{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T01:26:10Z","timestamp":1777598770871,"version":"3.51.4"},"reference-count":67,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T00:00:00Z","timestamp":1669680000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Office of Research and Innovation"},{"name":"Office of Research for Undergraduates"},{"name":"two Technology Fee awards at Miami University"},{"name":"Ohio Board of Regents and an Ohio Third Frontier Program award titled \u201cOhio Research Scholars"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomimetics"],"abstract":"<jats:p>Nonunion bone fractures can impact the quality of life and represent a major economic burden. Scaffold-based tissue engineering has shown promise as an alternative to bone grafting. Achieving desirable bone reconstruction requires appropriate surface properties, together with optimizing the internal architecture of 3D scaffolds. This study presents the surface modification of poly(lactic-co-glycolic acid) (PLGA), collagen, and PLGA-collagen via an argon plasma treatment. Argon plasma can modify the surface chemistry and topography of biomaterials and improve in vivo integration. Solvent-cast films were prepared using 1,1,1,3,3,3-hexafluoro-2-propanol and characterized via differential scanning calorimetry, thermogravimetric analysis, contact angle measurement, and critical surface tension analysis. For PLGA films, the water contact angle dropped from 70\u00b0 to 42\u00b0, whereas the diiodomethane contact angle reduced from 53\u00b0 to 32\u00b0 after the plasma treatment. A set of PLGA-collagen formulations were loaded with nanohydroxyapatite (nHA) and polyethylene glycol (PEG) to enhance their osteoconductivity and hydrophilicity. Then, 3D scaffolds were fabricated using a 3D Bioplotter and characterized via Fourier-transform infrared (FTIR) spectroscopy. A bicinchoninic acid assay (BCA) was used to compare the protein release from the untreated and plasma-treated scaffolds into phosphate-buffered saline (PBS). The plasma-treated scaffolds had a lower protein release, and the difference compared to the untreated scaffolds was statistically significant.<\/jats:p>","DOI":"10.3390\/biomimetics7040218","type":"journal-article","created":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T02:53:29Z","timestamp":1669690409000},"page":"218","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The Effect of Argon Plasma Surface Treatment on Poly(lactic-co-glycolic acid)\/Collagen-Based Biomaterials for Bone Tissue Engineering"],"prefix":"10.3390","volume":"7","author":[{"given":"Phat T.","family":"Vu","sequence":"first","affiliation":[{"name":"Department of Chemical, Paper and Biomedical Engineering, College of Engineering and Computing, Miami University, Oxford, OH 45056, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jackson P.","family":"Conroy","sequence":"additional","affiliation":[{"name":"Department of Chemical, Paper and Biomedical Engineering, College of Engineering and Computing, Miami University, Oxford, OH 45056, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9096-3147","authenticated-orcid":false,"given":"Amy M.","family":"Yousefi","sequence":"additional","affiliation":[{"name":"Department of Chemical, Paper and Biomedical Engineering, College of Engineering and Computing, Miami University, Oxford, OH 45056, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1615\/CritRevBiomedEng.v40.i5.10","article-title":"Bone tissue engineering: Recent advances and challenges","volume":"40","author":"Amini","year":"2012","journal-title":"Crit. Rev. Biomed. Eng."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.biomaterials.2018.07.017","article-title":"Bone regeneration strategies: Engineered scaffolds, bioactive molecules and stem cells current stage and future perspectives","volume":"180","author":"Bolander","year":"2018","journal-title":"Biomaterials"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.addr.2015.03.013","article-title":"Biomaterial strategies for engineering implants for enhanced osseointegration and bone repair","volume":"94","author":"Agarwal","year":"2015","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"8090","DOI":"10.1039\/D1BM00840D","article-title":"The role of biomaterials and scaffolds in immune responses in regenerative medicine: Macrophage phenotype modulation by biomaterial properties and scaffold architectures","volume":"9","author":"Antmen","year":"2021","journal-title":"Biomater. