{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T09:28:24Z","timestamp":1787045304352,"version":"build-2736575974"},"reference-count":46,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004410","name":"Scientific and Technological Research Council of Turkey","doi-asserted-by":"publisher","award":["122C228"],"award-info":[{"award-number":["122C228"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004410","name":"Scientific and Technological Research Council of Turkey","doi-asserted-by":"publisher","award":["124C162"],"award-info":[{"award-number":["124C162"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]},{"award":["122C228"],"award-info":[{"award-number":["122C228"]}],"id":[{"id":"https:\/\/ror.org\/04w9kkr77","id-type":"ROR","asserted-by":"publisher"}]},{"award":["124C162"],"award-info":[{"award-number":["124C162"]}],"id":[{"id":"https:\/\/ror.org\/04w9kkr77","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomimetics"],"abstract":"<jats:p>Prostate cancer remains a major clinical challenge, partly due to tumour\u2013immune interactions that contribute to immune evasion and therapeutic resistance. The CD47\u2013SIRP\u03b1 axis is a key macrophage-associated immune recognition pathway; and materials-oriented platforms that allow preliminary investigation of tumour\u2013macrophage interaction patterns remain valuable. Here, we report an optically traceable biohybrid nanoplatform based on CD47 aptamer-functionalized fluorescent carboxyl-functional MMES-POSS hybrid nanoparticles. The nanoparticles were synthesized within 5 min using UV-induced free-radical emulsion polymerization in an ethanol\u2013water system and exhibited spherical morphology, SEM-derived dry-state mean diameters below 100 nm, negative surface charge, and retained fluorescence due to RITC encapsulation within the crosslinked hybrid matrix; however, DLS measurements revealed pronounced aggregation and polydispersity in aqueous dispersion. SEM and EDX provided complementary evidence of nanoparticle morphology and elemental composition, whereas zeta potential and Nanodrop measurements provided evidence consistent with surface functionalization, and FTIR confirmed retention of the core POSS, carbonyl, and RITC-associated chemistry. In a PC-3\/THP-1 macrophage co-culture model, Annexin V\u2013FITC\/PI flow cytometry showed a concentration-dependent redistribution of cell populations, with the most pronounced early apoptotic enrichment observed at 2 \u00b5g\/mL. However, pairwise exploratory comparisons among concentrations did not reach statistical significance, and this is discussed as a limitation of the present exploratory dose\u2013response characterization. These findings indicate preliminary interaction-associated response patterns rather than definitive receptor-specific targeting or therapeutic CD47\u2013SIRP\u03b1 blockade. Overall, this modular POSS-based biohybrid interface provides a materials-oriented platform for probing prostate cancer\u2013macrophage interaction profiles and guiding future mechanistic validation studies.<\/jats:p>","DOI":"10.3390\/biomimetics11080588","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T08:08:41Z","timestamp":1787040521000},"page":"588","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["CD47 Aptamer-Decorated Fluorescent POSS Hybrid Nanoparticles as a Biohybrid Interface for Probing Prostate Cancer\u2013Macrophage Interactions"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9004-0617","authenticated-orcid":false,"given":"Sumeyye","family":"Altunok","sequence":"first","affiliation":[{"name":"Medical School, Faculty of Medicine, At\u0131l\u0131m University, 06830 Ankara, T\u00fcrkiye"},{"name":"Department of Neurology, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2586-6770","authenticated-orcid":false,"given":"Gunes","family":"Kibar","sequence":"additional","affiliation":[{"name":"Medical School, Faculty of Medicine, At\u0131l\u0131m University, 06830 Ankara, T\u00fcrkiye"},{"name":"Micro Nano Particles (MNP) Research Group, Department of Materials Science and Engineering, Adana Alparslan T\u00fcrke\u015f Science and Technology University, 01250 Adana, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma","family":"Aylaz","sequence":"additional","affiliation":[{"name":"Medical School, Faculty of Medicine, At\u0131l\u0131m University, 06830 Ankara, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6623-8958","authenticated-orcid":false,"given":"Dide Su","family":"Demirel","sequence":"additional","affiliation":[{"name":"Medical School, Faculty of Medicine, At\u0131l\u0131m