{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T17:20:16Z","timestamp":1789665616060,"version":"4.0.1"},"reference-count":54,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2019,10,22]],"date-time":"2019-10-22T00:00:00Z","timestamp":1571702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fund for Scientific Research-Flanders","award":["Grants G012318N, G020018N and G0F7219N"],"award-info":[{"award-number":["Grants G012318N, G020018N and G0F7219N"]}]},{"name":"University Hospital of the Vrije Universiteit Brussel-Belgium","award":["Willy Gepts Funds Grant"],"award-info":[{"award-number":["Willy Gepts Funds Grant"]}]},{"name":"University of S\u00e3o Paulo-Brazil and the Foundation for Research Support of the State of S\u00e3o Paulo","award":["FAPESP SPEC grant 2013\/50420-6"],"award-info":[{"award-number":["FAPESP SPEC grant 2013\/50420-6"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Adherens junctions, consisting of cadherins and catenins, are a group of cell-to-cell junctions that mediate mechanistic linkage between neighboring cells. By doing so, adherens junctions ensure direct intercellular contact and play an indispensable role in maintaining tissue architecture. Considering these critical functions, it is not surprising that adherens junctions are frequently involved in disease. In the present study, the effects of bile duct ligation\u2014a surgical procedure to experimentally induce cholestatic and fibrotic liver pathology\u2014on hepatic adherens junctions were investigated in mice. In essence, it was found that liver mRNA and protein levels of E-cadherin, \u03b2-catenin and \u03b3-catenin drastically increase following bile duct ligation. These results could suggest a cytoprotective role for hepatic adherens junctions following bile duct ligation.<\/jats:p>","DOI":"10.3390\/biom9100636","type":"journal-article","created":{"date-parts":[[2019,10,23]],"date-time":"2019-10-23T11:46:59Z","timestamp":1571831219000},"page":"636","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Increased Expression of Adherens Junction Components in Mouse Liver following Bile Duct Ligation"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4340-0210","authenticated-orcid":false,"given":"Raf","family":"Van Campenhout","sequence":"first","affiliation":[{"name":"Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3195-6817","authenticated-orcid":false,"given":"Sara","family":"Crespo Yanguas","sequence":"additional","affiliation":[{"name":"Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axelle","family":"Cooreman","sequence":"additional","affiliation":[{"name":"Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Gijbels","sequence":"additional","affiliation":[{"name":"Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2900-0437","authenticated-orcid":false,"given":"Kaat","family":"Leroy","sequence":"additional","affiliation":[{"name":"Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e2nia","family":"Vilas-Boas","sequence":"additional","affiliation":[{"name":"Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9220-4833","authenticated-orcid":false,"given":"Nick","family":"Devoogdt","sequence":"additional","affiliation":[{"name":"In Vivo Cellular and Molecular Imaging Laboratory, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3678-3575","authenticated-orcid":false,"given":"Serge","family":"Muyldermans","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Cogliati","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, 05508-270 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Vinken","sequence":"additional","affiliation":[{"name":"Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,10,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"a002899","DOI":"10.1101\/cshperspect.a002899","article-title":"Adherens junction: Molecular architecture and regulation","volume":"1","author":"Meng","year":"2009","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Yang, X., Chung, J.Y., Rai, U., and Esumi, N. (2018). Cadherins in the retinal pigment epithelium (RPE) revisited: P-cadherin is the highly dominant cadherin expressed in human and mouse RPE in vivo. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0191279"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1080\/10408440600599273","article-title":"Involvement of cell junctions in hepatocyte culture functionality","volume":"36","author":"Vinken","year":"2006","journal-title":"Crit. Rev. Toxicol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1093\/toxsci\/61.2.389","article-title":"Selective disruption of cadherin\/catenin complexes by oxidative stress in precision-cut mouse liver slices","volume":"61","author":"Schmelz","year":"2001","journal-title":"Toxicol. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1002\/bies.1045","article-title":"Wnt signalling: A theme with nuclear variations","volume":"23","author":"Sharpe","year":"2001","journal-title":"Bioessays"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1016\/S0002-9440(10)62550-3","article-title":"Role of \u03b2-catenin\/T-cell factor-regulated genes in ovarian endometrioid adenocarcinomas","volume":"160","author":"Zhai","year":"2002","journal-title":"Am. J. Pathol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S1359-6101(00)00011-3","article-title":"Wnt\/\u03b2-catenin signaling","volume":"11","author":"Akiyama","year":"2000","journal-title":"Cytokine Growth Factor Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1016\/j.devcel.2009.11.006","article-title":"Cell cycle control of Wnt receptor activation","volume":"17","author":"Davidson","year":"2009","journal-title":"Dev. Cell"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0304-419X(03)00005-2","article-title":"Caught up in a Wnt storm: Wnt signaling in cancer","volume":"1653","author":"Giles","year":"2003","journal-title":"Biochim. Biophys. Acta Rev. Cancer"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.devcel.2009.06.016","article-title":"Wnt\/\u03b2-catenin