{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T10:04:54Z","timestamp":1769767494225,"version":"3.49.0"},"reference-count":46,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2019,10,29]],"date-time":"2019-10-29T00:00:00Z","timestamp":1572307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005672","name":"Funda\u00e7\u00e3o de Apoio ao Desenvolvimento do Ensino, Ci\u00eancia e Tecnologia do Estado de Mato Grosso do Sul","doi-asserted-by":"publisher","award":["59\/300.046\/2015"],"award-info":[{"award-number":["59\/300.046\/2015"]}],"id":[{"id":"10.13039\/501100005672","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["449464\/2014-8 and 407685\/2018-9"],"award-info":[{"award-number":["449464\/2014-8 and 407685\/2018-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Therapeutic approaches for the treatment of dyslipidemia and atherosclerosis have radically changed in recent decades. Part of this advance undeniably stems from basic biomedical research that has provided a better understanding and identification of new therapeutic targets. The aim of this work was to develop a model to induce atherogenesis and hepato-renal impairment in female Wistar rats. The following groups received the respective treatments for 60 days: control animals, non-ovariectomized rats that received an atherogenic diet (NEAD), ovariectomized rats that received an atherogenic diet (NOAD), non-ovariectomized rats that received an atherogenic diet and oral N\u03c9-nitro-l-arginine methyl ester hydrochloride (l-NAME; LEAD), and ovariectomized rats that received an atherogenic diet and oral l-NAME (LOAD). Animals in the NEAD, NOAD, LEAD, and LOAD groups also received methimazole and cholecalciferol daily. Urinary, biochemical, hemodynamic, and electrocardiographic parameters and renal function were assessed. Samples of the liver, heart, kidney, and arteries were collected to investigate redox status and perform histopathological analyses. All of the groups developed dyslipidemia and hepatic steatosis. Only the NEAD group developed arterial lesions that were compatible with fatty streaks. Renal function was significantly impaired in the LEAD and NOAD groups. These results indicate a viable alternative to induce atherogenesis and hepato-renal impairment in female rats.<\/jats:p>","DOI":"10.3390\/biom9110664","type":"journal-article","created":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T05:18:26Z","timestamp":1572499106000},"page":"664","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0733-1975","authenticated-orcid":false,"given":"Lucas Pires","family":"Guarnier","sequence":"first","affiliation":[{"name":"Laboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS 79825-070, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Vitor Moreira","family":"Rom\u00e3o","sequence":"additional","affiliation":[{"name":"Laboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS 79825-070, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8941-6592","authenticated-orcid":false,"given":"Rhanany Alan Calloi","family":"Palozi","sequence":"additional","affiliation":[{"name":"Laboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS 79825-070, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aniely Oliveira","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS 79825-070, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beth\u00e2nia Rosa","family":"Loren\u00e7one","sequence":"additional","affiliation":[{"name":"Laboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS 79825-070, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aline Aparecida Macedo","family":"Marques","sequence":"additional","affiliation":[{"name":"Laboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS 79825-070, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ariany Carvalho","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS 79825-070, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roosevelt Isaias Carvalho","family":"Souza","sequence":"additional","affiliation":[{"name":"Laboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS 79825-070, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karine Delgado","family":"Souza","sequence":"additional","affiliation":[{"name":"Laboratory of Preclinical Research of Natural Products, Paranaense University (UNIPAR), Umuarama, PR 87502-210, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emerson Luiz Botelho","family":"Louren\u00e7o","sequence":"additional","affiliation":[{"name":"Laboratory of Preclinical Research of Natural Products, Paranaense University (UNIPAR), Umuarama, PR 87502-210, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3433-5098","authenticated-orcid":false,"given":"Arquimedes","family":"Gasparotto Junior","sequence":"additional","affiliation":[{"name":"Laboratory of Electrophysiology and Cardiovascular Pharmacology, Faculty of Health Sciences, Federal University of Grande Dourados (UFGD), Dourados, MS 79825-070, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,10,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.jep.2011.03.045","article-title":"Effects of total glucosides from paeony (Paeonia lactiflora Pall) roots on experimental atherosclerosis in rats","volume":"135","author":"Li","year":"2011","journal-title":"J. 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