{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T18:54:36Z","timestamp":1789412076437,"version":"build-2803163510"},"reference-count":42,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T00:00:00Z","timestamp":1655942400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009553","name":"the Czech Health Research Council","doi-asserted-by":"publisher","award":["NU20-06-0024"],"award-info":[{"award-number":["NU20-06-0024"]}],"id":[{"id":"10.13039\/501100009553","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009553","name":"the Czech Health Research Council","doi-asserted-by":"publisher","award":["21-01090S"],"award-info":[{"award-number":["21-01090S"]}],"id":[{"id":"10.13039\/501100009553","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"the Czech Science Foundation","doi-asserted-by":"publisher","award":["NU20-06-0024"],"award-info":[{"award-number":["NU20-06-0024"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"the Czech Science Foundation","doi-asserted-by":"publisher","award":["21-01090S"],"award-info":[{"award-number":["21-01090S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>We constructed a sensor for the determination of Fe2+ and\/or Fe3+ ions that consists of a polyaniline layer as an ion-to-electron transducer; on top of it, chelating molecules are deposited (which can selectively chelate specific ions) and protected with a non-biofouling poly(2-methyl-2-oxazoline)s layer. We have shown that our potentiometric sensing layers show a rapid response to the presence of Fe2+ or Fe3+ ions, do not experience interference with other ions (such as Cu2+), and work in a biological environment in the presence of bovine serum albumin (as a model serum protein). The sensing layers detect iron ions in the concentration range from 5 nM to 50 \u00b5M.<\/jats:p>","DOI":"10.3390\/bios12070446","type":"journal-article","created":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T21:25:00Z","timestamp":1656019500000},"page":"446","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Potentiometric Performance of Ion-Selective Electrodes Based on Polyaniline and Chelating Agents: Detection of Fe2+ or Fe3+ Ions"],"prefix":"10.3390","volume":"12","author":[{"given":"Rimeh","family":"Ismail","sequence":"first","affiliation":[{"name":"Institute of Macromolecular Chemistry AS CR, Heyrovsk\u00e9ho N\u00e1m. 2, 162 06 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"\u0160ed\u011bnkov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Macromolecular Chemistry AS CR, Heyrovsk\u00e9ho N\u00e1m. 2, 162 06 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zulfiya","family":"\u010cernochov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Macromolecular Chemistry AS CR, Heyrovsk\u00e9ho N\u00e1m. 2, 162 06 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iryna","family":"Romanenko","sequence":"additional","affiliation":[{"name":"Institute of Macromolecular Chemistry AS CR, Heyrovsk\u00e9ho N\u00e1m. 2, 162 06 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7938-9271","authenticated-orcid":false,"given":"Ognen","family":"Pop-Georgievski","sequence":"additional","affiliation":[{"name":"Institute of Macromolecular Chemistry AS CR, Heyrovsk\u00e9ho N\u00e1m. 2, 162 06 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5075-261X","authenticated-orcid":false,"given":"Martin","family":"Hrub\u00fd","sequence":"additional","affiliation":[{"name":"Institute of Macromolecular Chemistry AS CR, Heyrovsk\u00e9ho N\u00e1m. 2, 162 06 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1749-1091","authenticated-orcid":false,"given":"Elena","family":"Tom\u0161\u00edk","sequence":"additional","affiliation":[{"name":"Institute of Macromolecular Chemistry AS CR, Heyrovsk\u00e9ho N\u00e1m. 2, 162 06 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"113185","DOI":"10.1016\/j.molliq.2020.113185","article-title":"An amplified voltammetric sensor based on platinum nanoparticle\/polyoxometalate\/two-dimensional hexagonal boron nitride nanosheets composite and ionic liquid for determination of N-hydroxysuccinimide in water samples","volume":"310","author":"Karimi","year":"2020","journal-title":"J. Mol. Liq."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"111931","DOI":"10.1016\/j.fct.2020.111931","article-title":"A review on graphene-based electrochemical sensor for mycotoxins detection","volume":"148","author":"Le","year":"2021","journal-title":"Food Chem. Toxicol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"104615","DOI":"10.1016\/j.ultsonch.2019.104615","article-title":"Ultrasonic-Assisted Synthesis and In Vitro Biological Assessments of a Novel Herceptin-Stabilized Graphene using Three Dimensional Cell Spheroid","volume":"58","author":"Askari","year":"2019","journal-title":"Ultrason. Sonochem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1016\/j.msec.2018.12.140","article-title":"A Comparative Study on Biological Properties of Novel Nanostructured Monticellite-Based Composites with Hydroxyapatite Bioceramic","volume":"98","author":"Kalantari","year":"2019","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"15342","DOI":"10.1039\/C6RA24760A","article-title":"Polyaniline nanoflowers grafted onto nanodiamonds via a soft template-guided secondary nucleation process for high-performance glucose sensing","volume":"7","author":"Komathi","year":"2017","journal-title":"RSC Adv."