{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:48:20Z","timestamp":1762080500959,"version":"build-2065373602"},"reference-count":30,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T00:00:00Z","timestamp":1670457600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["31870989","22N31900800"],"award-info":[{"award-number":["31870989","22N31900800"]}]},{"name":"Shanghai Science and Technology Innovation Action Plan in 2022","award":["31870989","22N31900800"],"award-info":[{"award-number":["31870989","22N31900800"]}]},{"name":"Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)","award":["31870989","22N31900800"],"award-info":[{"award-number":["31870989","22N31900800"]}]},{"name":"The Hospital Research Foundation Group","award":["31870989","22N31900800"],"award-info":[{"award-number":["31870989","22N31900800"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Porcine epidemic diarrhea virus (PEDV), a coronavirus that causes highly infectious intestinal diarrhea in piglets, has led to severe economic losses worldwide. Rapid diagnosis and timely supervision are significant in the prophylaxis of PEDV. Herein, we proposed a gold-nanorod (GNR) probe-assisted counting method using dark field microscopy (DFM). The antibody-functionalized silicon chips were prepared to capture PEDV to form sandwich structures with GNR probes for imaging under DFM. Results show that our DFM-based assay for PEDV has a sensitivity of 23.80 copies\/\u03bcL for simulated real samples, which is very close to that of qPCR in this study. This method of GNR probes combined with DFM for quantitative detection of PEDV not only has strong specificity, good repeatability, and a low detection limit, but it also can be implemented for rapid on-site detection of the pathogens.<\/jats:p>","DOI":"10.3390\/bios12121146","type":"journal-article","created":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T02:17:57Z","timestamp":1670465877000},"page":"1146","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Visual and Ultrasensitive Detection of a Coronavirus Using a Gold Nanorod Probe under Dark Field"],"prefix":"10.3390","volume":"12","author":[{"given":"Xuejia","family":"Qian","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Institute of Comparative Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8428-3340","authenticated-orcid":false,"given":"Yuanzhao","family":"Shen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Institute of Comparative Medicine, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiasheng","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Institute of Comparative Medicine, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chih-Tsung","family":"Yang","sequence":"additional","affiliation":[{"name":"Future Industries Institute, Mawson Lakes Campas, University of South Australia, Adelaide, SW 5095, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2515-704X","authenticated-orcid":false,"given":"Xin","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Institute of Comparative Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1128\/JCM.02507-16","article-title":"Reactivity of Porcine Epidemic Diarrhea Virus Structural Proteins to Antibodies against Porcine Enteric Coronaviruses: Diagnostic Implications","volume":"55","author":"Zhang","year":"2017","journal-title":"J. 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