{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:55:54Z","timestamp":1788350154846,"version":"build-2803163510"},"reference-count":51,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T00:00:00Z","timestamp":1574726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2017M1A5A1013569"],"award-info":[{"award-number":["NRF-2017M1A5A1013569"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2018R1D1A1B07044447"],"award-info":[{"award-number":["NRF-2018R1D1A1B07044447"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Molecular information about family VIII esterases, which have similarities with class C \u03b2-lactamases and penicillin-binding proteins, remains largely unknown. In this study, a novel family VIII esterase with \u03b2-lactamase activity (PsEstA) from Paenibacillus sp. was characterized using several biochemical and biophysical methods. PsEstA was effective on a broad range of substrates including tertiary butyl acetate, glyceryl tributyrate, glucose pentaacetate, olive oil, and p-nitrophenyl esters. Additionally, PsEstA hydrolyzed nitrocefin, cefotaxime, and 7-aminocephalosporanic acid. Interestingly, two forms of immobilized PsEstA (CLEAs-PsEstA and mCLEAs-PsEstA) showed high recycling property and enhanced stability, but hybrid nanoflowers (hNFs) of PsEstA require improvement. This study provides a molecular understanding of substrate specificities, catalytic regulation, and immobilization of PsEstA, which can be efficiently used in biotechnological applications.<\/jats:p>","DOI":"10.3390\/biom9120786","type":"journal-article","created":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T10:57:27Z","timestamp":1574765847000},"page":"786","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Molecular Characterization of a Novel Family VIII Esterase with \u03b2-Lactamase Activity (PsEstA) from Paenibacillus sp."],"prefix":"10.3390","volume":"9","author":[{"given":"Sena","family":"Kwon","sequence":"first","affiliation":[{"name":"Department of Chemistry, College of Natural Science, Sookmyung Women\u2019s University, Seoul 04310, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanki","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Natural Science, Sookmyung Women\u2019s University, Seoul 04310, Korea"},{"name":"Department of Molecular Cell Biology, SungKyunKwan University School of Medicine, Suwon 2066, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young-Ok","family":"Kim","sequence":"additional","affiliation":[{"name":"Biotechnology Research Division, National Fisheries Research and Development Institute, Gijang, Busan 46083, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2515-8894","authenticated-orcid":false,"given":"Kyeong Kyu","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology, SungKyunKwan University School of Medicine, Suwon 2066, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. Doohun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Natural Science, Sookmyung Women\u2019s University, Seoul 04310, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,11,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1021\/cr030102i","article-title":"Bacterial resistance to beta-lactam antibiotics: Compelling opportunism, compelling opportunity","volume":"105","author":"Fisher","year":"2005","journal-title":"Chem. Rev."},{"key":"ref_2","first-page":"130","article-title":"Structure, function of serine and metallo-\u03b2-lactamases and their inhibitors","volume":"19","author":"Salahuddin","year":"2018","journal-title":"Curr. Protein Pept. 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