{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T21:18:00Z","timestamp":1792876680472,"version":"4.5.0"},"reference-count":45,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T00:00:00Z","timestamp":1575849600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Nature Science Foundation of China","award":["31801139"],"award-info":[{"award-number":["31801139"]}]},{"name":"Chongqing Science and Technology Bureau","award":["cstc2018jcyjAX0120"],"award-info":[{"award-number":["cstc2018jcyjAX0120"]}]},{"name":"Chongqing Science and Technology Bureau","award":["cstc2016jcyjA0572"],"award-info":[{"award-number":["cstc2016jcyjA0572"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Neuropathy target esterase (NTE) is an endoplasmic reticulum (ER)-localized phospholipase that deacylates phosphatidylcholine (PC) and lysophosphatidylcholine (LPC). Loss-of-function mutations in the human NTE gene have been associated with a spectrum of neurodegenerative disorders such as hereditary spastic paraplegia, ataxia and chorioretinal dystrophy. Despite this, little is known about structure\u2013function relationships between NTE protein domains, enzymatic activity and the interaction with cellular organelles. In the current study we show that the C-terminal region of NTE forms a catalytically active domain that exhibits high affinity for lipid droplets (LDs), cellular storage organelles for triacylglycerol (TAG), which have been recently implicated in the progression of neurodegenerative diseases. Ectopic expression of the C domain in cultured cells decreases cellular PC, elevates TAG and induces LD clustering. LD interactions of NTE are inhibited by default by a non-enzymatic regulatory (R) region with three putative nucleotide monophosphate binding sites. Together with a N-terminal TMD the R region promotes proper distribution of the catalytic C-terminal region to the ER network. Taken together, our data indicate that NTE may exhibit dynamic interactions with the ER and LDs depending on the interplay of its functional regions. Mutations that disrupt this interplay may contribute to NTE-associated disorders by affecting NTE positioning.<\/jats:p>","DOI":"10.3390\/biom9120848","type":"journal-article","created":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T11:22:51Z","timestamp":1575890571000},"page":"848","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Characterization of the Interaction of Neuropathy Target Esterase with the Endoplasmic Reticulum and Lipid Droplets"],"prefix":"10.3390","volume":"9","author":[{"given":"Pingan","family":"Chang","sequence":"first","affiliation":[{"name":"Chongqing Key Laboratory of Big Data for Bio-Intelligence, School of Bio-information, Chongqing University of Posts and Telecommunications, Chongqing 400065, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"He","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Big Data for Bio-Intelligence, School of Bio-information, Chongqing University of Posts and Telecommunications, Chongqing 400065, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Big Data for Bio-Intelligence, School of Bio-information, Chongqing University of Posts and Telecommunications, Chongqing 400065, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Heier","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijun","family":"Wu","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feifei","family":"Huang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Big Data for Bio-Intelligence, School of Bio-information, Chongqing University of Posts and Telecommunications, Chongqing 400065, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.cbi.2012.10.024","article-title":"Neuropathy target esterase (NTE): Overview and future","volume":"203","author":"Richardson","year":"2013","journal-title":"Chem. 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