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1080\/09205063.2021.2019366","article-title":"Biomimetic sponge using duck\u2019s feet derived collagen and hydroxyapatite to promote bone regeneration","volume":"33","author":"Song","year":"2022","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Binlateh, T., Thammanichanon, P., Rittipakorn, P., Thinsathid, N., and Jitprasertwong, P. (2022). Collagen-Based Biomaterials in Periodontal Regeneration: Current Applications and Future Perspectives of Plant-Based Collagen. Biomimetics, 7.","DOI":"10.3390\/biomimetics7020034"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2445","DOI":"10.1007\/s10856-014-5240-2","article-title":"Bone substitutes in orthopaedic surgery: From basic science to clinical practice","volume":"25","author":"Campana","year":"2014","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1992","DOI":"10.1097\/SCS.0000000000001918","article-title":"Optimizing Collagen Scaffolds for Bone Engineering: Effects of Cross-linking and Mineral Content on Structural Contraction and Osteogenesis","volume":"26","author":"Lee","year":"2015","journal-title":"J. Craniofac. Surg."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"110480","DOI":"10.1016\/j.msec.2019.110480","article-title":"Porous calcium phosphate-collagen composite microspheres for effective growth factor delivery and bone tissue regeneration","volume":"109","author":"Seong","year":"2020","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.actbio.2021.03.067","article-title":"Recent advances in PLGA-based biomaterials for bone tissue regeneration","volume":"127","author":"Jin","year":"2021","journal-title":"Acta Biomater."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2607","DOI":"10.1016\/S0142-9612(01)00400-8","article-title":"Enhanced cell affinity of poly (D,L-lactide) by combining plasma treatment with collagen anchorage","volume":"23","author":"Yang","year":"2002","journal-title":"Biomaterials"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4777","DOI":"10.1016\/j.biomaterials.2003.11.051","article-title":"Characterization of surface property of poly(lactide-co-glycolide) after oxygen plasma treatment","volume":"25","author":"Wan","year":"2004","journal-title":"Biomaterials"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5913","DOI":"10.1007\/s10853-015-9152-4","article-title":"A review of low-temperature plasma treatment of textile materials","volume":"50","author":"Jelil","year":"2015","journal-title":"J. Mater. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1002\/ppap.201100126","article-title":"Surface Modification of Biopolymers by Argon Plasma and Thermal Treatment","volume":"9","author":"Sajdl","year":"2012","journal-title":"Plasma Process. Polym."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"6123","DOI":"10.2147\/IJN.S167637","article-title":"Argon plasma improves the tissue integration and angiogenesis of subcutaneous implants by modifying surface chemistry and topography","volume":"13","author":"Griffin","year":"2018","journal-title":"Int. J. Nanomed."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Mozaffari, A., Parvinzadeh Gashti, M., Mirjalili, M., and Parsania, M. (2021). Argon and Argon-Oxygen Plasma Surface Modification of Gelatin Nanofibers for Tissue Engineering Applications. Membranes, 11.","DOI":"10.3390\/membranes11010031"},{"key":"ref_17","unstructured":"Hollander, A.P., and Hatton, P.V. (2004). Production and Surface Modification of Polylactide-Based Polymeric Scaffolds for Soft-Tissue Engineering. Biopolymer Methods in Tissue Engineering, Humana Press."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1021\/ed077p63","article-title":"Contact Angle Goniometry as a Tool for Surface Tension Measurements of Solids, Using Zisman Plot Method. A Physical Chemistry Experiment","volume":"77","author":"Kabza","year":"2000","journal-title":"J. Chem. Educ."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.apsusc.2017.01.068","article-title":"Some remarks on the solid surface tension determination from contact angle measurements","volume":"405","author":"Zdziennicka","year":"2017","journal-title":"Appl. Surf. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"015023","DOI":"10.1088\/1758-5090\/9\/1\/015023","article-title":"Validation of scaffold design optimization in bone tissue engineering: Finite element modeling versus designed experiments","volume":"9","author":"Uth","year":"2017","journal-title":"Biofabrication"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1002\/1097-4636(200110)57:1<8::AID-JBM1135>3.0.CO;2-H","article-title":"Poly(DL-lactic-co-glycolic acid) sponge hybridized with collagen microsponges and deposited apatite particulates","volume":"57","author":"Chen","year":"2001","journal-title":"J. Biomed. Mater. