{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T16:14:53Z","timestamp":1790007293276,"version":"4.0.1"},"reference-count":52,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T00:00:00Z","timestamp":1731628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomimetics"],"abstract":"<jats:p>Anthocyanin is an important flavonoid with antioxidant, anticancer, and anti-inflammatory properties. This research investigates the anthocyanin content of Cavendish banana inflorescence, a by-product often discarded as agricultural waste. The study employs two drying methods, namely oven-drying and freeze-drying, followed by accelerated solvent extraction using acidified water and methanol. Liquid chromatography-mass spectroscopy (LC-MS) results confirm banana inflorescence as a rich source of anthocyanins. According to LC-MS analysis, freeze-dried banana inflorescence extracted in methanol at 80 \u00b0C exhibits the highest anthocyanin content (130.01 mg\/100 g). This sample also demonstrates superior characteristics, including a chroma value of 40.02 \u00b1 0.01, a redness value of 38.09 \u00b1 0.16, 18.46 \u00b1 0.02 \u00b0Brix, a total phenolic content of 42.5 \u00b1 1.00 mg\/g, expressed as gallic acid equivalents, and a total antioxidant activity of 71.33 \u00b1 0.08% when assessed with the DPPH method. Furthermore, the study identifies the predominant anthocyanin as cyanidin, along with the presence of other anthocyanins such as delphinidin (Dp), malvidin (Mv), petunidin (Pt), pelargonidin (Pg), and peonidin (Pn). Interestingly, the extracted anthocyanins demonstrate pH sensitivity, changing from red to brown as pH increases. These findings highlight the potential of utilizing Cavendish banana inflorescence for anthocyanin extraction, offering sustainable waste valorization methods with promising applications in biomimetics and bioinspiration fields.<\/jats:p>","DOI":"10.3390\/biomimetics9110702","type":"journal-article","created":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T10:44:37Z","timestamp":1732013077000},"page":"702","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Extraction, Isolation, Identification, and Characterization of Anthocyanin from Banana Inflorescence by Liquid Chromatography-Mass Spectroscopy and Its pH Sensitivity"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9190-9567","authenticated-orcid":false,"given":"Nuwanthi","family":"Senevirathna","sequence":"first","affiliation":[{"name":"School of Mechanical, Medical and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane 4000, Australia"},{"name":"Centre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of Technology, Brisbane 4000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morteza","family":"Hassanpour","sequence":"additional","affiliation":[{"name":"School of Mechanical, Medical and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane 4000, Australia"},{"name":"Centre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of Technology, Brisbane 4000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"O\u2019Hara","sequence":"additional","affiliation":[{"name":"School of Mechanical, Medical and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane 4000, Australia"},{"name":"Centre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of Technology, Brisbane 4000, Australia"},{"name":"ARC Centre of Excellence in Synthetic Biology, Queensland University of Technology, Brisbane 4000, Australia"},{"name":"ARC Industrial Transformation Training Centre for Bioplastics and Biocomposites, Queensland University of Technology, Brisbane 4000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9074-0384","authenticated-orcid":false,"given":"Azharul","family":"Karim","sequence":"additional","affiliation":[{"name":"School of Mechanical, Medical and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane 4000, Australia"},{"name":"Centre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of Technology, Brisbane 4000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,15]]},"reference":[{"key":"ref_1","unstructured":"FAO (2023). Banana Market Review, FAO."},{"key":"ref_2","unstructured":"Hort-Innovation (2023). Australian Horticulture Statistics Handbook 2022\/2023, Hort-Innovation."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.tifs.2019.12.023","article-title":"Banana inflorescence: Its bio-prospects as an ingredient for functional foods","volume":"97","author":"Lau","year":"2020","journal-title":"Trends Food Sci. Technol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Liu, Y., Tong, Y., Tong, Q., Xu, W., and Wang, Z. (2023). Effects of sunflower pectin on thermal stability of purple sweet potato anthocyanins at different pH. Int. J. Biol. Macromol., 253.","DOI":"10.1016\/j.ijbiomac.2023.126663"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"136494","DOI":"10.1016\/j.foodchem.2023.136494","article-title":"Effects of whey protein isolate and ferulic acid\/phloridzin\/naringin\/cysteine on the thermal stability of mulberry anthocyanin extract at neutral pH","volume":"425","author":"Cheng","year":"2023","journal-title":"Food Chem."