{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T16:02:26Z","timestamp":1774540946260,"version":"3.50.1"},"reference-count":44,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T00:00:00Z","timestamp":1651017600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>The binding of drugs to DNA plays a critical role in new drug discovery and is important for designing better drugs. In this study, the interaction and binding mode of calf-thymus double-stranded deoxyribonucleic acid (ct-dsDNA) with cinacalcet (CIN) from the calcimimetic drug that mimics the action of calcium on tissues group were investigated. The interaction of CIN with ct-dsDNA was observed by the differential pulse voltammetry (DPV) technique by following the decrease in electrochemical oxidation signals to deoxyguanosine and adenosine. A competitive study was performed on an indicator, methylene blue, to investigate the interaction of the drug with ct-dsDNA by fluorescence spectroscopy. Interaction studies have shown that the binding mode for the interaction of CIN with ct-dsDNA could be groove-binding. According to the results obtained, the binding constant values were found to be 6.30 \u00d7 104 M\u22121 and 3.16 \u00d7 105 M\u22121, respectively, at 25 \u00b0C as obtained from the cyclic voltammetry (CV) and spectroscopic techniques. Possible molecular interactions of CIN with dsDNA were explored via molecular docking experiments. The docked structure indicated that CIN could fit well into the minor groove of the DNA through H-bonding and \u03c0-\u03c0 stacking contact with CIN.<\/jats:p>","DOI":"10.3390\/bios12050278","type":"journal-article","created":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T13:40:57Z","timestamp":1651066857000},"page":"278","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Evaluation of the Interaction of Cinacalcet with Calf Thymus dsDNA: Use of Electrochemical, Spectrofluorimetric, and Molecular Docking Methods"],"prefix":"10.3390","volume":"12","author":[{"given":"Cem","family":"Erkmen","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Ankara 06560, Turkey"},{"name":"The Graduate School of Health Sciences, Ankara University, Ankara 06110, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Didem Nur","family":"Unal","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Ankara 06560, Turkey"},{"name":"The Graduate School of Health Sciences, Ankara University, Ankara 06110, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7079-7604","authenticated-orcid":false,"given":"Sevinc","family":"Kurbanoglu","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Ankara 06560, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3420-607X","authenticated-orcid":false,"given":"Gokcen","family":"Eren","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gazi University, Etiler, Ankara 06330, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bengi","family":"Uslu","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Ankara 06560, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"913","DOI":"10.2215\/CJN.06040710","article-title":"Secondary Hyperparathyroidism: Pathogenesis, Disease Progression, and Therapeutic Options","volume":"6","author":"Cunningham","year":"2011","journal-title":"Clin. J. Am. Soc. Nephrol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2830","DOI":"10.1016\/j.ekir.2021.08.020","article-title":"Efficacy of Evocalcet in Previously Cinacalcet-Treated Secondary Hyperparathyroidism Patients","volume":"6","author":"Koiwa","year":"2021","journal-title":"Kidney Int. Rep."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"271","DOI":"10.2165\/00003495-200565020-00007","article-title":"Cinacalcet Hydrochloride","volume":"65","author":"Balfour","year":"2005","journal-title":"Drugs"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1210\/jcem.86.2.7207","article-title":"Clinical Review 122: Parathyroid Carcinoma","volume":"86","author":"Shane","year":"2001","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1124\/jpet.103.057273","article-title":"Pharmacodynamics of the Type II Calcimimetic Compound Cinacalcet HCl","volume":"308","author":"Nemeth","year":"2004","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.1007\/s11255-014-0723-0","article-title":"Cinacalcet May Improve Oxidative DNA Damage in Maintenance Hemodialysis Patients: An Observational Study","volume":"46","author":"Ari","year":"2014","journal-title":"Int. Urol. Nephrol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.2215\/CJN.11141016","article-title":"Calcium-Sensing Receptor Genotype and Response to Cinacalcet in Patients Undergoing Hemodialysis","volume":"12","author":"Moe","year":"2017","journal-title":"Clin. J. Am. Soc. Nephrol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3427","DOI":"10.1021\/cr200303p","article-title":"Electrochemistry of Nucleic Acids","volume":"112","year":"2012","journal-title":"Chem. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1351\/PAC-REP-09-08-16","article-title":"Electrochemical Nucleic Acid-Based Biosensors: Concepts, Terms, and Methodology (IUPAC Technical Report)","volume":"82","author":"Labuda","year":"2010","journal-title":"Pure Appl. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"116098","DOI":"10.1016\/j.trac.2020.116098","article-title":"DNA-Based Label-Free Electrochemical Biosensors: From Principles to Applications","volume":"133","author":"Hai","year":"2020","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.jelechem.2016.05.022","article-title":"Advances in Electrochemical DNA Biosensors and Their Interaction Mechanism with Pharmaceuticals","volume":"775","author":"Kurbanoglu","year":"2016","journal-title":"J. Electroanal. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Wu, Q., Zhang, Y., Yang, Q., Yuan, N., and Zhang, W. (2019). Review of Electrochemical DNA Biosensors for Detecting Food Borne Pathogens. Sensors, 19.","DOI":"10.3390\/s19224916"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.trac.2016.01.019","article-title":"Applications of a DNA-Electrochemical Biosensor","volume":"79","author":"Diculescu","year":"2016","journal-title":"TrAC - Trends Anal. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Bozal-Palabiyik, B., Kurbanoglu, S., Erkmen, C., and Uslu, B. (2021). Future Prospects and Concluding Remarks for Electroanalytical Applications of Quantum Dots, Elsevier Inc.","DOI":"10.1016\/B978-0-12-821670-5.00008-7"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.bioelechem.2018.05.004","article-title":"Interaction of DNA and Mononucleotides with Theophylline Investigated Using Electrochemical Biosensors and Biosensing","volume":"123","author":"Labuda","year":"2018","journal-title":"Bioelectrochemistry"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"127502","DOI":"10.1149\/1945-7111\/aba8b7","article-title":"A Label-Free DNA Biosensor for Determination of Topotecan as an Anticancer Drug: Electrochemical, Spectroscopic and Docking Studies","volume":"167","author":"Javar","year":"2020","journal-title":"J. Electrochem. Soc."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1039\/C9AN01976F","article-title":"An Electrochemical Aptamer-Based Sensor Prepared by Utilizing the Strong Interaction between a DNA Aptamer and Diamond","volume":"145","author":"Asai","year":"2020","journal-title":"Analyst"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"107579","DOI":"10.1016\/j.bioelechem.2020.107579","article-title":"An Electrochemical Study of 9-Chloroacridine Redox Behavior and Its Interaction with Double-Stranded DNA","volume":"135","author":"Rupar","year":"2020","journal-title":"Bioelectrochemistry"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Ozkan-Ariksoysal, D. (2021). Electrochemical DNA Biosensors Based on Quantum Dots, Elsevier Inc.","DOI":"10.1016\/B978-0-12-821670-5.00004-X"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5124","DOI":"10.1021\/acsomega.0c02612","article-title":"Mechanism of Interactions of DsDNA Binding with Apigenin and Its Sulfamate Derivatives Using Multispectroscopic, Voltammetric, and Molecular Docking Studies","volume":"6","author":"Waihenya","year":"2021","journal-title":"ACS Omega"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"115098","DOI":"10.1016\/j.jelechem.2021.115098","article-title":"Probing the Interaction of Ciprofloxacin with DsDNA: Electrochemical, Spectro-Electrochemical and AFM Investigation","volume":"885","author":"Ipte","year":"2021","journal-title":"J. Electroanal. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"112994","DOI":"10.1016\/j.jpba.2019.112994","article-title":"Elucidation of Binding Interactions and Mechanism of Fludarabine with DsDNA via Multispectroscopic and Molecular Docking Studies","volume":"179","author":"Agar","year":"2020","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_23","first-page":"5436","article-title":"Chemometrics and Monte-Carlo Simulation Assisted RP-LC Method for Estimation of Cinacalcet Hydro-Chloride in Pharmaceutical Products","volume":"14","author":"Panda","year":"2021","journal-title":"Int. J. Pharm. Sci. Nanotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.jchromb.2016.05.047","article-title":"Separation and Quantitative Determination of Cinacalcet Metabolites in Urine Sample Using RP-HPLC after Derivation with a Fluorescent Labeling Reagent","volume":"1027","author":"Jaymand","year":"2016","journal-title":"J. Chromatogr. B"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"5076","DOI":"10.1039\/c4ay00077c","article-title":"Liquid Chromatographic Separation, Determination and ESI-MS\/MS, FT-IR and NMR Characterization of the Forced Degradation Products of Cinacalcet","volume":"6","author":"Rao","year":"2014","journal-title":"Anal. Methods"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2179","DOI":"10.1073\/pnas.78.4.2179","article-title":"Structure of a B-DNA Dodecamer: Conformation and Dynamics","volume":"78","author":"Drew","year":"1981","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1021\/jm030644s","article-title":"Glide: A New Approach for Rapid, Accurate Docking and Scoring. 2. Enrichment Factors in Database Screening","volume":"47","author":"Halgren","year":"2004","journal-title":"J. Med. