{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T18:27:27Z","timestamp":1787682447559,"version":"build-2784847793"},"reference-count":62,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,6,10]],"date-time":"2021-06-10T00:00:00Z","timestamp":1623283200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NAS of Ukraine","award":["0118U006260"],"award-info":[{"award-number":["0118U006260"]}]},{"DOI":"10.13039\/501100007684","name":"Ministry of Education and Science of Ukraine","doi-asserted-by":"publisher","award":["0120U103398"],"award-info":[{"award-number":["0120U103398"]}],"id":[{"id":"10.13039\/501100007684","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Research Foundation of Ukraine","award":["48\/02.2020"],"award-info":[{"award-number":["48\/02.2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Prussian blue analogs (PBAs) are well-known artificial enzymes with peroxidase (PO)-like activity. PBAs have a high potential for applications in scientific investigations, industry, ecology and medicine. Being stable and both catalytically and electrochemically active, PBAs are promising in the construction of biosensors and biofuel cells. The \u201cgreen\u201d synthesis of PO-like PBAs using oxido-reductase flavocytochrome b2 is described in this study. When immobilized on graphite electrodes (GEs), the obtained green-synthesized PBAs or hexacyanoferrates (gHCFs) of transition and noble metals produced amperometric signals in response to H2O2. HCFs of copper, iron, palladium and other metals were synthesized and characterized by structure, size, catalytic properties and electro-mediator activities. The gCuHCF, as the most effective PO mimetic with a flower-like micro\/nano superstructure, was used as an H2O2-sensitive platform for the development of a glucose oxidase (GO)-based biosensor. The GO\/gCuHCF\/GE biosensor exhibited high sensitivity (710 A M\u22121m\u22122), a broad linear range and good selectivity when tested on real samples of fruit juices. We propose that the gCuHCF and other gHCFs synthesized via enzymes may be used as artificial POs in amperometric oxidase-based (bio)sensors.<\/jats:p>","DOI":"10.3390\/bios11060193","type":"journal-article","created":{"date-parts":[[2021,6,10]],"date-time":"2021-06-10T13:31:39Z","timestamp":1623331899000},"page":"193","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["\u201cGreen\u201d Prussian Blue Analogues as Peroxidase Mimetics for Amperometric Sensing and Biosensing"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4015-8083","authenticated-orcid":false,"given":"Galina Z.","family":"Gayda","sequence":"first","affiliation":[{"name":"Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olha M.","family":"Demkiv","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine"},{"name":"Faculty of Veterinary Hygiene, Ecology and Law, Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, 79000 Lviv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4525-4189","authenticated-orcid":false,"given":"Yanna","family":"Gurianov","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Ariel University, Kyriat-ha-Mada, Ariel 4070000, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman Ya.","family":"Serkiz","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Halyna M.","family":"Klepach","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Natural Sciences, Drohobych Ivan Franko State Pedagogical University, 82100 Drohobych, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mykhailo V.","family":"Gonchar","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine"},{"name":"Faculty of Biology and Natural Sciences, Drohobych Ivan Franko State Pedagogical University, 82100 Drohobych, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8493-713X","authenticated-orcid":false,"given":"Marina","family":"Nisnevitch","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Ariel University, Kyriat-ha-Mada, Ariel 4070000, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"100452","DOI":"10.1016\/j.cogsc.2021.100452","article-title":"Artificial enzymes with multiple active sites","volume":"29","author":"Palomo","year":"2021","journal-title":"Curr. 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