{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:36:38Z","timestamp":1790868998394,"version":"4.1.0"},"reference-count":66,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,9,21]],"date-time":"2023-09-21T00:00:00Z","timestamp":1695254400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Higher Education of the Russian Federation","award":["FSWW-2023-0007"],"award-info":[{"award-number":["FSWW-2023-0007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomimetics"],"abstract":"<jats:p>A promising method for improving the functional properties of calcium-phosphate coatings is the incorporation of various antibacterial additives into their structure. The microbial contamination of a superficial wound is inevitable, even if the rules of asepsis and antisepsis are optimally applied. One of the main problems is that bacteria often become resistant to antibiotics over time. However, this does not apply to certain elements, chemical compounds and drugs with antimicrobial properties. In this study, the fabrication and properties of zinc-containing calcium-phosphate coatings that were formed via micro-arc oxidation from three different electrolyte solutions are investigated. The first electrolyte is based on calcium oxide, the second on hydroxyapatite and the third on calcium acetate. By adding zinc oxide to the three electrolyte solutions, antibacterial properties of the coatings are achieved. Although the same amount of zinc oxide has been added to each electrolyte solution, the zinc concentration in the coatings obtained vary greatly. Furthermore, this study investigates the morphology, structure and chemical composition of the coatings. The antibacterial properties of the zinc-containing coatings were tested toward three strains of bacteria\u2014Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa. Coatings of calcium acetate and zinc oxide contained the highest amount of zinc and displayed the highest zinc release. Moreover, coatings containing hydroxyapatite and zinc oxide show the highest antibacterial activity toward Pseudomonas aeruginosa, and coatings containing calcium acetate and zinc oxide show the highest antibacterial activities toward Staphylococcus aureus and methicillin-resistant Staphylococcus aureus.<\/jats:p>","DOI":"10.3390\/biomimetics8050444","type":"journal-article","created":{"date-parts":[[2023,9,21]],"date-time":"2023-09-21T04:53:05Z","timestamp":1695271985000},"page":"444","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Antibacterial Calcium Phosphate Coatings for Biomedical Applications Fabricated via Micro-Arc Oxidation"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0168-0952","authenticated-orcid":false,"given":"Anna I.","family":"Kozelskaya","sequence":"first","affiliation":[{"name":"Weinberg Research Center, School of Nuclear Science &Engineering, Tomsk Polytechnic University, 30, Lenin Avenue, 634050 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ksenia N.","family":"Verzunova","sequence":"additional","affiliation":[{"name":"Weinberg Research Center, School of Nuclear Science &Engineering, Tomsk Polytechnic University, 30, Lenin Avenue, 634050 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor O.","family":"Akimchenko","sequence":"additional","affiliation":[{"name":"Weinberg Research Center, School of Nuclear Science &Engineering, Tomsk Polytechnic University, 30, Lenin Avenue, 634050 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3107-7030","authenticated-orcid":false,"given":"Johannes","family":"Frueh","sequence":"additional","affiliation":[{"name":"Weinberg Research Center, School of Nuclear Science &Engineering, Tomsk Polytechnic University, 30, Lenin Avenue, 634050 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vsevolod I.","family":"Petrov","sequence":"additional","affiliation":[{"name":"Tomsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences, 10\/4, Akademicheskii Prospekt, 634055 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina B.","family":"Slepchenko","sequence":"additional","affiliation":[{"name":"Weinberg Research Center, School of Nuclear Science &Engineering, Tomsk Polytechnic University, 30, Lenin Avenue, 634050 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga V.","family":"Bakina","sequence":"additional","affiliation":[{"name":"Institute of Strength Physics and Materials Science of the Siberian Branch of the Russian Academy of Sciences, 2\/4, Akademicheskii Prospekt, 634055 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marat I.","family":"Lerner","sequence":"additional","affiliation":[{"name":"Institute of Strength Physics and Materials Science of the Siberian Branch of the Russian Academy of Sciences, 2\/4, Akademicheskii Prospekt, 634055 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonid K.","family":"Brizhan","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Institution \u00abMain Military Clinical Hospital Named after Academician N.N. 