University, 06830 Ankara, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7659-5990","authenticated-orcid":false,"given":"Veli Cengiz","family":"Ozalp","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Istanbul Atlas University, 34408 Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.eururo.2024.11.013","article-title":"Recent Patterns and Trends in Global Prostate Cancer Incidence and Mortality: An Update","volume":"87","author":"Schafer","year":"2025","journal-title":"Eur. Urol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e99010018","DOI":"10.14440\/jbm.2024.0019","article-title":"Side Effects of Prostate Cancer Therapies and Potential Management","volume":"11","author":"Xiao","year":"2024","journal-title":"J. Biol. Methods"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"104384","DOI":"10.1016\/j.drudis.2025.104384","article-title":"A Review of the Efficacy of Prostate Cancer Therapies against Castration-Resistant Prostate Cancer","volume":"30","author":"Zhang","year":"2025","journal-title":"Drug Discov. Today"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Posdzich, P., Darr, C., Hilser, T., Wahl, M., Herrmann, K., Hadaschik, B., and Gr\u00fcnwald, V. (2023). Metastatic Prostate Cancer\u2014A Review of Current Treatment Options and Promising New Approaches. Cancers, 15.","DOI":"10.3390\/cancers15020461"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"101826","DOI":"10.1016\/j.mtbio.2025.101826","article-title":"Enhancing Tumor Immunotherapy with Smart Nanoparticles for Reprogramming Macrophages and Blocking the CD47\/Sirp\u03b1 Pathway","volume":"32","author":"Hussain","year":"2025","journal-title":"Mater. Today Bio"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"He, M., Cao, Y., Chi, C., Zhao, J., Chong, E., Chin, K.X.C., Tan, N.Z.V., Dmitry, K., Yang, G., and Yang, X. (2023). Unleashing Novel Horizons in Advanced Prostate Cancer Treatment: Investigating the Potential of Prostate Specific Membrane Antigen-Targeted Nanomedicine-Based Combination Therapy. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1265751"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"5183","DOI":"10.7150\/thno.85419","article-title":"Aptamer-Engineered (Nano)Materials for Theranostic Applications","volume":"13","author":"Rabiee","year":"2023","journal-title":"Theranostics"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1186\/s11671-021-03613-z","article-title":"Advances and Challenges of Fluorescent Nanomaterials for Synthesis and Biomedical Applications","volume":"16","author":"Xiao","year":"2021","journal-title":"Nanoscale Res. Lett."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"bpaf046","DOI":"10.1093\/biomethods\/bpaf046","article-title":"Advances and Future Directions of Aptamer-Functionalized Nanoparticles for Point-of-Care Diseases Diagnosis","volume":"10","author":"Fasogbon","year":"2025","journal-title":"Biol. Methods Protoc."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1002\/cnma.201800310","article-title":"Fluorescent Inorganic-Organic Hybrid Nanoparticles","volume":"5","author":"Neumeier","year":"2019","journal-title":"ChemNanoMat"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3375","DOI":"10.32604\/or.2025.071708","article-title":"CD47-Targeted Therapy in Cancer Immunotherapy: At a Crossroads of Promise and Challenge","volume":"33","author":"Guo","year":"2025","journal-title":"Oncol. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.coi.2012.01.010","article-title":"The CD47\u2013SIRP\u03b1 Pathway in Cancer Immune Evasion and Potential Therapeutic Implications","volume":"24","author":"Chao","year":"2012","journal-title":"Curr. Opin. Immunol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1016\/j.immuni.2020.04.011","article-title":"The CD47-SIRP\u03b1 Immune Checkpoint","volume":"52","author":"Logtenberg","year":"2020","journal-title":"Immunity"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"100070","DOI":"10.1016\/j.iotech.2022.100070","article-title":"CD47\u2013SIRP\u03b1-Targeted Therapeutics: Status and Prospects","volume":"13","author":"Maute","year":"2022","journal-title":"Immuno-Oncol. Technol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Jiang, C., Sun, H., Jiang, Z., Tian, W., Cang, S., and Yu, J. (2024). Targeting the CD47\/SIRP\u03b1 Pathway in Malignancies: Recent Progress, Difficulties and Future Perspectives. Front. Oncol., 14.","DOI":"10.3389\/fonc.2024.1378647"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Pei, M., Dai, X.-D., Jiang, X.-F., Yuan, C.-Y., Tan, J., He, K.-F., Liu, G.-J., Zhu, J.-D., Li, H.