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"6177","DOI":"10.1021\/jm051256o","article-title":"Extra Precision Glide:\u2009 Docking and Scoring Incorporating a Model of Hydrophobic Enclosure for Protein\u2212Ligand Complexes","volume":"49","author":"Friesner","year":"2006","journal-title":"J. Med. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.1002\/elan.201900162","article-title":"Antidiabetic Drug Metformin Oxidation and in Situ Interaction with DsDNA Using a DsDNA-electrochemical Biosensor","volume":"31","author":"Machini","year":"2019","journal-title":"Electroanalysis"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1016\/j.msec.2015.12.020","article-title":"Pharmacogenomic Study Using Bio- and Nanobioelectrochemistry: Drug-DNA Interaction","volume":"61","author":"Hasanzadeh","year":"2016","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.talanta.2007.10.012","article-title":"Nanomaterial-Based Electrochemical DNA Sensing Strategies","volume":"74","author":"Erdem","year":"2007","journal-title":"Talanta"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1002\/1521-4109(200208)14:14<965::AID-ELAN965>3.0.CO;2-U","article-title":"Electrochemical DNA Biosensors Based on DNA-Drug Interactions","volume":"14","author":"Erdem","year":"2002","journal-title":"Electroanalysis"},{"key":"ref_33","first-page":"1451","article-title":"A Reduced Graphene Oxide-based Electrochemical DNA Biosensor for the Detection of Interaction between Cisplatin and DNA Based on Guanine and Adenine","volume":"29","author":"Vandeput","year":"2017","journal-title":"Wiley Online Libr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/0302-4598(82)80005-6","article-title":"A Comparison of the Peroxidase-Catalyzed and Electrochemical Oxidation of Uric Acid","volume":"9","author":"Goyal","year":"1982","journal-title":"Bioelectrochemistry Bioenerg."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"027521","DOI":"10.1149\/1945-7111\/ac55cb","article-title":"Phosphodiesterase-3 Enzyme Inhibitor Drug Milrinone Interaction with DNA and HSA: Electrochemical, Spectroscopic and Molecular Docking","volume":"169","author":"Unal","year":"2022","journal-title":"J. Electrochem. Soc."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/0003-2670(96)00248-6","article-title":"Accumulation and Trace Measurements of Phenothiazine Drugs at DNA-Modified Electrodes","volume":"332","author":"Wang","year":"1996","journal-title":"Anal. Chim. Acta"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Ponkarpagam, S., Mahalakshmi, G., Vennila, K.N., and Elango, K.P. (2021). Concentration-Dependent Mode of Binding of Drug Oxatomide with DNA: Multi-Spectroscopic, Voltammetric and Metadynamics Simulation Analysis Multi-Spectroscopic, Voltammetric and Metadynamics Simulation Analysis. J. Biomol. Struct. Dyn., 1\u201311.","DOI":"10.1080\/07391102.2021.1911860"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1080\/15257770.2016.1218019","article-title":"Experimental and Computational Studies on the Effects of Valganciclovir as an Antiviral Drug on Calf Thymus DNA","volume":"36","author":"Shahabadi","year":"2017","journal-title":"Nucleosides Nucleotides Nucleic Acids"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.ijbiomac.2018.03.040","article-title":"Direct Evidences for the Groove Binding of the Clomifene to Double Stranded DNA","volume":"114","author":"Moradi","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.jmgm.2012.05.006","article-title":"Early Stage Intercalation of Doxorubicin to DNA Fragments Observed in Molecular Dynamics Binding Simulations","volume":"38","author":"Lei","year":"2012","journal-title":"J. Mol. Graph. Model."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jphotobiol.2013.03.013","article-title":"Drug-DNA Interactions and Their Study by UV-Visible, Fluorescence Spectroscopies and Cyclic Voltametry","volume":"124","author":"Sirajuddin","year":"2013","journal-title":"J. Photochem. Photobiol. B Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1007\/s10904-010-9370-9","article-title":"Synthesis, Structure and Voltammetric Studies of Copper(II) Polymer with DNA Interaction: The First 1-D Coordination Polymer Alternately Bridged by Oxalate and Oxamidate Ligands","volume":"20","author":"Li","year":"2010","journal-title":"J. Inorg. Organomet. Polym. Mater."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0003-9861(02)00202-3","article-title":"Thermodynamics of Drug\u2013DNA Interactions","volume":"403","author":"Haq","year":"2002","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1002\/bip.10001","article-title":"Spectroscopic Studies of Interaction of Chlorobenzylidine with DNA","volume":"62","author":"Zhong","year":"2001","journal-title":"Biopolymers"}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/12\/5\/278\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:02:00Z","timestamp":1760137320000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/12\/5\/278"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,27]]},"references-count":44,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,5]]}},"alternative-id":["bios12050278"],"URL":"https:\/\/doi.org\/10.3390\/bios12050278","relation":{},"ISSN":["2079-6374"],"issn-type":[{"value":"2079-6374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,27]]}}}