{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:13:53Z","timestamp":1790291633288,"version":"4.1.0"},"reference-count":33,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T00:00:00Z","timestamp":1660089600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31872909"],"award-info":[{"award-number":["31872909"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32111530291"],"award-info":[{"award-number":["32111530291"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32001781"],"award-info":[{"award-number":["32001781"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["ZR2020QC249"],"award-info":[{"award-number":["ZR2020QC249"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Shandong Provincial Natural Science Foundation","doi-asserted-by":"publisher","award":["31872909"],"award-info":[{"award-number":["31872909"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Shandong Provincial Natural Science Foundation","doi-asserted-by":"publisher","award":["32111530291"],"award-info":[{"award-number":["32111530291"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Shandong Provincial Natural Science Foundation","doi-asserted-by":"publisher","award":["32001781"],"award-info":[{"award-number":["32001781"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Shandong Provincial Natural Science Foundation","doi-asserted-by":"publisher","award":["ZR2020QC249"],"award-info":[{"award-number":["ZR2020QC249"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Herein, a novel electrochemical aptasensor using a broad-spectrum aptamer as a biorecognition element was constructed based on a screen-printed carbon electrode (SPCE) for simultaneous detection of aminoglycoside antibiotics (AAs). The ordered mesoporous carbon (OMC) was firstly modified on 2D Ti3C2 MXene. The addition of OMC not only effectively improved the stability of the aptasensor, but also prevented the stacking of Ti3C2 sheets, which formed a good current passage for signal amplification. The prepared OMC@Ti3C2 MXene functioned as a nanocarrier to accommodate considerable aptamers. In the presence of AAs, the transport of electron charge on SPCE surface was influenced by the bio-chemical reactions of the aptamer and AAs, generating a significant decline in the differential pulse voltammetry (DPV) signals. The proposed aptasensor presented a wide linear range and the detection limit was 3.51 nM. Moreover, the aptasensor, with satisfactory stability, reproducibility and specificity, was successfully employed to detect the multi-residuals of AAs in milk. This work provided a novel strategy for monitoring AAs in milk.<\/jats:p>","DOI":"10.3390\/bios12080626","type":"journal-article","created":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T01:58:30Z","timestamp":1660183110000},"page":"626","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Novel Electrochemical Aptasensor Based on Ordered Mesoporous Carbon\/2D Ti3C2 MXene as Nanocarrier for Simultaneous Detection of Aminoglycoside Antibiotics in Milk"],"prefix":"10.3390","volume":"12","author":[{"given":"Fengling","family":"Yue","sequence":"first","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyue","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyuan","family":"Bai","sequence":"additional","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjiao","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Falan","family":"Li","sequence":"additional","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, China"},{"name":"Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, Zibo 255049, China"},{"name":"Zibo City Key Laboratory of Agricultural Product Safety Traceability, Zibo 255049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yemin","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, China"},{"name":"Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, Zibo 255049, China"},{"name":"Zibo City Key Laboratory of Agricultural Product Safety Traceability, Zibo 255049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6796-8994","authenticated-orcid":false,"given":"Igor","family":"Vrublevsky","sequence":"additional","affiliation":[{"name":"Department of Information Security, Belarusian State University of Informatics and Radioelectronics, 220013 Minsk, Belarus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, China"},{"name":"Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, Zibo 255049, China"},{"name":"Zibo City Key Laboratory of Agricultural Product Safety Traceability, Zibo 255049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"338569","DOI":"10.1016\/j.aca.2021.338569","article-title":"Electrochemical methods for the determination of antibiotic residues in milk: A critical review","volume":"1173","author":"Lisboa","year":"2021","journal-title":"Anal. Chim. Acta"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"127218","DOI":"10.1016\/j.bmcl.2020.127218","article-title":"The relationship between the structure and toxicity of aminoglycoside antibiotics","volume":"30","author":"Siomin","year":"2020","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3220","DOI":"10.1016\/j.sapharm.2021.10.004","article-title":"A scoping review of global aminoglycoside antibiotic overuse: A potential opportunity for primary ototoxicity prevention","volume":"18","author":"Dillard","year":"2022","journal-title":"Res. Soc. Adm. Pharm."