{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T04:59:55Z","timestamp":1791608395951,"version":"4.3.4"},"reference-count":40,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,7,3]],"date-time":"2021-07-03T00:00:00Z","timestamp":1625270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004737","name":"National Health Research Institutes","doi-asserted-by":"publisher","award":["EM-110-SP-01"],"award-info":[{"award-number":["EM-110-SP-01"]}],"id":[{"id":"10.13039\/501100004737","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>The issue of micro-plastics is becoming more and more important due to their ubiquity and the harm they cause to the human body. Therefore, evaluating the biological\u2013physical interaction of micro-plastics with health cells has become the focus of many research efforts. This study focuses on the movement mode and low concentration detection development for micro-plastics in surface plasmon resonance (SPR). Firstly, 20-micrometer micro-plastics were prepared by grinding and filtering, and the movement mode was explored; then, the characteristics were investigated by SPR. Chromatographic analysis showed that the surface charge of micro-plastics dominated the elution time, and estrogen receptors (ERs) played a supporting role. A difference of micro-plastics in SPR sensorgram was observed, inferring the micro-plastics\u2019 movement in rolling mode on the ERs. Characteristics analysis indicated that the low particle number of micro-plastics on SPR showed a linear relationship with the response unit (RU). When ERs were immobilized on the biosensor, the force of the binding of micro-plastics to ERs under an ultra-low background was equivalent to the dissociation rate constant shown as follows: PS (0.05 nM) &gt; PVC (0.09 nM) &gt; PE (0.14 nM). The ELISA-like magnetic beads experiment verified the specificity between ERs and micro-plastics. Therefore, by using the SPR technique, a biological-derived over-occupation of PS was found via higher binding force with ERs and longer retention time. In the future, there will be considerable potential for micro-plastics issues, such as identification in natural samples, biomarking, real-time detection in specific environments\/regions and human health subject.<\/jats:p>","DOI":"10.3390\/bios11070219","type":"journal-article","created":{"date-parts":[[2021,7,4]],"date-time":"2021-07-04T22:34:36Z","timestamp":1625438076000},"page":"219","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Measurement of Low Concentration of Micro-Plastics by Detection of Bioaffinity-Induced Particle Retention Using Surface Plasmon Resonance Biosensors"],"prefix":"10.3390","volume":"11","author":[{"given":"Chen-Ji","family":"Huang","sequence":"first","affiliation":[{"name":"Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Maoli 350, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gudivada Vijaya","family":"Narasimha","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Maoli 350, Taiwan"},{"name":"Department of Life Sciences, National Central University, Taoyuan 320, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Cheng","family":"Chen","sequence":"additional","affiliation":[{"name":"National Institute of Environmental Health Sciences, National Health Research Institutes, Maoli 350, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3433-5971","authenticated-orcid":false,"given":"Jen-Kun","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Maoli 350, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo-Chung","family":"Dong","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Maoli 350, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"eaax1157","DOI":"10.1126\/sciadv.aax1157","article-title":"White and wonderful? 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