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1023\/B:ABME.0000017544.36001.8e","article-title":"Polymeric scaffolds for bone tissue engineering","volume":"32","author":"Liu","year":"2004","journal-title":"Ann. Biomed. Eng."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Hatakeyama, W., Taira, M., Sawada, T., Hoshi, M., Hachinohe, Y., Sato, H., Takafuji, K., Kihara, H., Takemoto, S., and Kondo, H. (2022). Bone Regeneration of Critical-Size Calvarial Defects in Rats Using Highly Pressed Nano-Apatite\/Collagen Composites. Materials, 15.","DOI":"10.3390\/ma15093376"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1016\/j.biomaterials.2005.08.016","article-title":"Poly(lactide-co-glycolide)\/hydroxyapatite composite scaffolds for bone tissue engineering","volume":"27","author":"Kim","year":"2006","journal-title":"Biomaterials"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Baino, F., and Yamaguchi, S. (2020). The Use of Simulated Body Fluid (SBF) for Assessing Materials Bioactivity in the Context of Tissue Engineering: Review and Challenges. Biomimetics, 5.","DOI":"10.3390\/biomimetics5040057"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Shaikh, M.S., Zafar, M.S., and Alnazzawi, A. (2021). Comparing Nanohydroxyapatite Graft and Other Bone Grafts in the Repair of Periodontal Infrabony Lesions: A Systematic Review and Meta-Analysis. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222112021"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/s00068-004-1393-x","article-title":"Calcium Phosphate-Based Bone Substitutes","volume":"30","author":"Schnettler","year":"2004","journal-title":"Eur. J. Trauma"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.clay.2018.11.029","article-title":"Enhancing hydrophilicity and permeation flux of chitosan\/kaolin composite membranes by using polyethylene glycol as porogen","volume":"168","author":"Rekik","year":"2019","journal-title":"Appl. Clay Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1089\/ten.tea.2006.0418","article-title":"Design and fabrication of 3D porous scaffolds to facilitate cell-based gene therapy","volume":"14","author":"Eliopoulos","year":"2008","journal-title":"Tissue Eng. Part A"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.biomaterials.2010.08.094","article-title":"Hierarchical scaffold design for mesenchymal stem cell-based gene therapy of hemophilia B","volume":"32","author":"Coutu","year":"2011","journal-title":"Biomaterials"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/0003-2697(85)90442-7","article-title":"Measurement of protein using bicinchoninic acid","volume":"150","author":"Smith","year":"1985","journal-title":"Anal. Biochem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.colsurfb.2017.12.030","article-title":"Non-equilibrium atmospheric pressure plasma as innovative method to crosslink and enhance mucoadhesion of econazole-loaded gelatin films for buccal drug delivery","volume":"163","author":"Dolci","year":"2018","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0142-9612(00)00136-8","article-title":"Optimizing the sterilization of PLGA scaffolds for use in tissue engineering","volume":"22","author":"Holy","year":"2001","journal-title":"Biomaterials"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Walker, J.M. (2009). The Bicinchoninic Acid (BCA) Assay for Protein Quantitation BT\u2014The Protein Protocols Handbook, Humana Press.","DOI":"10.1007\/978-1-59745-198-7_3"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"271","DOI":"10.2174\/1874120701004010271","article-title":"Competitive Binding to Cuprous Ions of Protein and BCA in the Bicinchoninic Acid Protein Assay","volume":"4","author":"Huang","year":"2010","journal-title":"Open Biomed. Eng. J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1366\/000370209788701044","article-title":"Attenuated total internal reflection Fourier transform infrared spectroscopy: A quantitative approach for kidney stone analysis","volume":"63","author":"Haas","year":"2009","journal-title":"Appl. Spectrosc."