{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:33:53Z","timestamp":1777379633787,"version":"3.51.4"},"reference-count":40,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T00:00:00Z","timestamp":1777161600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deanship of Scientific Research (DSR) at King Abdulaziz University","award":["G: 343-155-1443"],"award-info":[{"award-number":["G: 343-155-1443"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomimetics"],"abstract":"<jats:p>Decellularized extracellular matrix (dECM) scaffolds derived from xenogeneic tissues represent promising biomaterials for tissue engineering. In this study, dECM scaffolds were developed and characterized from four ovine tissues\u2014skin, tunica vaginalis, fascia lata, and pericardium\u2014using a detergent-based decellularization protocol to evaluate decellularization efficiency and extracellular matrix (ECM) preservation. Decellularization was performed using a sequential detergent-based protocol with sodium dodecyl sulfate and Triton X-100. Decellularization efficacy and matrix preservation were evaluated through gross examination, histological analysis, scanning electron microscopy (SEM), and residual DNA quantification. Gross inspection revealed increased translucency and reduced pigmentation in decellularized tissues compared with native counterparts, indicating effective cellular removal while maintaining overall tissue architecture. Histological assessment confirmed the complete absence of nuclear and cytoplasmic material, alongside preservation of collagen-rich extracellular matrix organization. SEM analysis demonstrated well-maintained ultrastructural features, including aligned collagen fibers and porous ECM architecture, with complete removal of epithelial and stromal cellular elements. Quantitative analysis revealed approximately 94% reduction in residual DNA content across all decellularized tissues compared with native controls. This study demonstrated that the employed detergent-based protocol reliably produces structurally preserved, acellular scaffolds from multiple ovine tissues. The resulting biomaterials exhibit structural characteristics that support their potential use in tissue engineering applications, pending further functional validation.<\/jats:p>","DOI":"10.3390\/biomimetics11050301","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T08:12:31Z","timestamp":1777363951000},"page":"301","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Detergent-Based Decellularization Preserves Extracellular Matrix Ultrastructure in Ovine Soft Tissues"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2378-7961","authenticated-orcid":false,"given":"Ibrahim E.","family":"Helal","sequence":"first","affiliation":[{"name":"Department of Agriculture, Faculty of Environment Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4654-5909","authenticated-orcid":false,"given":"Mahmoud F.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Suez Canal University, 4.5 Ring Road, Ismailia 41522, Egypt"},{"name":"Department of Surgery and Theriogenology, Faculty of Veterinary Medicine, King Salman International University, Ras Sudr 46612, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9235-2449","authenticated-orcid":false,"given":"Ahmed M.","family":"Abdellatif","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed A.","family":"Hashem","sequence":"additional","affiliation":[{"name":"Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Suez Canal University, 4.5 Ring Road, Ismailia 41522, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hatim A.","family":"Al-Abbadi","sequence":"additional","affiliation":[{"name":"University Hospital, Faculty of Medicine, King Abdulaziz University, Jeddah 80212, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7471-3148","authenticated-orcid":false,"given":"Elsayed","family":"Metwally","sequence":"additional","affiliation":[{"name":"Department of Cytology and Histology, Faculty of Veterinary Medicine, Suez Canal University, 4.5 Ring Road, Ismailia 41522, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"G\u00f3rnicki, T., Lambrinow, J., Golkar-Narenji, A., Data, K., Domaga\u0142a, D., Niebora, J., Farzaneh, M., Mozdziak, P., Zabel, M., and Antosik, P. 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