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Therefore, it is of great significance to realize the reversible monitoring of them at the intracellular level. Here, we synthesized a reversible ratiometric fluorescent probe through a reasonable design, which can sensitively monitor SO2 derivatives and FA, and the detection limit can reach 0.16 \u03bcM. The probe can specifically target mitochondria and successfully monitor the fluctuations of SO2 and FA in living cells. It also works well in the detection of SO2 and FA in zebrafish. This high-performance probe is expected to find broad in vitro and in vivo applications.<\/jats:p>","DOI":"10.3390\/bios12090715","type":"journal-article","created":{"date-parts":[[2022,9,5]],"date-time":"2022-09-05T20:48:25Z","timestamp":1662410905000},"page":"715","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Rational Construction of a Mitochondria-Targeted Reversible Fluorescent Probe with Intramolecular FRET for Ratiometric Monitoring Sulfur Dioxide and Formaldehyde"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3021-227X","authenticated-orcid":false,"given":"Jinxiao","family":"Lyu","sequence":"first","affiliation":[{"name":"Cancer Centre and Centre of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, Macau SAR 999078, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunfei","family":"Wang","sequence":"additional","affiliation":[{"name":"Cancer Centre and Centre of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, Macau SAR 999078, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6452-1778","authenticated-orcid":false,"given":"Xuanjun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Cancer Centre and Centre of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, Macau SAR 999078, China"},{"name":"MOE Frontiers Science Center for Precision Oncology, University of Macau, Macau SAR 999078, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"101867","DOI":"10.1016\/j.redox.2021.101867","article-title":"Oxidative Eustress: On Constant Alert for Redox Homeostasis","volume":"41","author":"Sies","year":"2021","journal-title":"Redox Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1080\/10715762.2019.1612059","article-title":"Oxidative Stress in Biological Systems and Its Relation with Pathophysiological Functions: The Effect of Physical Activity on Cellular Redox Homeostasis","volume":"53","author":"Kruk","year":"2019","journal-title":"Free Radic. 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