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1016\/j.msec.2017.06.012","article-title":"Comparison of thermal properties of fish collagen and bovine collagen in the temperature range 298\u2013670 K","volume":"80","author":"Kubisz","year":"2017","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Lu, B., Lv, X., and Le, Y. (2019). Chitosan-Modified PLGA Nanoparticles for Control-Released Drug Delivery. Polymers, 11.","DOI":"10.3390\/polym11020304"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.micron.2010.09.010","article-title":"Collagen type I amide I band infrared spectroscopy","volume":"42","author":"Mello","year":"2011","journal-title":"Micron"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.saa.2011.07.036","article-title":"FTIR spectroscopic, thermal and XRD characterization of hydroxyapatite from new natural sources","volume":"83","author":"Shaltout","year":"2011","journal-title":"Spectrochim. Acta Part A Mol. Biomol. Spectrosc."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2845","DOI":"10.3390\/molecules15042845","article-title":"Cell proliferation of HaCaT keratinocytes on collagen films modified by argon plasma treatment","volume":"15","author":"Asadinezhad","year":"2010","journal-title":"Molecules"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Donate, R., Alem\u00e1n-Dom\u00ednguez, M.E., and Monz\u00f3n, M. (2021). On the Effectiveness of Oxygen Plasma and Alkali Surface Treatments to Modify the Properties of Polylactic Acid Scaffolds. Polymers, 13.","DOI":"10.3390\/polym13101643"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1016\/j.msec.2008.07.026","article-title":"Plasma surface treatments of poly(l-lactic acid) (PLLA) and poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV)","volume":"29","author":"Ferreira","year":"2009","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1089\/ten.tea.2011.0725","article-title":"Plasma surface chemical treatment of electrospun poly(L-lactide) microfibrous scaffolds for enhanced cell adhesion, growth, and infiltration","volume":"19","author":"Cheng","year":"2013","journal-title":"Tissue Eng. Part A"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.surfcoat.2013.02.042","article-title":"Surface modification of polymers by plasma treatments for the enhancement of biocompatibility and controlled drug release","volume":"233","author":"Yoshida","year":"2013","journal-title":"Surf. Coat. Technol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.radphyschem.2007.05.013","article-title":"Plasma surface modification of poly (l-lactic acid) and poly (lactic-co-glycolic acid) films for improvement of nerve cells adhesion","volume":"77","author":"Khorasani","year":"2008","journal-title":"Radiat. Phys. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1002\/term.2859","article-title":"Tuning the biomimetic behavior of scaffolds for regenerative medicine through surface modifications","volume":"13","author":"Richbourg","year":"2019","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1007\/s13770-017-0075-9","article-title":"Electrospun Collagen Nanofibers and Their Applications in Skin Tissue Engineering","volume":"14","author":"Law","year":"2017","journal-title":"Tissue Eng. Regen. Med."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"110085","DOI":"10.1016\/j.msec.2019.110085","article-title":"Argon plasma modification promotes adipose derived stem cells osteogenic and chondrogenic differentiation on nanocomposite polyurethane scaffolds; implications for skeletal tissue engineering","volume":"105","author":"Griffin","year":"2019","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Krok-Borkowicz, M., Reczy\u0144ska, K., Rumian, \u0141., Menaszek, E., Orzelski, M., Malisz, P., Silmanowicz, P., Dobrzy\u0144ski, P., and Pamu\u0142a, E. (2020). Surface-Modified Poly(l-lactide-co-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects-In Vivo Studies on Rabbits. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21207541"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1016\/S0142-9612(01)00317-9","article-title":"Adsorption and dissolution behavior of human plasma fibronectin on thermally and chemically modified titanium dioxide particles","volume":"23","author":"MacDonald","year":"2002","journal-title":"Biomaterials"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1177\/0022034516629119","article-title":"Plasma of Argon Affects the Earliest Biological Response of Different Implant Surfaces: An In Vitro Comparative Study","volume":"95","author":"Canullo","year":"2016","journal-title":"J. Dent. Res."