{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:11:39Z","timestamp":1788480699511,"version":"build-2803163510"},"reference-count":25,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,5,11]],"date-time":"2022-05-11T00:00:00Z","timestamp":1652227200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Innovation Team Project of Foshan, P. R. China","award":["No.1920001000047"],"award-info":[{"award-number":["No.1920001000047"]}]},{"name":"Science and Technology Innovation Team Project of Foshan, P. R. China","award":["JSSCTD202145"],"award-info":[{"award-number":["JSSCTD202145"]}]},{"name":"Science and Technology Innovation Team Project of Foshan, P. R. China","award":["No.BZ2018040"],"award-info":[{"award-number":["No.BZ2018040"]}]},{"name":"Innovation and Entrepreneurship Team of Jiangsu Province","award":["No.1920001000047"],"award-info":[{"award-number":["No.1920001000047"]}]},{"name":"Innovation and Entrepreneurship Team of Jiangsu Province","award":["JSSCTD202145"],"award-info":[{"award-number":["JSSCTD202145"]}]},{"name":"Innovation and Entrepreneurship Team of Jiangsu Province","award":["No.BZ2018040"],"award-info":[{"award-number":["No.BZ2018040"]}]},{"name":"Policy Guidance project (International Science and Technology Cooperation) of Jiangsu Province of China","award":["No.1920001000047"],"award-info":[{"award-number":["No.1920001000047"]}]},{"name":"Policy Guidance project (International Science and Technology Cooperation) of Jiangsu Province of China","award":["JSSCTD202145"],"award-info":[{"award-number":["JSSCTD202145"]}]},{"name":"Policy Guidance project (International Science and Technology Cooperation) of Jiangsu Province of China","award":["No.BZ2018040"],"award-info":[{"award-number":["No.BZ2018040"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>In this study, an \u201call-in-one\u201d digital microfluidics (DMF) system was developed for automatic and rapid molecular diagnosis and integrated with magnetic bead-based nucleic acid extraction, loop-mediated isothermal amplification (LAMP), and real-time optical signal monitoring. First, we performed on- and off-chip comparison experiments for the magnetic bead nucleic acid extraction module and LAMP amplification function. The extraction efficiency for the on-chip test was comparable to that of conventional off-chip methods. The processing time for the automatic on-chip workflow was only 23 min, which was less than that of the conventional methods of 28 min 45 s. Meanwhile, the number of samples used in on-chip experiments was significantly smaller than that used in off-chip experiments; only 5 \u00b5L of E. coli samples was required for nucleic acid extraction, and 1 \u00b5L of the nucleic acid template was needed for the amplification reaction. In addition, we selected SARS-CoV-2 nucleic acid reference materials for the nucleic acid detection experiment, demonstrating a limit of detection of 10 copies\/\u00b5L. The proposed \u201call-in-one\u201d DMF system provides an on-site \u201csample to answer\u201d time of approximately 60 min, which can be a powerful tool for point-of-care molecular diagnostics.<\/jats:p>","DOI":"10.3390\/bios12050324","type":"journal-article","created":{"date-parts":[[2022,5,11]],"date-time":"2022-05-11T10:19:46Z","timestamp":1652264386000},"page":"324","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["All-in-One Digital Microfluidics System for Molecular Diagnosis with Loop-Mediated Isothermal Amplification"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0686-5182","authenticated-orcid":false,"given":"Siyi","family":"Hu","sequence":"first","affiliation":[{"name":"CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhan","family":"Jie","sequence":"additional","affiliation":[{"name":"Guangdong ACXEL Micro & Nano Tech Co., Ltd., Foshan 528000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Jin","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Zhang","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianjie","family":"Guo","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7370-2113","authenticated-orcid":false,"given":"Qi","family":"Huang","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Mei","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuqiang","family":"Ma","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7629-2287","authenticated-orcid":false,"given":"Hanbin","family":"Ma","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China"},{"name":"Guangdong ACXEL Micro & Nano Tech Co., Ltd., Foshan 528000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1038\/nature13118","article-title":"The present and future role of microfluidics in biomedical research","volume":"507","author":"Sackmann","year":"2014","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"11196","DOI":"10.1021\/acsomega.0c01274","article-title":"Integrated Digital Microfluidic Platform for Colorimetric Sensing of Nitrite","volume":"5","author":"Gu","year":"2020","journal-title":"ACS Omega"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-020-18575-6","article-title":"Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay","volume":"11","author":"Ding","year":"2020","journal-title":"Nat. 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