{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T03:53:10Z","timestamp":1782186790762,"version":"3.54.5"},"reference-count":37,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T00:00:00Z","timestamp":1675728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32272423"],"award-info":[{"award-number":["32272423"]}]},{"name":"National Natural Science Foundation of China","award":["P42 ES004699"],"award-info":[{"award-number":["P42 ES004699"]}]},{"name":"National Natural Science Foundation of China","award":["No. Y2021PT05"],"award-info":[{"award-number":["No. Y2021PT05"]}]},{"name":"National Natural Science Foundation of China","award":["No. R35ES030443"],"award-info":[{"award-number":["No. R35ES030443"]}]},{"name":"NIEHS Superfund Research Program","award":["32272423"],"award-info":[{"award-number":["32272423"]}]},{"name":"NIEHS Superfund Research Program","award":["P42 ES004699"],"award-info":[{"award-number":["P42 ES004699"]}]},{"name":"NIEHS Superfund Research Program","award":["No. Y2021PT05"],"award-info":[{"award-number":["No. Y2021PT05"]}]},{"name":"NIEHS Superfund Research Program","award":["No. R35ES030443"],"award-info":[{"award-number":["No. R35ES030443"]}]},{"name":"Central Public Interest Scientific Institution Basal Research Fund for the Chinese Academy of Agricultural Sciences","award":["32272423"],"award-info":[{"award-number":["32272423"]}]},{"name":"Central Public Interest Scientific Institution Basal Research Fund for the Chinese Academy of Agricultural Sciences","award":["P42 ES004699"],"award-info":[{"award-number":["P42 ES004699"]}]},{"name":"Central Public Interest Scientific Institution Basal Research Fund for the Chinese Academy of Agricultural Sciences","award":["No. Y2021PT05"],"award-info":[{"award-number":["No. Y2021PT05"]}]},{"name":"Central Public Interest Scientific Institution Basal Research Fund for the Chinese Academy of Agricultural Sciences","award":["No. R35ES030443"],"award-info":[{"award-number":["No. R35ES030443"]}]},{"name":"NIEHS RIVER AWARD","award":["32272423"],"award-info":[{"award-number":["32272423"]}]},{"name":"NIEHS RIVER AWARD","award":["P42 ES004699"],"award-info":[{"award-number":["P42 ES004699"]}]},{"name":"NIEHS RIVER AWARD","award":["No. Y2021PT05"],"award-info":[{"award-number":["No. Y2021PT05"]}]},{"name":"NIEHS RIVER AWARD","award":["No. R35ES030443"],"award-info":[{"award-number":["No. R35ES030443"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>In this study, a monoclonal antibody (mAb) specific to forchlorfenuron (CPPU) with high sensitivity and specificity was produced and designated (9G9). To detect CPPU in cucumber samples, an indirect enzyme-linked immunosorbent assay (ic-ELISA) and a colloidal gold nanobead immunochromatographic test strip (CGN-ICTS) were established using 9G9. The half-maximal inhibitory concentration (IC50) and the LOD for the developed ic-ELISA were determined to be 0.19 ng\/mL and 0.04 ng\/mL in the sample dilution buffer, respectively. The results indicate that the sensitivity of the antibodies prepared in this study (9G9 mAb) was higher than those reported in the previous literature. On the other hand, in order to achieve rapid and accurate detection of CPPU, CGN-ICTS is indispensable. The IC50 and the LOD for the CGN-ICTS were determined to be 27 ng\/mL and 6.1 ng\/mL. The average recoveries of the CGN-ICTS ranged from 68 to 82%. The CGN-ICTS and ic-ELISA quantitative results were all confirmed by liquid chromatography\u2014tandem mass spectrometry (LC-MS\/MS) with 84\u201392% recoveries, which indicated the methods developed herein are appropriate for detecting CPPU in cucumber. The CGN-ICTS method is capable of both qualitative and semiquantitative analysis of CPPU, which makes it a suitable alternative complex instrument method for on-site detection of CPPU in cucumber samples since it does not require specialized equipment.