{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T11:54:32Z","timestamp":1780055672526,"version":"3.54.0"},"reference-count":38,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,5,16]],"date-time":"2021-05-16T00:00:00Z","timestamp":1621123200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Council for Scientific and Technological Development (CNPq).","award":["440605\/2016-4"],"award-info":[{"award-number":["440605\/2016-4"]}]},{"name":"Coordination for the Improvement of Higher Education Personnel (CAPES) Brazilian foundations","award":["0001"],"award-info":[{"award-number":["0001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Zika virus (ZIKV) is a mosquito-borne infection, predominant in tropical and subtropical regions causing international concern due to the ZIKV disease having been associated with congenital disabilities, especially microcephaly and other congenital abnormalities in the fetus and newborns. Development of strategies that minimize the devastating impact by monitoring and preventing ZIKV transmission through sexual intercourse, especially in pregnant women, since no vaccine is yet available for the prevention or treatment, is critically important. ZIKV infection is generally asymptomatic and cross-reactivity with dengue virus (DENV) is a global concern. An innovative screen-printed electrode (SPE) was developed for amperometric detection of the non-structural protein (NS2B) of ZIKV by exploring the intrinsic redox catalytic activity of Prussian blue (PB), incorporated into a carbon nanotube\u2013polypyrrole composite. Thus, this immunosensor has the advantage of electrochemical detection without adding any redox-probe solution (probe-less detection), allowing a point-of-care diagnosis. It was responsive to serum samples of only ZIKV positive patients and non-responsive to negative ZIKV patients, even if the sample was DENV positive, indicating a possible differential diagnosis between them by NS2B. All samples used here were confirmed by CDC protocols, and immunosensor responses were also checked in the supernatant of C6\/36 and in Vero cell cultures infected with ZIKV.<\/jats:p>","DOI":"10.3390\/bios11050157","type":"journal-article","created":{"date-parts":[[2021,5,16]],"date-time":"2021-05-16T23:17:16Z","timestamp":1621207036000},"page":"157","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["A Label and Probe-Free Zika Virus Immunosensor Prussian Blue@carbon Nanotube-Based for Amperometric Detection of the NS2B Protein"],"prefix":"10.3390","volume":"11","author":[{"given":"B\u00e1rbara V. M.","family":"Silva","sequence":"first","affiliation":[{"name":"Biomedical Engineering Laboratory, Department of Biomedical Engineering, Federal University of Pernambuco, Recife 50670-901, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marli T.","family":"Cordeiro","sequence":"additional","affiliation":[{"name":"Department of Virology and Experimental Therapy, Oswaldo Cruz Foundation\u2014FIOCRUZ, Aggeu Magalh\u00e3es Institute, Recife 50670-420, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5143-3696","authenticated-orcid":false,"given":"Marco A. B.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Electronics and Systems, Federal University of Pernambuco, Recife 50670-901, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3826-9358","authenticated-orcid":false,"given":"Ernesto T. A.","family":"Marques","sequence":"additional","affiliation":[{"name":"Center for Vaccine Research, Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA 15213, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8314-4218","authenticated-orcid":false,"given":"Rosa F.","family":"Dutra","sequence":"additional","affiliation":[{"name":"Biomedical Engineering Laboratory, Department of Biomedical Engineering, Federal University of Pernambuco, Recife 50670-901, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"i657","DOI":"10.1136\/bmj.i657","article-title":"Zika virus is a global public health emergency, declares WHO","volume":"352","author":"Gulland","year":"2016","journal-title":"BMJ"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1016\/S0140-6736(17)31368-5","article-title":"Infection-related microcephaly after the 2015 and 2016 Zika virus outbreaks in Brazil: A surveillance-based analysis","volume":"390","author":"Carmo","year":"2017","journal-title":"Lancet"},{"key":"ref_3","first-page":"20","article-title":"Zika virus infection, basic and clinical aspects: A review article","volume":"48","author":"Noorbakhsh","year":"2019","journal-title":"Iran. 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