{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T11:04:58Z","timestamp":1771067098687,"version":"3.50.1"},"reference-count":34,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,3,6]],"date-time":"2023-03-06T00:00:00Z","timestamp":1678060800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Jiangsu Maternal and Child Health Research Project","award":["F202144"],"award-info":[{"award-number":["F202144"]}]},{"name":"Jiangsu Maternal and Child Health Research Project","award":["SH2022098"],"award-info":[{"award-number":["SH2022098"]}]},{"name":"Jiangsu Maternal and Child Health Research Project","award":["XTCX2026"],"award-info":[{"award-number":["XTCX2026"]}]},{"name":"Jiangsu Maternal and Child Health Research Project","award":["G2022014094L"],"award-info":[{"award-number":["G2022014094L"]}]},{"name":"Jiangsu Maternal and Child Health Research Project","award":["DL2022014006L"],"award-info":[{"award-number":["DL2022014006L"]}]},{"name":"Zhenjiang Science and Technology Innovation Fund","award":["F202144"],"award-info":[{"award-number":["F202144"]}]},{"name":"Zhenjiang Science and Technology Innovation Fund","award":["SH2022098"],"award-info":[{"award-number":["SH2022098"]}]},{"name":"Zhenjiang Science and Technology Innovation Fund","award":["XTCX2026"],"award-info":[{"award-number":["XTCX2026"]}]},{"name":"Zhenjiang Science and Technology Innovation Fund","award":["G2022014094L"],"award-info":[{"award-number":["G2022014094L"]}]},{"name":"Zhenjiang Science and Technology Innovation Fund","award":["DL2022014006L"],"award-info":[{"award-number":["DL2022014006L"]}]},{"name":"Jiangsu Province and Education Ministry Co-sponsored Synergistic Innovation Center of Modern Agricultural Equipment of China","award":["F202144"],"award-info":[{"award-number":["F202144"]}]},{"name":"Jiangsu Province and Education Ministry Co-sponsored Synergistic Innovation Center of Modern Agricultural Equipment of China","award":["SH2022098"],"award-info":[{"award-number":["SH2022098"]}]},{"name":"Jiangsu Province and Education Ministry Co-sponsored Synergistic Innovation Center of Modern Agricultural Equipment of China","award":["XTCX2026"],"award-info":[{"award-number":["XTCX2026"]}]},{"name":"Jiangsu Province and Education Ministry Co-sponsored Synergistic Innovation Center of Modern Agricultural Equipment of China","award":["G2022014094L"],"award-info":[{"award-number":["G2022014094L"]}]},{"name":"Jiangsu Province and Education Ministry Co-sponsored Synergistic Innovation Center of Modern Agricultural Equipment of China","award":["DL2022014006L"],"award-info":[{"award-number":["DL2022014006L"]}]},{"name":"National Foreign Experts Program Project of China","award":["F202144"],"award-info":[{"award-number":["F202144"]}]},{"name":"National Foreign Experts Program Project of China","award":["SH2022098"],"award-info":[{"award-number":["SH2022098"]}]},{"name":"National Foreign Experts Program Project of China","award":["XTCX2026"],"award-info":[{"award-number":["XTCX2026"]}]},{"name":"National Foreign Experts Program Project of China","award":["G2022014094L"],"award-info":[{"award-number":["G2022014094L"]}]},{"name":"National Foreign Experts Program Project of China","award":["DL2022014006L"],"award-info":[{"award-number":["DL2022014006L"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>As a biomarker, alpha-fetoprotein (AFP) is valuable for detecting some tumors in men, non-pregnant women, and children. However, the detection sensitivity in some methods needs to be improved. Therefore, developing a simple, reliable, and sensitive detection method for AFP is important for non-malignant diseases. An aptamer binding was developed based on aggregation-induced emission luminogen (AIEgen) nanosphere labeled with Fe3O4@MPTMS@AuNPs. AFP was detected with a sandwich structure of AuNPs magnetic composite particles. An aggregation-induced emission (AIE) molecule and polystyrene (PS) nanosphere complex were assembled, enhancing the fluorescence and improving the sensitivity of detection. The limit of detection (LOD) was at a given level of 1.429 pg\/mL, which can best be achieved in serum samples. Finally, the results obtained showed the complex to be promising in practical applications.<\/jats:p>","DOI":"10.3390\/bios13030351","type":"journal-article","created":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T02:07:38Z","timestamp":1678154858000},"page":"351","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Detection of Alpha Fetoprotein Based on AIEgen Nanosphere Labeled Aptamer Combined with Sandwich Structure of Magnetic Gold Nanocomposites"],"prefix":"10.3390","volume":"13","author":[{"given":"Lei","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Kidney Transplantation, The Second Xiangya Hospital of Central South University, Changsha 410011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huixing","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Jiangsu University, Zhenjiang 212013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Husseini","family":"Sulemana","sequence":"additional","affiliation":[{"name":"School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Xie","sequence":"additional","affiliation":[{"name":"The Fourth Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Jiangsu University, Zhenjiang 212013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1049\/mnl.2019.0267","article-title":"Ultrasensitive immunosensor for AFP detection based on Cu2O to generate electrical signals","volume":"15","author":"Zhao","year":"2020","journal-title":"Micro Nano Lett."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"101103","DOI":"10.1016\/j.surfin.2021.101103","article-title":"Conjugated polymer sensitized hyperbranched titanium dioxide based photoelectrochemical biosensor for detecting AFP in serum","volume":"24","author":"Mo","year":"2021","journal-title":"Surf. 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