{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T17:19:49Z","timestamp":1782926389465,"version":"3.54.5"},"reference-count":24,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,12,25]],"date-time":"2022-12-25T00:00:00Z","timestamp":1671926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Central Public Interest Scientific Institution Basal Research Fund for the Chinese Academy of Agricultural Sciences","award":["Y2021PT05"],"award-info":[{"award-number":["Y2021PT05"]}]},{"name":"the Central Public Interest Scientific Institution Basal Research Fund for the Chinese Academy of Agricultural Sciences","award":["P42 ES004699"],"award-info":[{"award-number":["P42 ES004699"]}]},{"name":"the Central Public Interest Scientific Institution Basal Research Fund for the Chinese Academy of Agricultural Sciences","award":["2000009144"],"award-info":[{"award-number":["2000009144"]}]},{"name":"National Institute of Environmental Health Science Superfund Research Program","award":["Y2021PT05"],"award-info":[{"award-number":["Y2021PT05"]}]},{"name":"National Institute of Environmental Health Science Superfund Research Program","award":["P42 ES004699"],"award-info":[{"award-number":["P42 ES004699"]}]},{"name":"National Institute of Environmental Health Science Superfund Research Program","award":["2000009144"],"award-info":[{"award-number":["2000009144"]}]},{"name":"National Academy of Sciences","award":["Y2021PT05"],"award-info":[{"award-number":["Y2021PT05"]}]},{"name":"National Academy of Sciences","award":["P42 ES004699"],"award-info":[{"award-number":["P42 ES004699"]}]},{"name":"National Academy of Sciences","award":["2000009144"],"award-info":[{"award-number":["2000009144"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>In this study, a previously unreported 3-((4-(isopropylamino)-6-(methylthio)-1,3,5-triazin-2-yl) amino) butyric acid hapten was designed and synthesized. This maximized the exposure of the antigen-determinant isopropyl of prometryn to the immune system in animals to induce the production of anticipated highly specific anti-prometryn antibodies. The hapten has a molecular weight of 285.37 Da. The compound was confirmed by nuclear magnetic resonance hydrogen spectroscopy (1H NMR), nuclear magnetic resonance carbon spectroscopy (13C NMR), and high-resolution mass spectrometry (HRMS). By using the active ester approach, immunogens and coated antigens were created. Bovine serum albumin (BSA) was used as an immunogen, along with the successfully produced hapten, to immunize mice. The IC50 value of mouse monoclonal anti-prometryn antibody (mAb) 7D4 (the quantity of analyte that generated 50% prometryn inhibition) was 3.9 ng\/mL. The anti-prometryn mAb was of the IgG1 subclass. The IC20 (80% binding level (B\/B0) of prometryn)-IC80 (20% binding level (B\/B0) of prometryn) range of the anti-prometryn monoclonal antibody standard curve working range was 0.9\u201318.1 ng\/mL. The prepared mAb has good characteristics because it can specifically recognize prometryn, and the cross-reaction rates for ametryn, desmetryn, and terbumeton were 34.77%, 18.09%, and 7.64%, respectively. The cross-reaction rate with the other seven triazines was less than 1%. The hapten structure proposed can serve as an additional tool for modulating selectivity in detecting triazines.<\/jats:p>","DOI":"10.3390\/bios13010022","type":"journal-article","created":{"date-parts":[[2022,12,27]],"date-time":"2022-12-27T03:03:31Z","timestamp":1672110211000},"page":"22","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Obtaining a Monoclonal Antibody against a Novel Prometryn-Like Hapten and Characterization of Its Selectivity for Triazine Herbicides"],"prefix":"10.3390","volume":"13","author":[{"given":"Lingyuan","family":"Xu","sequence":"first","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6596-7907","authenticated-orcid":false,"given":"A. M.","family":"Abd El-Aty","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China"},{"name":"Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt"},{"name":"Department of Medical Pharmacology, Medical Faculty, Ataturk University, 25240 Erzurum, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingmei","family":"Lei","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuyuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Yantai University, Yantai 264005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyan","family":"Cui","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7055-4808","authenticated-orcid":false,"given":"Yongxin","family":"She","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fen","family":"Jin","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9102-8572","authenticated-orcid":false,"given":"Maojun","family":"Jin","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1408-8317","authenticated-orcid":false,"given":"Bruce D.","family":"Hammock","sequence":"additional","affiliation":[{"name":"Department of Entomology & Nematology and the UC Davis Comprehensive Cancer Center, University of California, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,25]]},"reference":[{"key":"ref_1","unstructured":"LeBaron, H.M., McFarland, J.E., and Burnside, O. 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