{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T06:17:18Z","timestamp":1762064238394,"version":"build-2065373602"},"reference-count":22,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key R&amp;D Program of China","doi-asserted-by":"publisher","award":["2021YFA1301200","81871662","2021ZDLSF01-10","2019ZDLSF03-02","XJTU1AF-CRF-2018-009"],"award-info":[{"award-number":["2021YFA1301200","81871662","2021ZDLSF01-10","2019ZDLSF03-02","XJTU1AF-CRF-2018-009"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2021YFA1301200","81871662","2021ZDLSF01-10","2019ZDLSF03-02","XJTU1AF-CRF-2018-009"],"award-info":[{"award-number":["2021YFA1301200","81871662","2021ZDLSF01-10","2019ZDLSF03-02","XJTU1AF-CRF-2018-009"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key research and development plan of Shaanxi Province","award":["2021YFA1301200","81871662","2021ZDLSF01-10","2019ZDLSF03-02","XJTU1AF-CRF-2018-009"],"award-info":[{"award-number":["2021YFA1301200","81871662","2021ZDLSF01-10","2019ZDLSF03-02","XJTU1AF-CRF-2018-009"]}]},{"name":"Clinical Research Award of the First Affiliated Hospital of Xi\u2019an Jiaotong University, China","award":["2021YFA1301200","81871662","2021ZDLSF01-10","2019ZDLSF03-02","XJTU1AF-CRF-2018-009"],"award-info":[{"award-number":["2021YFA1301200","81871662","2021ZDLSF01-10","2019ZDLSF03-02","XJTU1AF-CRF-2018-009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Blood iron levels play a vital role in oxygen metabolism and energy generation whilst transporter protein, transferrin, binds and delivers iron to the transferrin receptor of endosomal compartments of cells. Consequently, the iron-binding capacity of transferrin is an important indicator for many diseases, and its measurements are used in the diagnosis and treatment of anaemias. Various assays, including Total Iron Binding Capacity (TIBC), Unsaturated Iron-Binding Capacity (UIBC) and Transferrin Saturation (TS), were developed to assess the iron-binding capacity of transferrin. Clinically, UIBC is measured in serum by a multi-step liquid ferrozine method and subjected to interference from conditions such as haemolysis and lipemia. Here, we report a quick method that directly measures the concentration of apotransferrin in EDTA-treated plasma, theoretically equivalent to UIBC. Importantly, this supramolecular assembly-based method is more time-efficient, cost-effective and insensitive to serum cation fluctuations. With additional colorimetric property, this method also provides a visual indicator for abnormal health conditions with extreme transferrin statuses such as those found in cancers. Its minimal requirement for equipment would be particularly useful for diagnosis in remote and under-developed regions.<\/jats:p>","DOI":"10.3390\/bios12090708","type":"journal-article","created":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T21:27:17Z","timestamp":1662067637000},"page":"708","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["An Improved Method for Quick Quantification of Unsaturated Transferrin"],"prefix":"10.3390","volume":"12","author":[{"given":"Ruirui","family":"Guo","sequence":"first","affiliation":[{"name":"BioBank, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juanjuan","family":"Gao","sequence":"additional","affiliation":[{"name":"BioBank, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingyun","family":"Hui","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanqing","family":"Li","sequence":"additional","affiliation":[{"name":"BioBank, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junhui","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Fu","sequence":"additional","affiliation":[{"name":"College of Chemical Engineering, North China University of Science and Technology, Tangshan 063210, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Chemical Engineering, North China University of Science and Technology, Tangshan 063210, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yawen","family":"Wang","sequence":"additional","affiliation":[{"name":"BioBank, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"},{"name":"Department of Laboratory Medicine, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1670-6115","authenticated-orcid":false,"given":"Bing","family":"Liu","sequence":"additional","affiliation":[{"name":"BioBank, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"},{"name":"Department of Laboratory Medicine, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,1]]},"reference":[{"key":"ref_1","first-page":"164","article-title":"Review on iron and its importance for human health","volume":"19","author":"Abbaspour","year":"2014","journal-title":"J. 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