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Eng. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1080\/07388551.2020.1828810","article-title":"Strategies for antimicrobial peptide coatings on medical devices: A review and regulatory science perspective","volume":"41","author":"Cheng","year":"2021","journal-title":"Crit. Rev. Biotechnol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"14452","DOI":"10.1073\/pnas.1508520112","article-title":"Regenerative medicine: Current therapies and future directions","volume":"112","author":"Mao","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_4","first-page":"6","article-title":"Orthopedic implants and devices for bone fractures and defects: Past, present and perspective","volume":"1","author":"Kim","year":"2020","journal-title":"Eng. Regen."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1902\/jop.2015.150229","article-title":"Relationship Between Osteoporosis and Marginal Bone Loss in Osseointegrated Implants: A 2-Year Retrospective Study","volume":"87","year":"2016","journal-title":"J. Periodontol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4229","DOI":"10.1016\/j.actbio.2011.07.017","article-title":"Histological and three-dimensional evaluation of osseointegration to nanostructured calcium phosphate-coated implants","volume":"7","author":"Jimbo","year":"2011","journal-title":"Acta Biomater."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1615\/CritRevBiomedEng.v28.i56.10","article-title":"Hydroxyapatite and Their Use as Coatings in Dental Implants: A Review","volume":"28","author":"Ong","year":"2000","journal-title":"Crit. Rev. Biomed. Eng."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1002\/jbm.a.30130","article-title":"The effect of different titanium and hydroxyapatite-coated dental implant surfaces on phenotypic expression of human bone-derived cells","volume":"71A","author":"Knabe","year":"2004","journal-title":"J. Biomed. Mater. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1089\/sur.2017.020","article-title":"Optimum Operating Room Environment for the Prevention of Surgical Site Infections","volume":"18","author":"Gaines","year":"2017","journal-title":"Surg. Infect."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.jallcom.2017.12.366","article-title":"Study of the effect of ZnO nanoparticles addition to PEO coatings on pure titanium substrate: Microstructural analysis, antibacterial effect and corrosion behavior of coatings in Ringer\u2019s physiological solution","volume":"740","author":"Roknian","year":"2018","journal-title":"J. Alloys Compd."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Mozafarnia, H., Fattah-Alhosseini, A., Chaharmahali, R., Nouri, M., Keshavarz, M.K., and Kaseem, M. (2022). Corrosion, Wear, and Antibacterial Behaviors of Hydroxyapatite\/MgO Composite PEO Coatings on AZ31 Mg Alloy by Incorporation of TiO2 Nanoparticles. Coatings, 12.","DOI":"10.3390\/coatings12121967"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"111098","DOI":"10.1016\/j.msec.2020.111098","article-title":"Electrochemical modification of the Ti-15Mo alloy surface in solutions containing ZnO and Zn3(PO4)2 particles","volume":"115","author":"Rokosz","year":"2020","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Sasikumar, Y., Indira, K., and Rajendran, N. (2019). Surface Modification Methods for Titanium and Its Alloys and Their Corrosion Behavior in Biological Environment: A Review. J. Bio-Tribo-Corros., 5.","DOI":"10.1007\/s40735-019-0229-5"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1016\/j.apsusc.2010.08.003","article-title":"Synthesis and bactericidal ability of Ag\/TiO2 composite films deposited on titanium plate","volume":"257","author":"Mai","year":"2010","journal-title":"Appl. Surf. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.triboint.2010.10.031","article-title":"A comparative study of the tribological behaviour of PVD coatings on the Ti-6Al-4V alloy","volume":"44","author":"Martini","year":"2011","journal-title":"Tribol. Int."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/S0257-8972(97)00459-3","article-title":"CVD diamond coated titanium alloys for biomedical and aerospace applications","volume":"94\u201395","author":"Heinrich","year":"1997","journal-title":"Surf. Coat. Technol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1016\/S0142-9612(02)00573-2","article-title":"In vitro studies of plasma-sprayed hydroxyapatite\/Ti-6Al-4V composite coatings in simulated body fluid (SBF)","volume":"24","author":"Gu","year":"2003","journal-title":"Biomaterials"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1016\/j.actbio.2009.08.010","article-title":"Laser cladding of bioactive glass coatings","volume":"6","author":"Quintero","year":"2010","journal-title":"Acta Biomater."