{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T01:45:13Z","timestamp":1781833513867,"version":"3.54.5"},"reference-count":48,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T00:00:00Z","timestamp":1663545600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the RSCF (Russian Scientific Foundation)","award":["20-73-10186"],"award-info":[{"award-number":["20-73-10186"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Dyad compound NI-SP bearing 1,8-naphthalimide (NI) and styrylpyridine (SP) photoactive units, in which the N-phenylazadithia-15-crown-5 ether receptor is linked with the energy donor naphthalimide chromophore, has been evaluated as a ratiometric fluorescent chemosensor for mercury (II) ions in living cells. In an aqueous solution, NI-SP selectively responds to the presence of Hg2+ via the enhancement in the emission intensity of NI due to the inhibition of the photoinduced electron transfer from the receptor to the NI fragment. At the same time, the long wavelength fluorescence band of SP, arising as a result of resonance energy transfer from the excited NI unit, appears to be virtually unchanged upon Hg2+ binding. This allows self-calibration of the optical response. The observed spectral behavior is consistent with the formation of the (NI-SP)\u00b7Hg2+ complex (dissociation constant 0.13 \u00b1 0.04 \u00b5M). Bio-imaging studies showed that the ratio of fluorescence intensity in the 440\u2013510 nm spectral region to that in the 590\u2013650 nm region increases from 1.1 to 2.8 when cells are exposed to an increasing concentration of mercury (II) ions, thus enabling the detection of intracellular Hg2+ ions and their quantitative analysis in the 0.04\u20131.65 \u03bcM concentration range.<\/jats:p>","DOI":"10.3390\/bios12090770","type":"journal-article","created":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T21:47:27Z","timestamp":1663624047000},"page":"770","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Fluorescent RET-Based Chemosensor Bearing 1,8-Naphthalimide and Styrylpyridine Chromophores for Ratiometric Detection of Hg2+ and Its Bio-Application"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8303-0245","authenticated-orcid":false,"given":"Pavel","family":"Panchenko","sequence":"first","affiliation":[{"name":"Laboratory of Photoactive Supramolecular Systems, A. N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences, Vavilova Str. 28, 119991 Moscow, Russia"},{"name":"Department of Technology of Fine Organic Synthesis and Chemistry of Dyes, Dmitry Mendeleev University of Chemical Technology of Russia, Miusskaya Sqr. 9, 125047 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasija","family":"Efremenko","sequence":"additional","affiliation":[{"name":"Biological Faculty, Lomonosov Moscow State University, Leninskie Gory 1, 119992 Moscow, Russia"},{"name":"Laboratory of Optical Microscopy and Spectroscopy, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of Russian Academy of Sciences, Miklukho-Maklaya Str. 16\/10, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Polyakova","sequence":"additional","affiliation":[{"name":"Laboratory of Photoactive Supramolecular Systems, A. N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences, Vavilova Str. 28, 119991 Moscow, Russia"},{"name":"Department of Technology of Fine Organic Synthesis and Chemistry of Dyes, Dmitry Mendeleev University of Chemical Technology of Russia, Miusskaya Sqr. 9, 125047 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1596-9506","authenticated-orcid":false,"given":"Alexey","family":"Feofanov","sequence":"additional","affiliation":[{"name":"Biological Faculty, Lomonosov Moscow State University, Leninskie Gory 1, 119992 Moscow, Russia"},{"name":"Laboratory of Optical Microscopy and Spectroscopy, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of Russian Academy of Sciences, Miklukho-Maklaya Str. 16\/10, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9486-594X","authenticated-orcid":false,"given":"Maria","family":"Ustimova","sequence":"additional","affiliation":[{"name":"Laboratory of Photoactive Supramolecular Systems, A. N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences, Vavilova Str. 28, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri","family":"Fedorov","sequence":"additional","affiliation":[{"name":"Laboratory of Photoactive Supramolecular Systems, A. N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences, Vavilova Str. 28, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Fedorova","sequence":"additional","affiliation":[{"name":"Laboratory of Photoactive Supramolecular Systems, A. N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences, Vavilova Str. 28, 119991 Moscow, Russia"},{"name":"Department of Technology of Fine Organic Synthesis and Chemistry of Dyes, Dmitry Mendeleev University of Chemical Technology of Russia, Miusskaya Sqr. 9, 125047 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1006\/enrs.1998.3832","article-title":"Mercury Levels along the Food Chain and Risk for Exposed Populations","volume":"77","author":"Renzoni","year":"1998","journal-title":"Environ. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1006\/enrs.1998.3828","article-title":"Gold Mining as a Source of Mercury Exposure in the Brazilian Amazon","volume":"77","author":"Malm","year":"1998","journal-title":"Environ. 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