<\/jats:p>","DOI":"10.3390\/bios13020239","type":"journal-article","created":{"date-parts":[[2023,2,8]],"date-time":"2023-02-08T02:29:08Z","timestamp":1675823348000},"page":"239","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Production of a Monoclonal Antibody for the Detection of Forchlorfenuron: Application in an Indirect Enzyme-Linked Immunosorbent Assay and Immunochromatographic Strip"],"prefix":"10.3390","volume":"13","author":[{"given":"Xingmei","family":"Lei","sequence":"first","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6596-7907","authenticated-orcid":false,"given":"A. M.","family":"Abd El-Aty","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt"},{"name":"Department of Medical Pharmacology, Medical Faculty, Ataturk University, Erzurum 25240, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyuan","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Li","sequence":"additional","affiliation":[{"name":"Jinhua Miaozhidizhi Agricultural Technology Co., Ltd., Jinhua 321000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Gao","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7055-4808","authenticated-orcid":false,"given":"Yongxin","family":"She","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fen","family":"Jin","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lufei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9102-8572","authenticated-orcid":false,"given":"Maojun","family":"Jin","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"},{"name":"Research Center of Quality Standards for Agro-Products, Ministry of Agriculture and Rural Affairs, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1408-8317","authenticated-orcid":false,"given":"Bruce D.","family":"Hammock","sequence":"additional","affiliation":[{"name":"Department of Entomology & Nematology and the UC Davis Comprehensive Cancer Center, University of California, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,7]]},"reference":[{"key":"ref_1","first-page":"4874","article-title":"Peer Review of the Pesticide Risk Assessment of the Active Substance Forchlorfenuron","volume":"15","author":"Arena","year":"2017","journal-title":"EFSA J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"112412","DOI":"10.1016\/j.foodres.2022.112412","article-title":"Key metabolites and mechanistic insights in forchlorfenuron controlling kiwifruit development","volume":"164","author":"Bi","year":"2023","journal-title":"Food Res. Int."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.reprotox.2020.09.009","article-title":"Effects of prepubertal exposure to forchlorfenuron through prenatal and postnatal gavage administration in developing Sprague-Dawley rats","volume":"98","author":"Zhu","year":"2020","journal-title":"Reprod. Toxicol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.chemosphere.2019.06.027","article-title":"Cardiotoxicity of forchlorfenuron (CPPU) in zebrafish (Danio rerio) and H9c2 cardiomyocytes","volume":"235","author":"Gong","year":"2019","journal-title":"Chemosphere"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3059","DOI":"10.1021\/acs.jafc.5b01100","article-title":"Identification, Synthesis, and Safety Assessment of Forchlorfenuron (1-(2-Chloro-4-pyridyl)-3-phenylurea) and Its Metabolites in Kiwifruits","volume":"63","author":"Zhang","year":"2015","journal-title":"J. Agric. Food Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"10207","DOI":"10.1021\/acs.jafc.9b03855","article-title":"180 Day Repeated-Dose Toxicity Study on Forchlorfenuron in Sprague\u2013Dawley Rats and Its Effects on the Production of Steroid Hormones","volume":"67","author":"Bu","year":"2019","journal-title":"J. Agric. Food Chem."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Ping, L., Xu, B., Zhou, Q., Hong, Y., Sun, Q., Wang, J., and Zhu, D. (2021). Comparative Pharmacokinetic Study of Forchlorfenuron in Adult and Juvenile Rats. Molecules, 26.","DOI":"10.3390\/molecules26144276"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"461192","DOI":"10.1016\/j.chroma.2020.461192","article-title":"Imidazolium ionic-liquid-modified phenolic resin for solid-phase extraction of thidiazuron and forchlorfenuron from cucumbers","volume":"1623","author":"Li","year":"2020","journal-title":"J. Chromatogr. A"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2398","DOI":"10.1016\/j.foodchem.2012.04.034","article-title":"Finding of Pesticides in Fashionable Fruit Juices by Lc-Ms\/Ms and Gc-Ms\/Ms","volume":"134","author":"Tran","year":"2012","journal-title":"Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1080\/19440049.2021.1876250","article-title":"Rapid