"10.1002\/adfm.200601146","article-title":"One-Pot Synthesis and Hierarchical Assembly of Hollow Cu2O Microspheres with Nanocrystals-Composed Porous Multishell and Their Gas-Sensing Properties","volume":"17","author":"Zhang","year":"2007","journal-title":"Adv. Funct. Mater."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1166\/sam.2010.1095","article-title":"Synthesis of TiO2 and CuO nanotubes and nanowires","volume":"2","author":"Singh","year":"2010","journal-title":"Sci. Adv. Mater."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"7686","DOI":"10.1016\/j.electacta.2011.06.053","article-title":"Fabrication of nanometer-to-micron sized Cu2O single crystals by electrodeposition","volume":"56","author":"Ng","year":"2011","journal-title":"Electrochim. Acta"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1039\/b311625e","article-title":"Controlling the size of Cu2O nanocubes from 200 to 25 nm","volume":"14","author":"Gou","year":"2004","journal-title":"J. Mater. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1021\/cm052256f","article-title":"Nearly monodisperse Cu2O and CuO nanospheres: Preparation and applications for sensitive gas sensors","volume":"18","author":"Zhang","year":"2006","journal-title":"Chem. Mater."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"He, Q., Tian, Y., Wu, Y., Liu, J., Li, G., Deng, P., and Chen, D. (2019). Facile and Ultrasensitive Determination of 4-Nitrophenol Based on Acetylene Black Paste and Graphene Hybrid Electrode. Nanomaterials, 9.","DOI":"10.3390\/nano9030429"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.foodchem.2015.02.035","article-title":"Electrochemical behavior and voltammetric determination of vanillin based on an acetylene black paste electrode modified with grapheme\u2013polyvinylpyrrolidone composite film","volume":"180","author":"Deng","year":"2015","journal-title":"Food Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.bios.2016.11.016","article-title":"Ag\/N-doped reduced graphene oxide incorporated with molecularly imprinted polymer: An advanced electrochemical sensing platform for salbutamol determination","volume":"90","author":"Li","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"He, Q., Liu, J., Liu, X., Li, G., Deng, P., and Liang, J. (2018). Preparation of Cu2O-reduced graphene nanocomposite modified electrodes towards ultrasensitive dopamine detection. Sensors, 18.","DOI":"10.3390\/s18010199"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/s00604-010-0535-6","article-title":"One-pot solvothermal synthesis of a Cu2O\/Graphene nanocomposite and its application in an electrochemical sensor for dopamine","volume":"173","author":"Zhang","year":"2011","journal-title":"Microchim. Acta"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.electacta.2012.10.070","article-title":"Electrochemical behavior of cuprous oxide\u2013reduced graphene oxide nanocomposites and their application in nonenzymatic hydrogen peroxide sensing","volume":"88","author":"Xu","year":"2013","journal-title":"Electrochim. Acta"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/j.snb.2014.09.016","article-title":"Carbon quantum dots\/octahedral Cu2O nanocomposites for non-enzymatic glucose and hydrogen peroxide amperometric sensor","volume":"206","author":"Li","year":"2015","journal-title":"Sens. Actuators B"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.snb.2015.03.026","article-title":"A non-enzymatic glucose sensing based on hollow cuprous oxide nanospheres in a Nafion matrix","volume":"214","author":"Cao","year":"2015","journal-title":"Sens. Actuators B"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.electacta.2013.04.073","article-title":"Cu2O\/NiOx\/graphene oxide modified glassy carbon electrode for the enhanced electrochemical oxidation of reduced glutathione and nonenzyme glucose sensor","volume":"104","author":"Yuan","year":"2013","journal-title":"Electrochim. Acta"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1039\/B917975E","article-title":"Facile and controllable electrochemical reduction of graphene oxide and its applications","volume":"20","author":"Shao","year":"2010","journal-title":"J. Mater. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.elecom.2010.11.033","article-title":"Direct electrodeposition of reduced graphene oxide on glassy carbon electrode and its electrochemical application","volume":"13","author":"Chen","year":"2011","journal-title":"Electrochem. Commun."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2653","DOI":"10.1021\/nn900227d","article-title":"A green approach to the synthesis of graphene nanosheets","volume":"3","author":"Guo","year":"2009","journal-title":"ACS Nano"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.snb.2012.06.088","article-title":"Highly sensitive amperometric sensor for carbamazepine determination based on electrochemically reduced graphene oxide\u2013single-walled carbon nanotube composite film","volume":"173","author":"Unnikrishnan","year":"2012","journal-title":"Sens. Actuators B"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1016\/j.jcis.2009.02.036","article-title":"Photochemical synthesis of submicron- and nano-scale Cu2O particles","volume":"333","author":"Long","year":"2009","journal-title":"J. Colloid Interface Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/S0022-0728(74)80448-1","article-title":"Adsorption, autoinhibition and autocatalysis in polarography and in linear potential sweep voltammetry","volume":"52","author":"Laviron","year":"1974","journal-title":"J. Electroanal. Chem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/S0003-2670(02)00496-8","article-title":"Voltammetric behavior and determination of estrogens at Nafion-modified glassy carbon electrode in the presence of cetyltrimethylammonium bromide","volume":"464","author":"Hu","year":"2002","journal-title":"Anal. Chim. Acta"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"5211","DOI":"10.1039\/c1an15351j","article-title":"Sensitive voltammetric determination of tryptophan using an acetylene black paste electrode modified with a Schiff\u2019s base derivative of chitosan","volume":"136","author":"Deng","year":"2011","journal-title":"Analyst"}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/9\/5\/176\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:50:14Z","timestamp":1760187014000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/9\/5\/176"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,8]]},"references-count":52,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2019,5]]}},"alternative-id":["biom9050176"],"URL":"https:\/\/doi.org\/10.3390\/biom9050176","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,8]]}}}