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2875","DOI":"10.1016\/j.actbio.2012.04.005","article-title":"A review of the application of anodization for the fabrication of nanotubes on metal implant surfaces","volume":"8","author":"Minagar","year":"2012","journal-title":"Acta Biomater."},{"key":"ref_20","unstructured":"Jelinek, M. (2013). Lasers for Medical Applications, Elsevier."},{"key":"ref_21","unstructured":"Sima, F., Ristoscu, C., Duta, L., Gallet, O., Anselme, K., and Mihailescu, I.N. (2016). Laser Surface Modification of Biomaterials, Elsevier."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"20587","DOI":"10.1016\/j.ceramint.2020.05.206","article-title":"On the enhanced antibacterial activity of plasma electrolytic oxidation (PEO) coatings that incorporate particles: A review","volume":"46","author":"Molaei","year":"2020","journal-title":"Ceram. Int."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Prosolov, K.A., Mitrichenko, D.V., Prosolov, A.B., Nikolaeva, O.O., Lastovka, V.V., Belyavskaya, O.A., Chebodaeva, V.A., Glukhov, I.A., Litvinova, L.S., and Shupletsova, V.V. (2021). Zn-Doped CaP-Based Coatings on Ti\u20136Al\u20134V and Ti\u20136Al\u20137Nb Alloys Prepared by Magnetron Sputtering: Controllable Biodegradation, Bacteriostatic, and Osteogenic Activities. Coatings, 11.","DOI":"10.3390\/coatings11070809"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Komarova, E.G., Sharkeev, Y.P., Sedelnikova, M.B., Prymak, O., Epple, M., Litvinova, L.S., Shupletsova, V.V., Malashchenko, V.V., Yurova, K.A., and Dzyuman, A.N. (2020). Zn- or Cu-containing CaP-Based Coatings Formed by Micro-Arc Oxidation on Titanium and Ti-40Nb Alloy: Part II\u2014Wettability and Biological Performance. Materials, 13.","DOI":"10.3390\/ma13194366"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1016\/j.msec.2016.10.079","article-title":"Incorporation of silver and strontium in hydroxyapatite coating on titanium surface for enhanced antibacterial and biological properties","volume":"71","author":"Geng","year":"2017","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3341","DOI":"10.1016\/j.biomaterials.2003.09.103","article-title":"Biomimetic apatite coatings on micro-arc oxidized titania","volume":"25","author":"Song","year":"2004","journal-title":"Biomaterials"},{"key":"ref_27","unstructured":"Jiang, B.L., and Wang, Y.M. (2010). Surface Engineering of Light Alloys, Elsevier."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"102403","DOI":"10.1016\/j.surfin.2022.102403","article-title":"Guar gum-driven high-energy plasma electrolytic oxidation for concurrent improvements in the electrochemical and catalytic properties of Ti-15 Zr alloy","volume":"34","author":"Kaseem","year":"2022","journal-title":"Surf. Interfaces"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1007\/s10717-018-0067-9","article-title":"Porous Functional Coatings by Microarc Oxidation","volume":"75","author":"Markov","year":"2018","journal-title":"Glas. Ceram."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Fattah-alhosseini, A., Chaharmahali, R., Rajabi, A., Babaei, K., and Kaseem, M. (2022). Performance of PEO\/Polymer Coatings on the Biodegradability, Antibacterial Effect and Biocompatibility of Mg-Based Materials. J. Funct. Biomater., 13.","DOI":"10.3390\/jfb13040267"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/j.surfcoat.2018.06.024","article-title":"Influence of electrolyte composition on microstructure and properties of coatings formed on pure Ti substrate by micro arc oxidation","volume":"349","author":"Yang","year":"2018","journal-title":"Surf. Coat. Technol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Shimabukuro, M. (2020). Antibacterial Property and Biocompatibility of Silver, Copper, and Zinc in Titanium Dioxide Layers Incorporated by One-Step Micro-Arc Oxidation: A Review. Antibiotics, 9.","DOI":"10.3390\/antibiotics9100716"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Fosca, M., Streza, A., Antoniac, I.V., Vadal\u00e0, G., and Rau, J.V. (2023). Ion-Doped Calcium Phosphate-Based Coatings with Antibacterial Properties. J. Funct. Biomater., 14.","DOI":"10.3390\/jfb14050250"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1016\/0306-3623(94)90364-6","article-title":"Cytotoxicity and accumulation of Hg, Ag, Cd, Cu, Pb and Zn in human peripheral T and B lymphocytes and monocytes In Vitro","volume":"25","author":"Steffensen","year":"1994","journal-title":"Gen. Pharmacol. Vasc. Syst."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Gammoh, N., and Rink, L. (2017). Zinc in Infection and Inflammation. Nutrients, 9.","DOI":"10.20944\/preprints201705.0176.v1"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1093\/advances\/nmz013","article-title":"The Role of Zinc in Antiviral Immunity","volume":"10","author":"Read","year":"2019","journal-title":"Adv. Nutr."