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"6595","DOI":"10.1021\/ac300815c","article-title":"Toggled RNA Aptamers Against Aminoglycosides Allowing Facile Detection of Antibiotics Using Gold Nanoparticle Assays","volume":"84","author":"Derbyshire","year":"2012","journal-title":"Anal. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.jinf.2021.05.010","article-title":"Prevalence of aminoglycoside-induced hearing loss in drug-resistant tuberculosis patients: A systematic review","volume":"83","author":"Dillard","year":"2021","journal-title":"J. Infect."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"101525","DOI":"10.1016\/j.tice.2021.101525","article-title":"In vivo assessment of reversing aminoglycoside antibiotics nephrotoxicity using Jatropha mollissima crude extract","volume":"72","author":"Iqbal","year":"2021","journal-title":"Tissue Cell"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.bios.2017.12.006","article-title":"Evanescent wave aptasensor for continuous and online aminoglycoside antibiotics detection based on target binding facilitated fluorescence quenching","volume":"102","author":"Tang","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.chroma.2016.02.003","article-title":"Study of matrix effects for liquid chromatography\u2013electrospray ionization tandem mass spectrometric analysis of 4 aminoglycosides residues in milk","volume":"1437","author":"Wang","year":"2016","journal-title":"J. Chromatogr. A"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"108374","DOI":"10.1016\/j.foodcont.2021.108374","article-title":"Multi-residue determination of twenty aminoglycoside antibiotics in various food matrices by dispersive solid phase extraction and liquid chromatography-tandem mass spectrometry","volume":"130","author":"Kim","year":"2021","journal-title":"Food Control"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"462133","DOI":"10.1016\/j.chroma.2021.462133","article-title":"Aminoglycosides analysis optimization using ion pairing liquid chromatography coupled to tandem mass spectrometry and application on wastewater samples","volume":"1651","author":"Guironnet","year":"2021","journal-title":"J. Chromatogr. A"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.chroma.2018.02.029","article-title":"Optimization and application of parallel solid-phase extraction coupled with ultra-high performance liquid chromatography\u2013tandem mass spectrometry for the determination of 11 aminoglycoside residues in honey and royal jelly","volume":"1542","author":"Wang","year":"2018","journal-title":"J. Chromatogr. A"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"116034","DOI":"10.1016\/j.trac.2020.116034","article-title":"Determination of aminoglycoside antibiotics: Current status and future trends","volume":"131","author":"Glinka","year":"2020","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.jpba.2018.06.033","article-title":"Recent advances in the capillary electrophoresis analysis of antibiotics with capacitively coupled contactless conductivity detection","volume":"158","author":"Paul","year":"2018","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1126\/science.2200121","article-title":"Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase","volume":"249","author":"Tuerk","year":"1990","journal-title":"Science"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1038\/346818a0","article-title":"In vitro selection of RNA molecules that bind specific ligands","volume":"346","author":"Ellington","year":"1990","journal-title":"Nature"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"129956","DOI":"10.1016\/j.snb.2021.129956","article-title":"High-affinity aptamer of allergen \u03b2-lactoglobulin: Selection, recognition mechanism and application","volume":"340","author":"Qi","year":"2021","journal-title":"Sens. Actuators B Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1021\/acssensors.8b00516","article-title":"Strategies for Creating Structure-Switching Aptamers","volume":"3","author":"Feagin","year":"2018","journal-title":"ACS Sens."