signaling: Components, mechanisms, and diseases","volume":"17","author":"MacDonald","year":"2009","journal-title":"Dev. Cell"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.2353\/ajpath.2009.080976","article-title":"Beta-catenin activation promotes liver regeneration after acetaminophen-induced injury","volume":"175","author":"Apte","year":"2009","journal-title":"Am. J. Pathol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2599","DOI":"10.1007\/s00018-015-1861-y","article-title":"Pathological implications of cadherin zonation in mouse liver","volume":"72","author":"Hempel","year":"2015","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.3892\/mmr.2014.2099","article-title":"\u03b2-catenin is overexpressed in hepatic fibrosis and blockage of Wnt\/\u03b2-catenin signaling inhibits hepatic stellate cell activation","volume":"9","author":"Ge","year":"2014","journal-title":"Mol. Med. Rep."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1053\/hp.2000.6683","article-title":"Immunoreactive E-cadherin, alpha-catenin, beta-catenin, and gamma-catenin proteins in hepatocellular carcinoma: Relationships with tumor grade, clinicopathologic parameters, and patients\u2019 survival","volume":"31","author":"Endo","year":"2000","journal-title":"Hum. Pathol."},{"key":"ref_15","first-page":"e52438","article-title":"Bile duct ligation in mice: induction of inflammatory liver injury and fibrosis by obstructive cholestasis","volume":"96","author":"Tag","year":"2015","journal-title":"J. Vis. Exp."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2607","DOI":"10.1007\/s00204-018-2255-3","article-title":"Genetic ablation of pannexin1 counteracts liver fibrosis in a chemical, but not in a surgical mouse model","volume":"92","author":"Yanguas","year":"2018","journal-title":"Arch. Toxicol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1002\/jemt.20926","article-title":"Morphological and molecular pathology of CCl4-induced hepatic fibrosis in connexin43-deficient mice","volume":"74","author":"Cogliati","year":"2011","journal-title":"Microsc. Res. Tech."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1002\/mrd.22489","article-title":"The ImageJ ecosystem: An open platform for biomedical image analysis","volume":"82","author":"Schindelin","year":"2015","journal-title":"Mol. Reprod. Dev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2245","DOI":"10.1007\/s00204-016-1885-6","article-title":"Inhibition of pannexin1 channels alleviates acetaminophen-induced hepatotoxicity","volume":"91","author":"Maes","year":"2017","journal-title":"Arch. Toxicol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Eaton, S.L., Roche, S.L., Hurtado, M.L., Oldknow, K.J., Farquharson, C., Gillingwater, T.H., and Wishart, T.M. (2013). Total protein analysis as a reliable loading control for quantitative fluorescent Western blotting. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0072457"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ab.2014.08.027","article-title":"Stain-free detection as loading control alternative to Ponceau and housekeeping protein immunodetection in Western blotting","volume":"467","author":"Anzola","year":"2014","journal-title":"Anal. Biochem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1007\/s12033-013-9672-6","article-title":"A defined methodology for reliable quantification of western blot data","volume":"55","author":"Taylor","year":"2013","journal-title":"Mol. Biotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2014\/361590","article-title":"The design of a quantitative western blot experiment","volume":"2014","author":"Taylor","year":"2014","journal-title":"Biomed Res. Int."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1007\/s00204-015-1543-4","article-title":"Experimental models of liver fibrosis","volume":"90","author":"Yanguas","year":"2016","journal-title":"Arch. Toxicol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1002\/bjs.6050","article-title":"Characterization of time-related changes after experimental bile duct ligation","volume":"95","author":"Georgiev","year":"2008","journal-title":"Br. J. Surg."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1007\/s00418-007-0374-7","article-title":"Bile duct ligation in the rat causes upregulation of ZO-2 and decreased colocalization of claudins with ZO\u22121 and occludin","volume":"129","author":"Maly","year":"2008","journal-title":"Histochem. Cell Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3274","DOI":"10.1016\/j.ajpath.2015.08.019","article-title":"Mice with hepatic loss of the desmosomal protein \u03b3-catenin are prone to cholestatic injury and chemical carcinogenesis","volume":"185","author":"Zhou","year":"2015","journal-title":"Am. J. Pathol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"R1249","DOI":"10.1152\/ajpregu.1999.276.5.R1249","article-title":"Cx32 mRNA in rat liver: Effects of inflammation on poly(A) tail distribution and mRNA degradation","volume":"276","author":"Theodorakis","year":"2017","journal-title":"Am. J. Physiol. Integr. Comp. Physiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1053\/j.gastro.2015.02.056","article-title":"\u03b2-catenin signaling and roles in liver homeostasis, injury, and tumorigenesis","volume":"148","author":"Monga","year":"2015","journal-title":"Gastroenterology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1093\/emboj\/21.5.1021","article-title":"K-Ras mediates cytokine-induced formation of E-cadherin-based adherens junctions during liver development","volume":"21","author":"Matsui","year":"2008","journal-title":"EMBO J."