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1111\/j.1574-6968.2007.01012.x","article-title":"Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms","volume":"279","author":"Jones","year":"2008","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.colsurfa.2014.05.057","article-title":"The contribution of zinc ions to the antimicrobial activity of zinc oxide","volume":"457","author":"Pasquet","year":"2014","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Villap\u00fan, V.M., Esat, F., Bull, S., Dover, L.G., and Gonz\u00e1lez, S. (2017). Tuning the Mechanical and Antimicrobial Performance of a Cu-Based Metallic Glass Composite through Cooling Rate Control and Annealing. Materials, 10.","DOI":"10.3390\/ma10050506"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Yang, W., Yu, S., Wang, L., Ma, X., Gao, W., Lan, N., Shao, W., and Chen, J. (2022). Microstructure and Properties in Simulated Seawater of Copper-Doped Micro-arc Coatings on TC4 Alloy. Coatings, 12.","DOI":"10.3390\/coatings12070883"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Salahshouri, F., Saebnoori, E., Borghei, S., Mossahebi-Mohammadi, M., Bakhsheshi-Rad, H.R., and Berto, F. (2022). Plasma Electrolytic Oxidation (PEO) Coating on \u03b3-TiAl Alloy: Investigation of Bioactivity and Corrosion Behavior in Simulated Body Fluid. Metals, 12.","DOI":"10.3390\/met12111866"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"125987","DOI":"10.1016\/j.surfcoat.2020.125987","article-title":"Characterization of metal oxide micro\/nanoparticles elaborated by plasma electrolytic oxidation of aluminium and zirconium alloys","volume":"397","author":"Martin","year":"2020","journal-title":"Surf. Coat. Technol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"112030","DOI":"10.1016\/j.msec.2021.112030","article-title":"A novel titanium implant surface modification by plasma electrolytic oxidation (PEO) preventing tendon adhesion","volume":"123","author":"Becerikli","year":"2021","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"S428","DOI":"10.1016\/j.surfcoat.2012.05.037","article-title":"Effect of Zn content on cytoactivity and bacteriostasis of micro-arc oxidation coatings on pure titanium","volume":"228","author":"Zhao","year":"2013","journal-title":"Surf. Coat. Technol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Toulabifard, A., Rahmati, M., Raeissi, K., Hakimizad, A., and Santamaria, M. (2020). The Effect of Electrolytic Solution Composition on the Structure, Corrosion, and Wear Resistance of PEO Coatings on AZ31 Magnesium Alloy. Coatings, 10.","DOI":"10.3390\/coatings10100937"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"126349","DOI":"10.1016\/j.surfcoat.2020.126349","article-title":"Role of the electrolyte composition in establishing plasma discharges and coating growth process during a micro-arc oxidation","volume":"402","author":"Wang","year":"2020","journal-title":"Surf. Coat. Technol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Dziaduszewska, M., Shimabukuro, M., Seramak, T., Zielinski, A., and Hanawa, T. (2020). Effects of Micro-Arc Oxidation Process Parameters on Characteristics of Calcium-Phosphate Containing Oxide Layers on the Selective Laser Melted Ti13Zr13Nb Alloy. Coatings, 10.","DOI":"10.3390\/coatings10080745"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Yan, H., Liu, W., Ma, Y., Wang, T., Wu, L., Yang, L., and Tang, S. (2023). Effects of Micro-arc Oxidation Process Parameters on Micro-structure and Properties of Al2O3 Coatings Prepared on Sintered 2024 Aluminum Alloy. J. Mater. Eng. Perform.","DOI":"10.1007\/s11665-023-08093-z"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"8746","DOI":"10.1016\/j.surfcoat.2006.11.023","article-title":"Electrolytic plasma technology: Science and engineering\u2014An overview","volume":"201","author":"Gupta","year":"2007","journal-title":"Surf. Coat. Technol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0257-8972(99)00441-7","article-title":"Plasma electrolysis for surface engineering","volume":"122","author":"Yerokhin","year":"1999","journal-title":"Surf. Coat. Technol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1134\/1.1522119","article-title":"Specific features of electrolytic-plasma quenching","volume":"47","author":"Tyurin","year":"2002","journal-title":"Tech. Phys."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"100735","DOI":"10.1016\/j.pmatsci.2020.100735","article-title":"Recent progress in surface modification of metals coated by plasma electrolytic oxidation: Principle, structure, and performance","volume":"117","author":"Kaseem","year":"2021","journal-title":"Prog. Mater. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Kozelskaya, A.I., Rutkowski, S., Frueh, J., Gogolev, A.S., Chistyakov, S.G., Gnedenkov, S.V., Sinebryukhov, S.L., Frueh, A., Egorkin, V.S., and Choynzonov, E.L. (2022). Surface Modification of Additively Fabricated Titanium-Based Implants by Means of Bioactive Micro-Arc Oxidation Coatings for Bone Replacement. J. Funct. Biomater., 13.","DOI":"10.3390\/jfb13040285"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Sharkeev, Y.P., Komarova, E.G., Chebodaeva, V.V., Sedelnikova, M.B., Zakharenko, A.M., Golokhvast, K.S., Litvinova, L.S., Khaziakhmatova, O.G., Malashchenko, V.V., and Yurova, K.A. (2021). Amorphous\u2013Crystalline Calcium Phosphate Coating Promotes In Vitro Growth of Tumor-Derived Jurkat T Cells Activated by Anti-CD2\/CD3\/CD28 Antibodies. Materials, 14.","DOI":"10.3390\/ma14133693"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Komarova, E.G., Sharkeev, Y.P., Sedelnikova, M.B., Prosolov, K.A., Khlusov, I.A., Prymak, O., and Epple, M. (2020). Zn- or Cu-Containing CaP-Based Coatings Formed by Micro-arc Oxidation on Titanium and Ti-40Nb Alloy: Part I\u2014Microstructure, Composition and Properties. Materials, 13.","DOI":"10.3390\/ma13184116"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S1003-6326(17)60014-1","article-title":"Structure and properties of micro-arc calcium phosphate coatings on pure titanium and Ti\u201340Nb alloy","volume":"27","author":"Sharkeev","year":"2017","journal-title":"Trans. Nonferrous Met. Soc. China"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1134\/S0036023622020048","article-title":"Calcium Phosphate Apatites: Wet Formation, Thermal Transformations, Terminology, and Identification","volume":"67","author":"Glazov","year":"2022","journal-title":"Russ. J. Inorg. Chem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/s10856-012-4787-z","article-title":"Stability of plasma electrolytic oxidation coating on titanium in artificial saliva","volume":"24","author":"Matykina","year":"2013","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2260","DOI":"10.1177\/0040517518790973","article-title":"UV protection and antimicrobial finish on cotton khadi fabric using a mixture of nanoparticles of zinc oxide and poly-hydroxy-amino methyl silicone","volume":"89","author":"Kar","year":"2019","journal-title":"Text. Res. J."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/s40820-015-0040-x","article-title":"Review on Zinc Oxide Nanoparticles: Antibacterial Activity and Toxicity Mechanism","volume":"7","author":"Sirelkhatim","year":"2015","journal-title":"Nano-Micro Lett."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"100839","DOI":"10.1016\/j.mtla.2020.100839","article-title":"Calcium ion mediated rapid wound healing by nano-ZnO doped calcium phosphate-chitosan-alginate biocomposites","volume":"13","author":"Rahman","year":"2020","journal-title":"Materialia"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"9395","DOI":"10.2147\/IJN.S216204","article-title":"Relationship Between Structure and Antimicrobial Activity Of Zinc Oxide Nanoparticles: An Overview","volume":"14","author":"Pietro","year":"2019","journal-title":"Int. J. Nanomed."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1186\/s11671-020-03418-6","article-title":"A Review on Enhancing the Antibacterial Activity of ZnO: Mechanisms and Microscopic Investigation","volume":"15","author":"Abebe","year":"2020","journal-title":"Nanoscale Res. Lett."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2658","DOI":"10.1038\/s41598-022-06657-y","article-title":"de M. R.; Lopes, P.R.M.; de Moraes, P.B.; Montagnolli, R.N.; Ferreira, H.; Bidoia, E.D. Antibacterial action and target mechanisms of zinc oxide nanoparticles against bacterial pathogens","volume":"12","author":"Mendes","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/0009-2797(89)90085-9","article-title":"Cytotoxicity of zinc in vitro","volume":"69","author":"Riley","year":"1989","journal-title":"Chem. Biol. Interact."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"8148","DOI":"10.1038\/s41598-022-12134-3","article-title":"ZnO size and shape effect on antibacterial activity and cytotoxicity profile","volume":"12","author":"Babayevska","year":"2022","journal-title":"Sci. Rep."}],"container-title":["Biomimetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2313-7673\/8\/5\/444\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:54:29Z","timestamp":1760129669000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2313-7673\/8\/5\/444"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,21]]},"references-count":66,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2023,9]]}},"alternative-id":["biomimetics8050444"],"URL":"https:\/\/doi.org\/10.3390\/biomimetics8050444","relation":{},"ISSN":["2313-7673"],"issn-type":[{"value":"2313-7673","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,21]]}}}