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Avula, B., Katragunta, K., Osman, A.G., Ali, Z., John Adams, S., Chittiboyina, A.G., and Khan, I.A. (2023). Advances in the chemistry, analysis and adulteration of anthocyanin rich-berries and fruits: 2000\u20132022. Molecules, 28.","DOI":"10.3390\/molecules28020560"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1016\/j.nut.2015.04.015","article-title":"Classification of fruits based on anthocyanin types and relevance to their health effects","volume":"31","author":"Fang","year":"2015","journal-title":"Nutrition"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"100051","DOI":"10.1016\/j.fhfh.2021.100051","article-title":"Alginate based encapsulation as a tool for the protection of bioactive compounds from aromatic herbs","volume":"2","year":"2022","journal-title":"Food Hydrocoll. Health"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"10853","DOI":"10.1021\/jf8018529","article-title":"Anthocyanin composition of wild bananas in Thailand","volume":"56","author":"Kitdamrongsont","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"114252","DOI":"10.1016\/j.lwt.2022.114252","article-title":"Effects of environmental factors and fermentation on red raspberry anthocyanins stability","volume":"173","author":"Yuan","year":"2023","journal-title":"LWT"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1016\/j.ijbiomac.2020.01.197","article-title":"Improvement of thermal stability and antioxidant activity of anthocyanins of Echium amoenum petal using maltodextrin\/modified starch combination as wall material","volume":"148","author":"Mehran","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4071","DOI":"10.1007\/s12649-024-02424-4","article-title":"Valorization of black carrot industrial residues for the anthocyanin pigment production","volume":"15","author":"Muro","year":"2024","journal-title":"Waste Biomass Valoris."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.foodchem.2011.08.005","article-title":"Characterization of anthocyanins in Kenyan teas: Extraction and identification","volume":"131","author":"Kerio","year":"2012","journal-title":"Food Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1007\/s43555-024-00021-z","article-title":"Anthocyanin-Based Natural Color Induced Intelligent Food Packaging Sensor: A Review","volume":"2","author":"Kolekar","year":"2024","journal-title":"Curr. Food Sci. Technol. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1016\/j.foodchem.2008.03.010","article-title":"Correlation of two anthocyanin quantification methods: HPLC and spectrophotometric methods","volume":"110","author":"Lee","year":"2008","journal-title":"Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s40011-023-01538-4","article-title":"Preparation and Characterization of Anthocyanin Loaded Lotus Pollen Exine Nanocapsules for its Use in Oral Drug Delivery Applications","volume":"94","author":"Priyadharshini","year":"2024","journal-title":"Proc. Natl. Acad. Sci. India Sect. B Biol. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"7242","DOI":"10.1080\/10408398.2021.1913092","article-title":"Anthocyanin supplement as a dietary strategy in cancer prevention and management: A comprehensive review","volume":"62","author":"Chen","year":"2022","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Liu, J., Zhou, H., Song, L., Yang, Z., Qiu, M., Wang, J., and Shi, S. (2021). Anthocyanins: Promising natural products with diverse pharmacological activities. Molecules, 26.","DOI":"10.3390\/molecules26133807"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"8639","DOI":"10.1080\/10408398.2022.2063250","article-title":"Updated insights into anthocyanin stability behavior from bases to cases: Why and why not anthocyanins lose during food processing","volume":"63","author":"Chen","year":"2023","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Nurtiana, W. (2019). Anthocyanin as natural colorant: A review. Food ScienTech J., 1.","DOI":"10.33512\/fsj.v1i1.6180"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Nawawi, N.I.M., Ijod, G., Abas, F., Ramli, N.S., Mohd Adzahan, N., and Mohamad Azman, E. (2023). Influence of Different Drying Methods on Anthocyanins Composition and Antioxidant Activities of Mangosteen (Garcinia mangostana L.) Pericarps and LC-MS Analysis of the Active Extract. Foods, 12.","DOI":"10.3390\/foods12122351"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Wu, Y., Gao, Y., and Li, C. (2023). Preparation and characterization