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2007","DOI":"10.1002\/hep.23196","article-title":"Epithelial-to-mesenchymal transitions in the liver","volume":"50","author":"Choi","year":"2009","journal-title":"Hepatology"},{"key":"ref_33","first-page":"G881","article-title":"Evidence for and against epithelial-to-mesenchymal transition in the liver","volume":"305","author":"Xie","year":"2013","journal-title":"Am. J. Physiol. Liver Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1380","DOI":"10.3748\/wjg.v19.i9.1380","article-title":"Hepatic regeneration and the epithelial to mesenchymal transition","volume":"19","author":"Xue","year":"2013","journal-title":"World J. Gastroenterol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1172\/JCI39104","article-title":"The basics of epithelial-mesenchymal transition","volume":"119","author":"Kalluri","year":"2011","journal-title":"J. Clin. Investig."},{"key":"ref_36","first-page":"737","article-title":"The epithelial-to-mesenchymal transition in liver fibrosis: Here today, gone tomorrow?","volume":"51","author":"Wells","year":"2010","journal-title":"Hepatology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1500","DOI":"10.2353\/ajpath.2006.050747","article-title":"Hepatocyte growth factor attenuates liver fibrosis induced by bile duct ligation","volume":"168","author":"Xia","year":"2006","journal-title":"Am. J. Pathol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"23337","DOI":"10.1074\/jbc.M700194200","article-title":"Fibroblasts derive from hepatocytes in liver fibrosis via epithelial to mesenchymal transition","volume":"282","author":"Zeisberg","year":"2007","journal-title":"J. Biol. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1038\/nrm1835","article-title":"Complex networks orchestrate epithelial-mesenchymal transitions","volume":"7","author":"Thiery","year":"2006","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1172\/JCI36183","article-title":"Review series personal perspective biomarkers for epithelial-mesenchymal transitions","volume":"119","author":"Zeisberg","year":"2009","journal-title":"J. Clin. Investig."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"269","DOI":"10.3892\/br.2016.578","article-title":"Epithelial-mesenchymal transition in liver fibrosis (review)","volume":"4","author":"Zhao","year":"2016","journal-title":"Biomed. Rep."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1038\/labinvest.3700704","article-title":"Epithelial-mesenchymal transition contributes to portal tract fibrogenesis during human chronic liver disease","volume":"88","author":"Rygiel","year":"2008","journal-title":"Lab. Investig."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"32270","DOI":"10.18632\/oncotarget.15650","article-title":"Beyond cell-cell adhesion: Plakoglobin and the regulation of tumorigenesis and metastasis","volume":"8","author":"Aktary","year":"2017","journal-title":"Oncotarget"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1038\/sj.onc.1207254","article-title":"Plakoglobin (\u03b3-catenin) has TCF\/LEF family-dependent transcriptional activity in \u03b2-catenin-deficient cell line","volume":"23","author":"Maeda","year":"2004","journal-title":"Oncogene"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1016\/j.jhep.2011.03.014","article-title":"Hepatocyte \u03b3-catenin compensates for conditionally deleted \u03b2-catenin at adherens junctions","volume":"55","author":"Wickline","year":"2011","journal-title":"J. Hepatol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1101\/gad.14.11.1319","article-title":"\u03b3-Catenin is regulated by the APC tumor suppressor and its oncogenic activity is distinct from that of \u03b2-catenin","volume":"14","author":"Kolligs","year":"2000","journal-title":"Genes Dev."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/S0955-0674(97)80122-6","article-title":"Cadherins, catenins and APC protein: Interplay between cytoskeletal complexes and signaling pathways","volume":"9","author":"Barth","year":"1997","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1652","DOI":"10.1002\/hep.28774","article-title":"Wnt signaling regulates hepatobiliary repair following cholestatic liver injury in mice","volume":"64","author":"Okabe","year":"2016","journal-title":"Hepatology"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1002\/hep.21651","article-title":"WNT\/\u03b2-catenin signaling in liver health and disease","volume":"45","author":"Thompson","year":"2007","journal-title":"Hepatology"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1016\/j.jhep.2017.12.011","article-title":"Plectin controls biliary tree architecture and stability in cholestasis","volume":"68","author":"Jirouskova","year":"2018","journal-title":"J. Hepatol."},{"key":"ref_51","first-page":"69","article-title":"Review blood\u2014Bile barrier: Morphology, regulation, and pathophysiology","volume":"19","author":"Monga","year":"2019","journal-title":"Gene Expr. J. Liver Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1016\/S1673-8527(07)60101-5","article-title":"Expression patterns of the cell junction-associated genes during rat liver regeneration","volume":"34","author":"Li","year":"2007","journal-title":"J. Genet. Genom."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/0014-4827(92)90102-E","article-title":"Regulation of adherens junction protein expression in growth-activated 3T3 cells and in regenerating liver","volume":"202","author":"Pankov","year":"1992","journal-title":"Exp. Cell Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"6835","DOI":"10.1038\/srep06835","article-title":"Loss of \u03b1-catenin elicits a cholestatic response and impairs liver regeneration","volume":"4","author":"Herr","year":"2014","journal-title":"Sci. Rep."}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/9\/10\/636\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:28:28Z","timestamp":1760189308000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/9\/10\/636"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,22]]},"references-count":54,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2019,10]]}},"alternative-id":["biom9100636"],"URL":"https:\/\/doi.org\/10.3390\/biom9100636","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,22]]}}}