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2138","DOI":"10.1039\/C7RA09097H","article-title":"Highly selective non-enzymatic electrochemical sensor based on a titanium dioxide nanowire\u2013poly (3-aminophenyl boronic acid)\u2013gold nanoparticle ternary nanocomposite","volume":"8","author":"Muthuchamy","year":"2018","journal-title":"RSC Adv."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.bios.2016.06.005","article-title":"Enhanced photoelectrochemical biosensing performances for graphene (2D)\u2013Titanium dioxide nanowire (1D) heterojunction polymer conductive nanosponges","volume":"89","author":"Muthuchamy","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3201","DOI":"10.1039\/C6CC08822H","article-title":"Electrochemical synthesis of polyaniline in the micropores of activated carbon for high-performance electrochemical capacitors","volume":"53","author":"Itoi","year":"2017","journal-title":"Chem. Commun."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2178","DOI":"10.1021\/ma902317k","article-title":"Electrochemical Synthesis of Polyaniline Nanobelts with Predominant Electrochemical Performances","volume":"43","author":"Li","year":"2010","journal-title":"Macromolecules"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"21261","DOI":"10.1021\/acsami.6b03463","article-title":"Supercapacitor Electrodes Based on High-Purity Electrospun Polyaniline and Polyaniline\u2212Carbon Nanotube Nanofibers","volume":"8","author":"Simotwo","year":"2016","journal-title":"CS Appl. Mater. Interfaces"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"112099","DOI":"10.1016\/j.fct.2021.112099","article-title":"A nvel non-enzymatic glucose sensor based on NiFe(NPs)-polyaniline hybrid materials","volume":"151","author":"Lakhdari","year":"2021","journal-title":"Food Chem. Toxicol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"136348","DOI":"10.1016\/j.electacta.2020.136348","article-title":"Enhanced electrochemical performance of polyaniline-based electrode for supercapacitors in mixed aqueous electrolyte","volume":"349","author":"Wang","year":"2020","journal-title":"Electrochim. Acta"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"136039","DOI":"10.1016\/j.electacta.2020.136039","article-title":"Co-MOF\/polyanilinebased electrode material for high performance asymmetric supercapacitor devices","volume":"346","author":"Iqbal","year":"2020","journal-title":"Electrochim. Acta"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"11316","DOI":"10.1038\/s41598-017-11831-8","article-title":"Fabrication and characterisation of magnetic graphene oxide Incorporated Fe3O4 @polyaniline for the removal of Bisphenol A, t-octyl-phenol and \u03b1-naphthol from water","volume":"7","author":"Zhou","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"7490","DOI":"10.1007\/s11356-017-8465-z","article-title":"Removol of elevated level of chromium in Groundwater by the fabricated PANI\/Fe3O4 nanocomposites","volume":"24","author":"Ramachandran","year":"2017","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1007\/s13726-020-00822-8","article-title":"Efcient room temperature methanol sensors based on polyaniline\/ graphene micro\/nanocomposites","volume":"29","author":"Pal","year":"2020","journal-title":"Iran. Polym. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.aca.2020.09.004","article-title":"Fe2O3\/polyaniline supramolecular nanocomposite: A receptor free sensor platform for the quantitative determination of serum creatinine","volume":"1137","author":"Sriramprabha","year":"2020","journal-title":"Anal. Chim. Acta"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"18852","DOI":"10.1039\/D1RA02325J","article-title":"Electrochemical deposition of highly hydrophobic perfluorinated polyaniline film for biosensor applications","volume":"11","author":"Dallas","year":"2021","journal-title":"RSC Adv."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"100479","DOI":"10.1016\/j.mtchem.2021.100479","article-title":"Sacrificial template synthesis of ultrathin polyaniline nanosheets and their application in highly sensitive electrochemical dopamine detection","volume":"20","author":"Chen","year":"2021","journal-title":"Mater. Today Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1016\/j.talanta.2004.05.005","article-title":"A novel ion selective membrane potentiometric sensor for direct determination of Fe(III) in the presence of Fe(II)","volume":"64","author":"Mashhadizadeh","year":"2004","journal-title":"Talanta"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1002\/elan.200302956","article-title":"Chromium(III) Ion Selective Electrode Based on Oxalic AcidBis(Cyclohexylidene Hydrazide","volume":"16","author":"Gholivand","year":"2004","journal-title":"Electroanalysis"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/S0039-9140(03)00130-9","article-title":"Chromium(III) ion selective electrode based on glyoxal bis(2-hydroxyanil)","volume":"60","author":"Gholivand","year":"2003","journal-title":"Talanta"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.1021\/ac9507000","article-title":"PVC-Based 2,2,2-Cryptand Sensor for Zinc Ions","volume":"68","author":"Srivastava","year":"1996","journal-title":"Anal. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1016\/j.msec.2010.11.016","article-title":"Neodymium(III)\u2013PVC membrane sensor based on a new four dentate ionophore","volume":"31","author":"Zamani","year":"2011","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1016\/j.msec.2012.10.004","article-title":"Dysprosium selective potentiometric membrane sensor","volume":"33","author":"Zamani","year":"2013","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1002\/elan.200603768","article-title":"Highly Selective and Sensitive Membrane Sensors for Copper(II)Ion Based on a New Benzo-Substituted Macrocyclic Diamide 6,7,8,9,10-Hexahydro-2H-1,13,4,7,10-benzodioxatriazacyclopenta-decine-3,11(4H,12H)-dione","volume":"19","author":"Shamsipur","year":"2007","journal-title":"Electroanalysis"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1002\/elan.202060319","article-title":"Novel Potentiometric Solid-contact Electrode for the Determination of Fe2+ Ions via MWCNTs-Gemifloxacin Composite","volume":"33","author":"Abdallah","year":"2021","journal-title":"Electroanalysis"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2394","DOI":"10.1016\/j.jiec.2013.10.019","article-title":"Potentiometric determination of iron in polluted water samples using new modified Fe(III)-screen printed ion selective electrode","volume":"20","author":"Ali","year":"2014","journal-title":"J. Ind. Eng. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"10473","DOI":"10.1016\/S1452-3981(23)13123-3","article-title":"A Novel Iron(III) Selective Potentiometric Sensor Based on 9- Ethylacenaphtho [1, 2-B] Quinoxaline","volume":"8","author":"Mizani","year":"2013","journal-title":"Int. J. Electrochem. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.biomaterials.2018.05.022","article-title":"Poly(2-oxazoline)s based biomaterials: A comprehensive and critical update","volume":"178","author":"Lorson","year":"2018","journal-title":"Biomaterials"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2874","DOI":"10.1039\/D0BM02217A","article-title":"The role of poly(2-alkyl-2-oxazoline)s in hydrogels and biofabrication","volume":"9","author":"Trachsel","year":"2021","journal-title":"Biomater. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.1016\/j.addr.2007.08.018","article-title":"Poly(2-oxazolines) in biological and biomedical application contexts","volume":"59","author":"Adams","year":"2007","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"14323","DOI":"10.1021\/ja504390x","article-title":"Therapeutic protein-polymer conjugates: Advancing beyond PEGylation","volume":"136","author":"Lin","year":"2014","journal-title":"J. Am. Chem. Soc."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"131827","DOI":"10.1016\/j.snb.2022.131827","article-title":"Selective potentiometric detection of reactive oxygen species (ROS) in biologically relevant concentrations by a modified metalized polyporphyrine sensing layer coated with nonbiofouling poly(2-alkyl-2oxazoline)s","volume":"363","author":"Ivanko","year":"2022","journal-title":"Sens. Actuators B Chem."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Hruby, M., Martinez, I.V.S., Stephan, H., Pouckova, P., Benes, J., and Stepanek, P. (2021). Chelators for Treatment of Iron and Copper Overload: Shift from Low-Molecular-Weight Compounds to Polymers. Polymer, 13.","DOI":"10.3390\/polym13223969"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"4718","DOI":"10.1039\/D1CC00642H","article-title":"Antifouling fluoropolymer-coated nanomaterials for 19F MRI","volume":"57","author":"Jirak","year":"2021","journal-title":"Chem. Comm."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3232","DOI":"10.1021\/bm2007086","article-title":"Poly(ethylene oxide) Layers Grafted to Dopamine-melanin Anchoring Layer: Stability and Resistance to Protein Adsorption","volume":"12","author":"Houska","year":"2011","journal-title":"Biomacromolecules"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/S0040-6090(03)00279-7","article-title":"Spectroscopic ellipsometry of electrochemically prepared thin filmpolyaniline","volume":"429","author":"Monkman","year":"2003","journal-title":"Thin Solid Film."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"8022","DOI":"10.1021\/acs.jpcc.8b01948","article-title":"Assembly and Interaction of Polyaniline Chains: Impact on Electro- and Physical\u2013Chemical Behavior","volume":"122","author":"Tomsik","year":"2018","journal-title":"J. Phys. Chem. C"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1016\/j.electacta.2005.04.074","article-title":"Preparation and cyclic voltammetry studies on nickel-nanoclusters containing polyaniline composites having layer-by-layer structures","volume":"51","author":"Trung","year":"2005","journal-title":"Electrochim. Acta"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"25557","DOI":"10.1016\/S0021-9258(18)47285-7","article-title":"Zinc (II) and Copper (II) Binding to Serum Albumin","volume":"269","author":"Masuoka","year":"1994","journal-title":"J. Biol. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.foodchem.2013.07.143","article-title":"Serum albumin forms a lactoferrin-like soluble iron-binding complex in presence of hydrogen carbonate ions","volume":"145","author":"Ueno","year":"2014","journal-title":"Food Chem."}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/12\/7\/446\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:38:51Z","timestamp":1760139531000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/12\/7\/446"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,23]]},"references-count":42,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["bios12070446"],"URL":"https:\/\/doi.org\/10.3390\/bios12070446","relation":{},"ISSN":["2079-6374"],"issn-type":[{"value":"2079-6374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,23]]}}}