of smart indicator films based on gellan gum\/modified black rice anthocyanin\/curcumin for improving the stability of natural anthocyanins. Int. J. Biol. Macromol., 253.","DOI":"10.1016\/j.ijbiomac.2023.127436"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Le, X.T., Huynh, M.T., Pham, T.N., Than, V.T., Toan, T.Q., Bach, L.G., and Trung, N.Q. (2019). Optimization of Total Anthocyanin Content, Stability and Antioxidant Evaluation of the Anthocyanin Extract from Vietnamese Carissa carandas L. Fruits. Processes, 7.","DOI":"10.3390\/pr7070468"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1361779","DOI":"10.1080\/16546628.2017.1361779","article-title":"Anthocyanidins and anthocyanins: Colored pigments as food, pharmaceutical ingredients, and the potential health benefits","volume":"61","author":"Khoo","year":"2017","journal-title":"Food Nutr. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1080\/10408347.2012.680341","article-title":"Analysis and Antioxidant Capacity of Anthocyanin Pigments. Part III: An Introduction to Sample Preparation and Extraction","volume":"42","author":"Navas","year":"2012","journal-title":"Crit. Rev. Anal. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"137155","DOI":"10.1016\/j.foodchem.2023.137155","article-title":"In situ Raman characterization of the stability of blueberry anthocyanins in aqueous solutions under perturbations in temperature, UV, pH","volume":"431","author":"Duan","year":"2024","journal-title":"Food Chem."},{"key":"ref_27","first-page":"20229","article-title":"Biodegradable pH sensor in packaging material using anthocyanin from banana bracts","volume":"14","author":"George","year":"2023","journal-title":"Biomass Convers. Biorefinery"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.powtec.2017.01.078","article-title":"Combining various wall materials for encapsulation of blueberry anthocyanin extracts: Optimization by artificial neural network and genetic algorithm and a comprehensive analysis of anthocyanin powder properties","volume":"311","author":"Tao","year":"2017","journal-title":"Powder Technol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"117488","DOI":"10.1016\/j.carbpol.2020.117488","article-title":"Carbohydrate-based films containing pH-sensitive red barberry anthocyanins: Application as biodegradable smart food packaging materials","volume":"255","author":"Sani","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3006","DOI":"10.3390\/ijms12053006","article-title":"Optimization of ultrasound-assisted extraction of anthocyanins from mulberry, using response surface methodology","volume":"12","author":"Zou","year":"2011","journal-title":"Int. J. Mol. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"125535","DOI":"10.1016\/j.foodchem.2019.125535","article-title":"Microencapsulation by spray-drying of polyphenols extracted from red chicory and red cabbage: Effects on stability and color properties","volume":"307","author":"Zanoni","year":"2020","journal-title":"Food Chem."},{"key":"ref_32","first-page":"498","article-title":"Evaluation of antioxidant activity of extracts of banana inflorescences (Musa cavendishii)","volume":"13","author":"Schmidt","year":"2015","journal-title":"CYTA J. Food"},{"key":"ref_33","first-page":"438","article-title":"Mechanisms of ginger bioactive compounds extract using Soxhlet and accelerated water extraction","volume":"8","author":"Azian","year":"2014","journal-title":"Int. J. Chem. Mater. Sci. Eng."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2354","DOI":"10.1007\/s13197-020-04273-0","article-title":"Ultrasound-assisted extracted dietary fibre from culinary banana bract as matrices for anthocyanin: Its preparation, characterization and storage stability","volume":"57","author":"Begum","year":"2020","journal-title":"J. Food Sci. Technol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/S0076-6879(99)99017-1","article-title":"[14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent","volume":"299","author":"Singleton","year":"1999","journal-title":"Methods Enzymol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3452","DOI":"10.1021\/jf010172v","article-title":"Free radical scavenging properties of conjugated linoleic acids","volume":"49","author":"Yu","year":"2001","journal-title":"J. Agric. Food Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1007\/s11483-022-09727-w","article-title":"Effects of anthocyanin-rich extract on the physical, antioxidant, and pH-sensitive properties of biodegradable film","volume":"17","author":"Yan","year":"2022","journal-title":"Food Biophys."