{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T18:19:43Z","timestamp":1771006783445,"version":"3.50.1"},"reference-count":30,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2019,10,17]],"date-time":"2019-10-17T00:00:00Z","timestamp":1571270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/DTP\/04567\/2016"],"award-info":[{"award-number":["UID\/DTP\/04567\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["CBIOS\/PRUID\/BI1\/2017"],"award-info":[{"award-number":["CBIOS\/PRUID\/BI1\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"undefined  &lt;span style=&quot;color:gray;font-size:10px;&quot;&gt;undefined&lt;\/span&gt;","award":["REDE\/1518\/REM\/2005"],"award-info":[{"award-number":["REDE\/1518\/REM\/2005"]}]},{"name":"undefined  &lt;span style=&quot;color:gray;font-size:10px;&quot;&gt;undefined&lt;\/span&gt;","award":["PADDIC 2016 and 2017"],"award-info":[{"award-number":["PADDIC 2016 and 2017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>The Plectranthus genus is commonly used in traditional medicine due to its potential to treat several illnesses, including bacterial infections and cancer. As such, aiming to screen the antibacterial and cytotoxic activities of extracts, sixteen selected Plectranthus species with medicinal potential were studied. In total, 31 extracts obtained from 16 Plectranthus spp. were tested for their antibacterial and anticancer properties. Well diffusion method was used for preliminary antibacterial screening. The minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values of the five most active acetonic extracts (P. aliciae, P. japonicus, P. madagascariensis var. \u201cLynne\u201d, P. stylesii, and P. strigosus) were determined. After preliminary toxicity evaluation on Artemia salina L., their cytotoxic properties were assessed on three human cancer cell lines (HCT116, MCF-7, and H460). These were also selected for mechanism of resistance studies (on NCI-H460\/R and DLD1-TxR cells). An identified compound\u2014parvifloron D\u2014was tested in a pair of sensitive and MDR-Multidrug resistance cancer cells (NCI-H460 and NCI-H460\/R) and in normal bronchial fibroblasts MRC-5. The chemical composition of the most active extract was studied through high performance liquid chromatography with a diode array detector (HPLC-DAD\/UV) and liquid chromatography\u2013mass spectrometry (LC\u2013MS). Overall, P. strigosus acetonic extract showed the strongest antimicrobial and cytotoxic potential that could be explained by the presence of parvifloron D, a highly cytotoxic diterpene. This study provides valuable information on the use of the Plectranthus genus as a source of bioactive compounds, namely P. strigosus with the potential active ingredient the parvifloron D.<\/jats:p>","DOI":"10.3390\/biom9100616","type":"journal-article","created":{"date-parts":[[2019,10,17]],"date-time":"2019-10-17T11:07:59Z","timestamp":1571310479000},"page":"616","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Parvifloron D from Plectranthus strigosus: Cytotoxicity Screening of Plectranthus spp. Extracts"],"prefix":"10.3390","volume":"9","author":[{"given":"Catarina","family":"Garcia","sequence":"first","affiliation":[{"name":"Research Center for Biosciences &amp; Health Technologies (CBIOS), Universidade Lus\u00f3fona de Humanidades e Tecnologias, 1749-024 Lisboa, Portugal"},{"name":"Department of Biomedical Sciences, Faculty of Pharmacy, University of Alcal\u00e1, Campus Universitario, 28871 Alcal\u00e1 de Henares, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6596-8985","authenticated-orcid":false,"given":"Epole","family":"Ntungwe","sequence":"additional","affiliation":[{"name":"Research Center for Biosciences &amp; Health Technologies (CBIOS), Universidade Lus\u00f3fona de Humanidades e Tecnologias, 1749-024 Lisboa, Portugal"},{"name":"Department of Biomedical Sciences, Faculty of Pharmacy, University of Alcal\u00e1, Campus Universitario, 28871 Alcal\u00e1 de Henares, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Rebelo","sequence":"additional","affiliation":[{"name":"Research Center for Biosciences &amp; Health Technologies (CBIOS), Universidade Lus\u00f3fona de Humanidades e Tecnologias, 1749-024 Lisboa, Portugal"},{"name":"Department of Biomedical Sciences, Faculty of Pharmacy, University of Alcal\u00e1, Campus Universitario, 28871 Alcal\u00e1 de Henares, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6240-2329","authenticated-orcid":false,"given":"Cl\u00e1udia","family":"Bessa","sequence":"additional","affiliation":[{"name":"LAQV\/REQUIMTE, Laborat\u00f3rio de Microbiologia, Departamento de Ci\u00eancias Biol\u00f3gicas, Faculdade de Farm\u00e1cia, Universidade do Porto, Rua de Jorge Viterbo Ferreira n. 228, 4050-313 Porto, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tijana","family":"Stankovic","sequence":"additional","affiliation":[{"name":"Institute for Biological Research, \u201cSini\u0161a Stankovi\u0107\u201d, University of Belgrade, Despota Stefana 142, 11060 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3371-2381","authenticated-orcid":false,"given":"Jelena","family":"Dinic","sequence":"additional","affiliation":[{"name":"Institute for Biological Research, \u201cSini\u0161a Stankovi\u0107\u201d, University of Belgrade, Despota Stefana 142, 11060 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4216-3997","authenticated-orcid":false,"given":"Ana","family":"D\u00edaz-Lanza","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Faculty of Pharmacy, University of Alcal\u00e1, Campus Universitario, 28871 Alcal\u00e1 de Henares, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1046-4031","authenticated-orcid":false,"given":"Catarina","family":"P. 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