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Carossa, M., Cavagnetto, D., Mancini, F., Mosca Balma, A., and Mussano, F. (2022). Plasma of Argon Treatment of the Implant Surface, Systematic Review of In Vitro Studies. Biomolecules, 12.","DOI":"10.3390\/biom12091219"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"41962","DOI":"10.1021\/acsami.8b14995","article-title":"Plasma Functionalization of Polycaprolactone Nanofibers Changes Protein Interactions with Cells, Resulting in Increased Cell Viability","volume":"10","author":"Asadian","year":"2018","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"136639","DOI":"10.1016\/j.cej.2022.136639","article-title":"Superhydrophilic 3D-printed scaffolds using conjugated bioresorbable nanocomposites for enhanced bone regeneration","volume":"445","author":"Dave","year":"2022","journal-title":"Chem. Eng. J."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Ayyoob, M., and Kim, Y.J. (2018). Effect of Chemical Composition Variant and Oxygen Plasma Treatments on the Wettability of PLGA Thin Films, Synthesized by Direct Copolycondensation. Polymers, 10.","DOI":"10.3390\/polym10101132"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1590\/1516-1439.045415","article-title":"Study of thermal degradation of PLGA, PLGA nanospheres and PLGA\/maghemite superparamagnetic nanospheres","volume":"18","author":"Silva","year":"2015","journal-title":"Mater. Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2293","DOI":"10.1016\/S1452-3981(23)14309-4","article-title":"Preparation and in vitro thermo-mechanical characterization of electrospun PLGA nanofibers for soft and hard tissue replacement","volume":"8","author":"Fouad","year":"2013","journal-title":"Int. J. Electrochem. Sci"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.bpj.2011.04.033","article-title":"Thermal denaturation studies of collagen by microthermal analysis and atomic force microscopy","volume":"101","author":"Bozec","year":"2011","journal-title":"Biophys. J."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1002\/cjce.23673","article-title":"Experimental methods in chemical engineering: Thermogravimetric analysis\u2014TGA","volume":"98","author":"Saadatkhah","year":"2020","journal-title":"Can. J. Chem. Eng."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1177\/088391159801300106","article-title":"Sterilization of Sutures by Low Temperature Argon Plasma","volume":"13","author":"Ayhan","year":"1998","journal-title":"J. Bioact. Compat. Polym."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1688","DOI":"10.1109\/TPS.2012.2190303","article-title":"Effect of Low-Temperature Plasma Jet on Thermal Stability and Physical Structure of Type I Collagen","volume":"40","author":"Samouillan","year":"2012","journal-title":"IEEE Trans. Plasma Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2009","DOI":"10.1002\/prot.25354","article-title":"Temperature stability of proteins: Analysis of irreversible denaturation using isothermal calorimetry","volume":"85","author":"Clarkson","year":"2017","journal-title":"Proteins"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/j.foodhyd.2006.07.007","article-title":"Denaturation and aggregation processes in thermal gelation of whey proteins resolved by differential scanning calorimetry","volume":"21","author":"Fitzsimons","year":"2007","journal-title":"Food Hydrocoll."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1702","DOI":"10.1021\/ac60131a045","article-title":"Reaction kinetics in differential thermal analysis","volume":"29","author":"Kissinger","year":"1957","journal-title":"Anal. Chem."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1023\/A:1013953316829","article-title":"Thermal and Rheological Behavior of Collagen. Chitosan blends","volume":"67","author":"Martins","year":"2002","journal-title":"J. Therm. Anal. Calorim."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1016\/j.eurpolymj.2006.01.007","article-title":"The investigation of argon plasma surface modification to polyethylene: Quantitative ATR-FTIR spectroscopic analysis","volume":"42","author":"Shi","year":"2006","journal-title":"Eur. Polym. J."}],"container-title":["Biomimetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2313-7673\/7\/4\/218\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:29:01Z","timestamp":1760146141000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2313-7673\/7\/4\/218"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,29]]},"references-count":67,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["biomimetics7040218"],"URL":"https:\/\/doi.org\/10.3390\/biomimetics7040218","relation":{},"ISSN":["2313-7673"],"issn-type":[{"value":"2313-7673","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,29]]}}}