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"110473","DOI":"10.1016\/j.lwt.2020.110473","article-title":"Microencapsulation of red cabbage anthocyanin-rich extract by drum drying technique","volume":"137","author":"Sakulnarmrat","year":"2021","journal-title":"LWT"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"100282","DOI":"10.1016\/j.afres.2023.100282","article-title":"FTIR spectra, antioxidant capacity and degradation kinetics of maize anthocyanin extract under variable process conditions","volume":"3","author":"Bhushan","year":"2023","journal-title":"Appl. Food Res."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Mattioli, R., Francioso, A., Mosca, L., and Silva, P. (2020). Anthocyanins: A comprehensive review of their chemical properties and health effects on cardiovascular and neurodegenerative diseases. Molecules, 25.","DOI":"10.3390\/molecules25173809"},{"key":"ref_41","first-page":"264","article-title":"Effect of sun, oven and freeze-drying on anthocyanins, phenolic compounds and antioxidant activity of black grape (Ek\u015fikara) (Vitis vinifera L.)","volume":"38","author":"Akbulut","year":"2017","journal-title":"S. Afr. J. Enol. Vitic."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"5601","DOI":"10.1111\/ijfs.15072","article-title":"A potential of banana flower and pseudo-stem as novel ingredients rich in phenolic compounds","volume":"56","author":"Robaina","year":"2021","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"8178294","DOI":"10.1155\/2019\/8178294","article-title":"Evaluation of the Use of Different Solvents for Phytochemical Constituents, Antioxidants, and In Vitro Anti-Inflammatory Activities of Severinia buxifolia","volume":"2019","author":"Truong","year":"2019","journal-title":"J. Food Qual."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4477","DOI":"10.1021\/jf049595y","article-title":"Distribution and contents of phenolic compounds in eighteen Scandinavian berry species","volume":"52","author":"Mattila","year":"2004","journal-title":"J. Agric. Food Chem."},{"key":"ref_45","first-page":"243","article-title":"Comparative study on conventional, accelerated solvent extraction and ultrasonic-assisted extraction of total phenolic and anthocyanin contents and antioxidant activities from Riceberry bran","volume":"56","author":"Sirichokworrakit","year":"2022","journal-title":"Agric. Nat. Resour."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3135","DOI":"10.1080\/10942912.2016.1277739","article-title":"Stability of spray-dried microencapsulated anthocyanins extracted from culinary banana bract","volume":"20","author":"Begum","year":"2017","journal-title":"Int. J. Food Prop."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Nistor, M., Pop, R., Daescu, A., Pintea, A., Socaciu, C., and Rugina, D. (2022). Anthocyanins as key phytochemicals acting for the prevention of metabolic diseases: An overview. Molecules, 27.","DOI":"10.3390\/molecules27134254"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Kumkum, R., Aston-Mourney, K., McNeill, B.A., Hern\u00e1ndez, D., and Rivera, L.R. (2024). Bioavailability of Anthocyanins: Whole Foods versus Extracts. Nutrients, 16.","DOI":"10.3390\/nu16101403"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Kamiloglu, S., and Akgun, B. (2023). Petunidin: Advances on Resources, Biosynthesis Pathway, Bioavailability, Bioactivity, and Pharmacology. Handbook of Dietary Flavonoids, Springer.","DOI":"10.1007\/978-3-030-94753-8_58-1"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1207\/s15327914nc5302_12","article-title":"Cyanidin 3-Glucoside and Peonidin 3-Glucoside Inhibit Tumor Cell Growth and Induce Apoptosis In Vitro and Suppress Tumor Growth In Vivo","volume":"53","author":"Chen","year":"2005","journal-title":"Nutr. Cancer"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.tifs.2020.08.014","article-title":"pH-sensitive (halochromic) smart packaging films based on natural food colorants for the monitoring of food quality","volume":"105","author":"Mohammadian","year":"2020","journal-title":"Trends Food Sci. Technol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.foodhyd.2018.12.015","article-title":"Development of antioxidant and intelligent pH-sensing packaging films by incorporating purple-fleshed sweet potato extract into chitosan matrix","volume":"90","author":"Yong","year":"2019","journal-title":"Food Hydrocoll."}],"container-title":["Biomimetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2313-7673\/9\/11\/702\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:33:26Z","timestamp":1760114006000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2313-7673\/9\/11\/702"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,15]]},"references-count":52,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2024,11]]}},"alternative-id":["biomimetics9110702"],"URL":"https:\/\/doi.org\/10.3390\/biomimetics9110702","relation":{},"ISSN":["2313-7673"],"issn-type":[{"value":"2313-7673","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,15]]}}}