{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T22:18:41Z","timestamp":1781302721162,"version":"3.54.1"},"reference-count":60,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T00:00:00Z","timestamp":1635206400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002632","name":"Hallym University","doi-asserted-by":"publisher","award":["HRF-202012-009"],"award-info":[{"award-number":["HRF-202012-009"]}],"id":[{"id":"10.13039\/501100002632","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>The measurement of cysteine in human urine and live cells is crucial for evaluating biological metabolism, monitoring and maintaining the immune system, preventing tissue\/DNA damage caused by free radicals, preventing autoimmune diseases, and diagnosing disorders such as cystinuria and cancer. A method that uses a fluorescence turn-on probe and a portable fluorescence spectrometer device are crucial for highly sensitive, simple, rapid, and inexpensive cysteine detection. Herein, we present the synthesis and application of a benzimidazole-based fluorescent probe (ABIA) along with the design and development of a portable fluorescence spectrometer device (CysDDev) for detecting cysteine in simulated human urine. ABIA showed excellent selectivity and sensitivity in detecting cysteine over homocysteine, glutathione, and other amino acids with the response time of 1 min and demonstrated a detection limit of 16.3 nM using the developed CysDDev. Further, ABIA also demonstrated its utility in detecting intracellular cysteine, making it an excellent probe for bio-imaging assay.<\/jats:p>","DOI":"10.3390\/bios11110420","type":"journal-article","created":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T23:49:52Z","timestamp":1635292192000},"page":"420","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine"],"prefix":"10.3390","volume":"11","author":[{"given":"Gyu Seong","family":"Yeom","sequence":"first","affiliation":[{"name":"Institute of Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 24252, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In-ho","family":"Song","sequence":"additional","affiliation":[{"name":"Institute of Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 24252, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shrikant Dashrath","family":"Warkad","sequence":"additional","affiliation":[{"name":"Biometrix Technology, Inc., 2-2 Bio Venture Plaza 56, Chuncheon 24232, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3173-0896","authenticated-orcid":false,"given":"Pramod B.","family":"Shinde","sequence":"additional","affiliation":[{"name":"Natural Products & Green Chemistry Division, CSIR\u2014Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific and Industrial Research (CSIR), Bhavnagar 364002, Gujarat, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taewoon","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Software, Hallym University, Chuncheon 24252, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong-min","family":"Park","sequence":"additional","affiliation":[{"name":"Institute of Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 24252, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5137-0584","authenticated-orcid":false,"given":"Satish Balasaheb","family":"Nimse","sequence":"additional","affiliation":[{"name":"Institute of Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 24252, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"947","DOI":"10.3389\/fonc.2020.00947","article-title":"Cysteine as a Carbon Source, a Hot Spot in Cancer Cells Survival","volume":"10","author":"Serpa","year":"2020","journal-title":"Front. 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