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"129607","DOI":"10.1016\/j.snb.2021.129607","article-title":"Aptamer-functionalized Au nanoparticles array as the effective SERS biosensor for label-free detection of interleukin-6 in serum","volume":"334","author":"Muhammad","year":"2021","journal-title":"Sens. Actuators B Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"130959","DOI":"10.1016\/j.snb.2021.130959","article-title":"Selection of broad-spectrum aptamer and its application in fabrication of aptasensor for detection of aminoglycoside antibiotics residues in milk","volume":"351","author":"Yue","year":"2022","journal-title":"Sens. Actuators B Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"130812","DOI":"10.1016\/j.snb.2021.130812","article-title":"Multi-target electrochemical malaria aptasensor on flexible multielectrode arrays for detection in malaria parasite blood samples","volume":"349","author":"Chen","year":"2021","journal-title":"Sens. Actuators B Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"112598","DOI":"10.1016\/j.bios.2020.112598","article-title":"Highly sensitive label-free electrochemical aptasensors based on photoresist derived carbon for cancer biomarker detection","volume":"170","author":"Forouzanfar","year":"2020","journal-title":"Biosens. Bioelectron."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"113247","DOI":"10.1016\/j.bios.2021.113247","article-title":"Electrochemical aptasensor for 17\u03b2-estradiol using disposable laser scribed graphene electrodes","volume":"185","author":"Chang","year":"2021","journal-title":"Biosens. Bioelectron."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"143129","DOI":"10.1016\/j.scitotenv.2020.143129","article-title":"Recent advances in aptamer-based sensors for aminoglycoside antibiotics detection and their applications","volume":"762","author":"Yue","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"113617","DOI":"10.1016\/j.bios.2021.113617","article-title":"Nanomaterial-based aptasensors as an efficient substitute for cardiovascular disease diagnosis: Future of smart biosensors","volume":"193","author":"Azzouz","year":"2021","journal-title":"Biosens. Bioelectron."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"122866","DOI":"10.1016\/j.talanta.2021.122866","article-title":"Signal enhancing strategies in aptasensors for the detection of small molecular contaminants by nanomaterials and nucleic acid amplification","volume":"236","author":"Zhang","year":"2022","journal-title":"Talanta"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"111933","DOI":"10.1016\/j.bios.2019.111933","article-title":"Detection of pathogenic bacteria via nanomaterials-modified aptasensors","volume":"150","author":"Sharifi","year":"2020","journal-title":"Biosens. Bioelectron."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"113869","DOI":"10.1016\/j.jelechem.2020.113869","article-title":"Hierarchical Ti3C2 MXene-derived sodium titanate nanoribbons\/PEDOT for signal amplified electrochemical immunoassay of prostate specific antigen","volume":"860","author":"Xu","year":"2020","journal-title":"J. Electroanal. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"107674","DOI":"10.1016\/j.bioelechem.2020.107674","article-title":"A MoS2@Ti3C2Tx MXene hybrid-based electrochemical aptasensor (MEA) for sensitive and rapid detection of Thyroxine","volume":"137","author":"Koyappayil","year":"2021","journal-title":"Bioelectrochemistry"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"121879","DOI":"10.1016\/j.talanta.2020.121879","article-title":"Ti3C2 MXene mediated Prussian blue in situ hybridization and electrochemical signal amplification for the detection of exosomes","volume":"224","author":"Zhang","year":"2021","journal-title":"Talanta"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.bios.2018.10.016","article-title":"Ti3C2 MXenes nanosheets catalyzed highly efficient electrogenerated chemiluminescence biosensor for the detection of exosomes","volume":"124\u2013125","author":"Zhang","year":"2019","journal-title":"Biosens. Bioelectron."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.snb.2018.03.042","article-title":"Multiplex electrochemical aptasensor for detecting multiple antibiotics residues based on carbon fiber and mesoporous carbon-gold nanoparticles","volume":"265","author":"Li","year":"2018","journal-title":"Sens. Actuators B Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"7087","DOI":"10.1021\/acsami.8b21117","article-title":"Understanding the Different Diffusion Mechanisms of Hydrated Protons and Potassium Ions in Titanium Carbide MXene","volume":"11","author":"Wen","year":"2019","journal-title":"Acs Appl. Mater. Inter."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.bios.2015.03.028","article-title":"Label-free impedimetric aptasensor for detection of femtomole level acetamiprid using gold nanoparticles decorated multiwalled carbon nanotube-reduced graphene oxide nanoribbon composites","volume":"70","author":"Fei","year":"2015","journal-title":"Biosens. Bioelectron."}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/12\/8\/626\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:06:59Z","timestamp":1760141219000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/12\/8\/626"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,10]]},"references-count":33,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["bios12080626"],"URL":"https:\/\/doi.org\/10.3390\/bios12080626","relation":{},"ISSN":["2079-6374"],"issn-type":[{"value":"2079-6374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,10]]}}}