{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:06:53Z","timestamp":1790788013558,"version":"4.1.0"},"reference-count":149,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T00:00:00Z","timestamp":1672704000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea (NRF)","doi-asserted-by":"publisher","award":["2022R1A2C4001652"],"award-info":[{"award-number":["2022R1A2C4001652"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea (NRF)","doi-asserted-by":"publisher","award":["MOST111-2221-E-182-006-MY3"],"award-info":[{"award-number":["MOST111-2221-E-182-006-MY3"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Science and Technology Council, Taiwan","award":["2022R1A2C4001652"],"award-info":[{"award-number":["2022R1A2C4001652"]}]},{"name":"National Science and Technology Council, Taiwan","award":["MOST111-2221-E-182-006-MY3"],"award-info":[{"award-number":["MOST111-2221-E-182-006-MY3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Electrical impedance biosensors are powerful and continuously being developed for various biological sensing applications. In this line, the sensitivity of impedance biosensors embedded with microfluidic technologies, such as sheath flow focusing, dielectrophoretic focusing, and interdigitated electrode arrays, can still be greatly improved. In particular, reagent consumption reduction and analysis time-shortening features can highly increase the analytical capabilities of such biosensors. Moreover, the reliability and efficiency of analyses are benefited by microfluidics-enabled automation. Through the use of mature microfluidic technology, complicated biological processes can be shrunk and integrated into a single microfluidic system (e.g., lab-on-a-chip or micro-total analysis systems). By incorporating electrical impedance biosensors, hand-held and bench-top microfluidic systems can be easily developed and operated by personnel without professional training. Furthermore, the impedance spectrum provides broad information regarding cell size, membrane capacitance, cytoplasmic conductivity, and cytoplasmic permittivity without the need for fluorescent labeling, magnetic modifications, or other cellular treatments. In this review article, a comprehensive summary of microfluidics-based impedance biosensors is presented. The structure of this article is based on the different substrate material categorizations. Moreover, the development trend of microfluidics-based impedance biosensors is discussed, along with difficulties and challenges that may be encountered in the future.<\/jats:p>","DOI":"10.3390\/bios13010083","type":"journal-article","created":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T04:08:07Z","timestamp":1672805287000},"page":"83","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["A Review on Microfluidics-Based Impedance Biosensors"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3238-5756","authenticated-orcid":false,"given":"Yu-Shih","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, Chang Gung University, Taoyuan 33302, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Hao","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Chang Gung University, Taoyuan 33302, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping-Ching","family":"Pai","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8898-044X","authenticated-orcid":false,"given":"Jungmok","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Chang Gung University, Taoyuan 33302, Taiwan"},{"name":"Department of Electrical & Electronic Engineering, Yonsei University, Seoul 120-749, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5356-2221","authenticated-orcid":false,"given":"Kin Fong","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Chang Gung University, Taoyuan 33302, Taiwan"},{"name":"Department of Radiation Oncology, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan"},{"name":"Department of Electrical & Electronic Engineering, Yonsei University, Seoul 120-749, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kim, S., Song, H., Ahn, H., Kim, T., Jung, J., Cho, S.K., Shin, D.M., Choi, J.R., Hwang, Y.H., and Kim, K. 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