-H., and Pan, A.-L. (2025). A CD47 Antibody with Minimized Erythrocyte and Thrombocyte Toxicities. Front. Oncol., 15.","DOI":"10.3389\/fonc.2025.1686180"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Xu, Y., Jiang, P., Xu, Z., and Ye, H. (2024). Opportunities and Challenges for Anti-CD47 Antibodies in Hematological Malignancies. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1348852"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1007\/s11523-025-01130-y","article-title":"A Phase 1b\/2 Study of the Anti-CD47 Antibody Magrolimab with Cetuximab in Patients with Colorectal Cancer and Other Solid Tumors","volume":"20","author":"Eng","year":"2025","journal-title":"Target. Oncol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.2174\/1568026615666150413154233","article-title":"Aptamers: Molecular Tools for Medical Diagnosis","volume":"15","author":"Ozalp","year":"2015","journal-title":"Curr. Top. Med. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e00134","DOI":"10.1002\/adtp.202500134","article-title":"Enhanced Doxorubicin Cytotoxicity on Breast Cancer Spheroids by Aptamer Targeted Co-Delivery with Hyaluronidase","volume":"8","author":"Kavruk","year":"2025","journal-title":"Adv. Ther."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Liao, H., and Niu, C. (2022). Role of CD47-SIRP\u03b1 Checkpoint in Nanomedicine-Based Anti-Cancer Treatment. Front. Bioeng. Biotechnol., 10.","DOI":"10.3389\/fbioe.2022.887463"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"100139","DOI":"10.1016\/j.medidd.2022.100139","article-title":"CD47-SIRP\u03b1 Axis in Cancer Therapy: Precise Delivery of CD47-Targeted Therapeutics and Design of Anti-Phagocytic Drug Delivery Systems","volume":"15","author":"Zhu","year":"2022","journal-title":"Med. Drug Discov."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Li, S., Chen, Y., Ma, R., Du, Y., and Han, B. (2023). Cationic Lipid-Assisted Nanoparticles for Simultaneous Delivery of CD47 siRNA and R848 to Promote Antitumor Immune Responses. Front. Pharmacol., 14.","DOI":"10.3389\/fphar.2023.1142374"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4909","DOI":"10.1038\/s41467-020-18626-y","article-title":"Hybrid Cellular Membrane Nanovesicles Amplify Macrophage Immune Responses against Cancer Recurrence and Metastasis","volume":"11","author":"Rao","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1021\/acs.bioconjchem.2c00019","article-title":"Suppressing or Enhancing Macrophage Engulfment through the Use of CD47 and Related Peptides","volume":"33","author":"Jalil","year":"2022","journal-title":"Bioconjug. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"106810","DOI":"10.1016\/j.jddst.2025.106810","article-title":"Nanoengineered Oncolytic Virus-Mimicking Dendrimer to Synergize with Interference RNA to Induce Immunogenic Cell Death and up Regulation of \u201cFind-Me\u201d Signals","volume":"107","author":"Dharshini","year":"2025","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"rgaf127","DOI":"10.1093\/jpp\/rgaf127","article-title":"Engineering Stimuli-Responsive Nanocarriers for CRISPR\/Cas9 Genome Editing: Next-Generation Cancer Therapeutics","volume":"78","author":"Mehnath","year":"2026","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"127061","DOI":"10.1016\/j.matchemphys.2022.127061","article-title":"Antibiofilm and Enhanced Antibiotic Delivery by Halloysite Nanotubes Architected Dental Implant against Periodontitis","volume":"295","author":"Mehnath","year":"2023","journal-title":"Mater. Chem. Phys."},{"key":"ref_29","first-page":"100582","article-title":"pH\/NIR Dual-Responsive Implantable Patch for Combined Photothermal and Chemotherapy of Tumors","volume":"11","author":"Sun","year":"2026","journal-title":"Int. J. Pharm. X"},{"key":"ref_30","first-page":"100594","article-title":"Cyano-Modulated Zn(II)-Schiff Base Complexes for Lysosome-Targeted Fluorescence Imaging and pH-Responsive Camptothecin Delivery","volume":"12","author":"Zhang","year":"2026","journal-title":"Int. J. Pharm. X"},{"key":"ref_31","first-page":"5574","article-title":"Photoresponsive Carbon Dot Hydrogel for miR-155-Driven Immune Remodeling in Breast Cancer","volume":"9","author":"Dharshini","year":"2026","journal-title":"ACS Appl. Bio Mater."