{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T07:41:02Z","timestamp":1778744462893,"version":"3.51.4"},"reference-count":56,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,11,27]],"date-time":"2022-11-27T00:00:00Z","timestamp":1669507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Postdoctoral Science Foundation of Chongqing, China","award":["CSTB2022NSCQ-BHX0687"],"award-info":[{"award-number":["CSTB2022NSCQ-BHX0687"]}]},{"name":"Postdoctoral Science Foundation of Chongqing, China","award":["YGZ2021133"],"award-info":[{"award-number":["YGZ2021133"]}]},{"name":"Chongqing Medical and Pharmaceutical College, China","award":["CSTB2022NSCQ-BHX0687"],"award-info":[{"award-number":["CSTB2022NSCQ-BHX0687"]}]},{"name":"Chongqing Medical and Pharmaceutical College, China","award":["YGZ2021133"],"award-info":[{"award-number":["YGZ2021133"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>In this study, the personal glucose meter (PGM) was first used as a fast and user-friendly meter for analyzing catechol (CA) based on the reduction of the mediator K3[Fe(CN)6] to K4[Fe(CN)6] in the glucose test strip. Then, an easy, low-cost, and convenient PGM-based method for detecting tyrosinase (TYR) activity and sodium benzoate (SBA) was developed on the basis of the TYR-catalyzed reaction. In this method, CA is oxidized to form o-benzoquinone by TYR, thereby reducing the residual amount of CA and the PGM readout. On the other hand, SBA can inhibit the oxidation of CA catalyzed by TYR and increase the residual amount of CA after the enzymatic reaction. Therefore, the activity of TYR is proportional to the difference in the PGM readout of CA, and the concentration of SBA is positively correlated with the residual amount of CA. After the relevant experimental conditions were systematically optimized, the proposed PGM-based method for the detection of TYR and SBA was successfully validated. The liner ranges are 1.0\u2013103.3 U\/mL and 6.25\u20131000 ppm, and the quantification limits are 1.0 U\/mL and 6.25 ppm for TYR and SBA, respectively. Moreover, the spiked recovery tests in normal human serum and carbonate beverages (i.e., Cola, Sprite, and Fanta) were performed, and the recoveries (91.6\u2013106.8%) further confirm the applicability of the PGM-based method in real sample analysis.<\/jats:p>","DOI":"10.3390\/bios12121084","type":"journal-article","created":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T01:58:46Z","timestamp":1669600726000},"page":"1084","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A Catechol-Meter Based on Conventional Personal Glucose Meter for Portable Detection of Tyrosinase and Sodium Benzoate"],"prefix":"10.3390","volume":"12","author":[{"given":"Tao","family":"Tian","sequence":"first","affiliation":[{"name":"Chongqing Key Laboratory of High Active Traditional Chinese Drug Delivery System, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China"},{"name":"School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Yi","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hang-Yu","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-Jing","family":"Peng","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2229-9125","authenticated-orcid":false,"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of High Active Traditional Chinese Drug Delivery System, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9300-5710","authenticated-orcid":false,"given":"Feng-Qing","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Surekha, M., and Reddy, S.M. 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