analysis of forchlorfenuron in fruits using molecular complex-based dispersive liquid\u2013liquid microextraction","volume":"38","author":"Ren","year":"2021","journal-title":"Food Addit. Contam. Part A"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"6383","DOI":"10.1021\/jf201618q","article-title":"Collaborative Validation of the QuEChERS Procedure for the Determination of Pesticides in Food by LC\u2013MS\/MS","volume":"59","author":"Sack","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1007\/s10661-022-10647-y","article-title":"Validation of simple and efficient analytical methodology for estimation of forchlorfenuron residues","volume":"195","author":"Sharma","year":"2022","journal-title":"Environ. Monit. Assess."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1002\/jssc.201900771","article-title":"Simultaneous determination of multiclass plant growth regulators in fruits using the quick, easy, cheap, effective, rugged, and safe method and ultra-high performance liquid chromatography-tandem mass spectrometry","volume":"43","author":"Xu","year":"2019","journal-title":"J. Sep. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2340","DOI":"10.1002\/jssc.201600200","article-title":"Analysis of Forchlorfenuron and Thidiazuron in Fruits and Vegetables by Surface-Enhanced Raman Spectroscopy after Selective Solid-Phase Extraction with Modified Beta-Cyclodextrin","volume":"39","author":"Chen","year":"2016","journal-title":"J. Sep. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"5961","DOI":"10.1007\/s00216-018-1213-5","article-title":"Development of immunosorbents for the analysis of forchlorfenuron in fruit juices by ion mobility spectrometry","volume":"410","author":"Armenta","year":"2018","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Liu, X., Xie, B., Cheng, Y., Luo, L., Liang, Y., and Xiao, Z. (2022). A Sensitive Monoclonal-Antibody-Based ELISA for Forchlorfenuron Residue Analysis in Food Samples. Biosensors, 12.","DOI":"10.3390\/bios12020078"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2019","DOI":"10.1007\/s00216-012-5991-x","article-title":"Development and validation of a direct competitive monoclonal antibody-based immunoassay for the sensitive and selective analysis of the phytoregulator forchlorfenuron","volume":"403","author":"Mercader","year":"2012","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"8502","DOI":"10.1021\/jf101660z","article-title":"Hapten Synthesis and Polyclonal Antibody-Based Immunoassay Development for the Analysis of Forchlorfenuron in Kiwifruit","volume":"58","author":"Mercader","year":"2010","journal-title":"J. Agric. Food Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.bios.2012.11.001","article-title":"Development of an immunochromatographic assay based on carbon nanoparticles for the determination of the phytoregulator forchlorfenuron","volume":"42","author":"Wichers","year":"2013","journal-title":"Biosens. Bioelectron."},{"key":"ref_20","unstructured":"Xiao, S.M. (2017). The Establishment of Gold Nanoparticles-Colorimetric and Immunoassay Analysis Method on Detection of Pesticide Residues in Environment and Food. [Master\u2019s Thesis, Nanchang University]."},{"key":"ref_21","first-page":"5584","article-title":"Preparation of Anti-CPPU Monoclonal Antibody and Development of an Indirect Competitive ELISA Method","volume":"55","author":"Lu","year":"2016","journal-title":"Hubei Agric. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1021\/acsomega.9b01841","article-title":"Colloidal Gold Immunochromatographic Strip Assay for the Detection of Azaperone in Pork and Pork Liver","volume":"5","author":"Lin","year":"2020","journal-title":"ACS Omega"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2407","DOI":"10.1016\/j.psj.2019.11.066","article-title":"Development and application of a colloidal gold test strip for the rapid detection of the infectious laryngotracheitis virus","volume":"99","author":"Yu","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1080\/09540105.2019.1708272","article-title":"Simultaneous and rapid detection of carbofuran and 3-hydroxy-carbofuran in water samples and pesticide preparations using lateral-flow immunochromatographic assay","volume":"31","author":"Lan","year":"2020","journal-title":"Food Agric. Immunol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1080\/09540105.2020.1733496","article-title":"Development and validation of an immunochromatography test strip for rapid detection of pyrimethanil residues","volume":"31","author":"Xie","year":"2020","journal-title":"Food Agric. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"131284","DOI":"10.1016\/j.foodchem.2021.131284","article-title":"Preparing monoclonal antibodies and developing immunochromatographic assay strips for the determination of propamocarb levels","volume":"370","author":"Liu","year":"2022","journal-title":"Food Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1080\/09540105.2015.1018875","article-title":"Development of a colloidal gold-based strip test for the detection of chlorothalonil residues in cucumber","volume":"26","author":"Yang","year":"2015","journal-title":"Food Agric. Immunol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"90","DOI":"10.2144\/btn-2019-0054","article-title":"Hybridoma technology: The preferred method for monoclonal antibody generation for in vivo applications","volume":"67","author":"Zaroff","year":"2019","journal-title":"Biotechniques"},{"key":"ref_29","first-page":"54832","article-title":"Generation of Murine Monoclonal Antibodies by Hybridoma Technology","volume":"119","author":"Holzlohner","year":"2017","journal-title":"J. Vis. Exp."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Xu, L., El-Aty, A.A., Shim, J.-H., Eun, J.-B., Lei, X., Zhao, J., Zhang, X., Cui, X., She, Y., and Jin, F. (2022). Design and Characterization of a Novel Hapten and Preparation of Monoclonal Antibody for Detecting Atrazine. Foods, 11.","DOI":"10.3390\/foods11121726"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/j.tibtech.2007.07.002","article-title":"Selection methods for high-producing mammalian cell lines","volume":"25","author":"Browne","year":"2007","journal-title":"Trends Biotechnol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"126055","DOI":"10.1016\/j.foodchem.2019.126055","article-title":"Ultrasensitive quantitation of imidacloprid in vegetables by colloidal gold and time-resolved fluorescent nanobead traced lateral flow immunoassays","volume":"311","author":"Tan","year":"2019","journal-title":"Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"129710","DOI":"10.1016\/j.foodchem.2021.129710","article-title":"Lateral flow immunoassay for the simultaneous detection of fipronil and its metabolites in food samples","volume":"356","author":"Yao","year":"2021","journal-title":"Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"16680","DOI":"10.1038\/s41598-018-35080-5","article-title":"A Lateral Flow Immunochromato-graphic Strip Test for Rapid Detection of Oseltamivir Phosphate in Egg and Chicken Meat","volume":"8","author":"Yang","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1935","DOI":"10.1039\/C9TB02839K","article-title":"Production of a monoclonal antibody for the detection of vitamin B1 and its use in an indirect enzyme-linked immunosorbent assay and immunochromatographic strip","volume":"8","author":"Zeng","year":"2020","journal-title":"J. Mater. Chem. B"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"23","DOI":"10.2174\/0929867053363540","article-title":"Bioisosterism: A Useful Strategy for Molecular Modification and Drug Design","volume":"12","author":"Lima","year":"2005","journal-title":"Curr. Med. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"4863","DOI":"10.1039\/c1ob05190c","article-title":"Forchlorfenuron-mimicking haptens: From immunogen design to antibody characterization by hierarchical clustering analysis","volume":"9","author":"Mercader","year":"2011","journal-title":"Org. Biomol. Chem."}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/13\/2\/239\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:27:17Z","timestamp":1760120837000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/13\/2\/239"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,7]]},"references-count":37,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["bios13020239"],"URL":"https:\/\/doi.org\/10.3390\/bios13020239","relation":{},"ISSN":["2079-6374"],"issn-type":[{"value":"2079-6374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,7]]}}}