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.3390\/ph4081137","article-title":"Aptamer-Gated Nanoparticles for Smart Drug Delivery","volume":"4","author":"Ozalp","year":"2011","journal-title":"Pharmaceuticals"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2401762","DOI":"10.1002\/smll.202401762","article-title":"Recent Biomedical Applications of Functional Materials Based on Polyhedral Oligomeric Silsesquioxane (POSS)","volume":"20","author":"Chang","year":"2024","journal-title":"Small"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Ding, S., Zhao, S., Gan, X., Sun, A., Xia, Y., and Liu, Y. (2022). Design of Fluorescent Hybrid Materials Based on POSS for Sensing Applications. Molecules, 27.","DOI":"10.3390\/molecules27103137"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"49241","DOI":"10.1002\/app.49241","article-title":"Spherical Shape Poly(M-POSS) Micro\/Nano Hybrid Latex Particles: One-Step Synthesis and Characterization","volume":"137","author":"Kibar","year":"2020","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1007\/s10904-023-02540-z","article-title":"Effect of Crosslinking Agent on Mesoporous Spherical POSS Hybrid Particles: Synthesis, Characterization and Thermal Stability","volume":"33","author":"Kibar","year":"2023","journal-title":"J. Inorg. Organomet. Polym. Mater."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/s00604-024-06186-7","article-title":"Biosensor for ATP Detection via Aptamer-Modified PDA@POSS Nanoparticles Synthesized in a Microfluidic Reactor","volume":"191","author":"Kibar","year":"2024","journal-title":"Microchim. Acta"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3969","DOI":"10.1080\/07391102.2023.2217511","article-title":"Design Single-Stranded DNA Aptamer of Cluster of Differentiation 47 Protein by Stochastic Tunnelling-Basin Hopping-Discrete Molecular Dynamics Method","volume":"42","author":"Yang","year":"2024","journal-title":"J. Biomol. Struct. Dyn."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"25505","DOI":"10.1038\/s41598-025-10858-6","article-title":"M2-like Macrophages Derived from THP-1 Cells Promote Myofibroblast Differentiation of Synovial Fibroblasts in Association with the TGF-\u03921\/SMAD2\/3 Signaling Pathway","volume":"15","author":"Tung","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"15330338251356548","DOI":"10.1177\/15330338251356548","article-title":"Novel Anticancer Triple Formula Based on Aptamer-Conjugated PEGylated Nanoliposomes","volume":"24","author":"Nsairat","year":"2025","journal-title":"Technol. Cancer Res. Treat."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1678","DOI":"10.1007\/s12010-024-05091-2","article-title":"Comparative Analysis of Aptamer-Conjugated Chemical and Green Synthesized Gold Nanoparticles for Targeted Therapy in MCF-7 Cancer Cells","volume":"197","author":"Helal","year":"2025","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/s13014-021-01751-3","article-title":"Radiosensitization of Breast Cancer Cells Using AS1411 Aptamer-Conjugated Gold Nanoparticles","volume":"16","author":"Mehrnia","year":"2021","journal-title":"Radiat. Oncol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"pdb.prot087288","DOI":"10.1101\/pdb.prot087288","article-title":"Quantitation of Apoptosis and Necrosis by Annexin V Binding, Propidium Iodide Uptake, and Flow Cytometry","volume":"2016","author":"Crowley","year":"2016","journal-title":"Cold Spring Harb. Protoc."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"124799","DOI":"10.1016\/j.ijpharm.2024.124799","article-title":"Effects of Nanoparticle Size, Shape, and Zeta Potential on Drug Delivery","volume":"666","author":"Kaplan","year":"2024","journal-title":"Int. J. Pharm."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"15083","DOI":"10.1021\/acsnano.0c05091","article-title":"Multivalent, Soluble Nano-Self Peptides Increase Phagocytosis of Antibody-Opsonized Targets While Suppressing \u201cSelf\u201d Signaling","volume":"14","author":"Jalil","year":"2020","journal-title":"ACS Nano"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2051","DOI":"10.1016\/j.ccell.2023.10.007","article-title":"The Antitumor Activities of Anti-CD47 Antibodies Require Fc-Fc\u03b3R Interactions","volume":"41","author":"Osorio","year":"2023","journal-title":"Cancer Cell"}],"container-title":["Biomimetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2313-7673\/11\/8\/588\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T08:46:05Z","timestamp":1787042765000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2313-7673\/11\/8\/588"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,18]]},"references-count":46,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["biomimetics11080588"],"URL":"https:\/\/doi.org\/10.3390\/biomimetics11080588","relation":{},"ISSN":["2313-7673"],"issn-type":[{"value":"2313-7673","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,18]]}}}