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Rapid and accurate diagnosis methods are required to preclude the spread of diseases. In this paper, an ultrafast polymerase chain reaction (PCR) equipment is designed to detect virus. The equipment consists of a silicon-based PCR chip, a thermocycling module, an optical detection module, and a control module. Silicon-based chip, with its thermal and fluid design, is used to improve detection efficiency. A thermoelectric cooler (TEC), together with a computer-controlled proportional\u2013integral\u2013derivative (PID) controller, is applied to accelerate the thermal cycle. A maximum of four samples can be tested simultaneously on the chip. Two kinds of fluorescent molecules can be detected by optical detection module. The equipment can detect viruses with 40 PCR amplification cycles in 5 min. The equipment is portable, easily operated, and low equipment cost, which shows great potential in epidemic prevention.<\/jats:p>","DOI":"10.3390\/bios13020234","type":"journal-article","created":{"date-parts":[[2023,2,8]],"date-time":"2023-02-08T02:04:16Z","timestamp":1675821856000},"page":"234","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Ultrafast Nucleic Acid Detection Equipment with Silicon-Based Microfluidic Chip"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8877-426X","authenticated-orcid":false,"given":"Jiali","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Microelectronics, Shanghai University, Shanghai 201800, China"},{"name":"Shanghai Industrial Technology Research Institute (SITRI), Shanghai 201800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6213-2733","authenticated-orcid":false,"given":"Zhuo","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Microelectronics, Shanghai University, Shanghai 201800, China"},{"name":"Shanghai Industrial Technology Research Institute (SITRI), Shanghai 201800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liying","family":"Liu","sequence":"additional","affiliation":[{"name":"Shanghai Si-Gene Biotechnology Co., Ltd., Shanghai 201800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tinglu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Industrial Technology Research Institute (SITRI), Shanghai 201800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilei","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Microelectronics, Shanghai University, Shanghai 201800, China"},{"name":"Shanghai Industrial Technology Research Institute (SITRI), Shanghai 201800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunrui","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Microelectronics, Shanghai University, Shanghai 201800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hu","family":"Chen","sequence":"additional","affiliation":[{"name":"Shanghai Si-Gene Biotechnology Co., Ltd., Shanghai 201800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruihua","family":"Ding","sequence":"additional","affiliation":[{"name":"Shanghai Industrial Technology Research Institute (SITRI), Shanghai 201800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Microelectronics, Shanghai University, Shanghai 201800, China"},{"name":"Shanghai Industrial Technology Research Institute (SITRI), Shanghai 201800, China"},{"name":"Shanghai Si-Gene Biotechnology Co., Ltd., Shanghai 201800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9988-930X","authenticated-orcid":false,"given":"Chang","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Microelectronics, Shanghai University, Shanghai 201800, China"},{"name":"Shanghai Industrial Technology Research Institute (SITRI), Shanghai 201800, China"},{"name":"Shanghai Si-Gene Biotechnology Co., Ltd., Shanghai 201800, China"},{"name":"State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China"},{"name":"Shanghai Academy of Experimental Medicine, Shanghai 200052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,7]]},"reference":[{"key":"ref_1","unstructured":"WHO (2022, October 14). 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