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for point-of-care (POC) testing with minimal user training and technical requirements. However, most \u03bcPADs use dried bioreagents, which complicate production, reduce device reproducibility and stability, and require transport and storage under temperature and humidity-controlled conditions. In this work, we propose a \u03bcPAD produced using an affordable craft-cutter and stored at room temperature, which is used to partially automate a single-step colorimetric magneto-immunoassay. As a proof-of-concept, the \u03bcPAD has been applied to the quantitative detection of Plasmodium falciparum lactate dehydrogenase (Pf-LDH), a biomarker of malaria infection. In this system, detection is based on a single-step magneto-immunoassay that consists of a single 5-min incubation of the lysed blood sample with immuno-modified magnetic beads (MB), detection antibody, and an enzymatic signal amplifier (Poly-HRP). This mixture is then transferred to a single-piece paper device where, after on-chip MB magnetic concentration and washing, signal generation is achieved by adding a chromogenic enzyme substrate. The colorimetric readout is achieved by the naked eye or using a smartphone camera and free software for image analysis. This \u03bcPAD afforded quantitative Pf-LDH detection in &lt;15 min, with a detection limit of 6.25 ng mL\u22121 when the result was interpreted by the naked eye and 1.4 ng mL\u22121 when analysed using the smartphone imaging system. Moreover, the study of a battery of clinical samples revealed concentrations of Pf-LDH that correlated with those provided by the reference ELISA and with better sensitivity than a commercial rapid diagnostic test (RDT). These results demonstrate that magneto-immunoassays can be partly automated by employing a \u03bcPAD, achieving a level of handling that approaches the requirements of POC testing.<\/jats:p>","DOI":"10.3390\/bios12090680","type":"journal-article","created":{"date-parts":[[2022,8,25]],"date-time":"2022-08-25T21:28:12Z","timestamp":1661462892000},"page":"680","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Magnetic Bead Handling Using a Paper-Based Device for Quantitative Point-of-Care Testing"],"prefix":"10.3390","volume":"12","author":[{"given":"Kevin","family":"Arias-Alp\u00edzar","sequence":"first","affiliation":[{"name":"Diagnostic Nanotools Group, Vall d\u2019Hebron Institut de Recerca (VHIR), 08035 Barcelona, Spain"},{"name":"Universitat Aut\u00f2noma de Barcelona (UAB), 08193 Barcelona, 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Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, 28028 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7435-1829","authenticated-orcid":false,"given":"Pau","family":"Bosch-Nicolau","sequence":"additional","affiliation":[{"name":"International Health Unit Vall d\u2019Hebron-Drassanes, Vall d\u2019Hebron Hospital Universitari, PROSICS Barcelona, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, 28028 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Salvador","sequence":"additional","affiliation":[{"name":"International Health Unit Vall d\u2019Hebron-Drassanes, Vall d\u2019Hebron Hospital Universitari, PROSICS Barcelona, 08035 Barcelona, Spain"},{"name":"Centro de 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