{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T12:59:52Z","timestamp":1778590792430,"version":"3.51.4"},"reference-count":32,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,5,13]],"date-time":"2023-05-13T00:00:00Z","timestamp":1683936000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Central Plains Science and Technology Innovation Leading Talent Project","award":["234200510012"],"award-info":[{"award-number":["234200510012"]}]},{"name":"Central Plains Science and Technology Innovation Leading Talent Project","award":["2023TD18"],"award-info":[{"award-number":["2023TD18"]}]},{"name":"Central Plains Science and Technology Innovation Leading Talent Project","award":["2023ZC073"],"award-info":[{"award-number":["2023ZC073"]}]},{"name":"Scientific-Technological Innovation of Henan Academy of Agricultural Sciences","award":["234200510012"],"award-info":[{"award-number":["234200510012"]}]},{"name":"Scientific-Technological Innovation of Henan Academy of Agricultural Sciences","award":["2023TD18"],"award-info":[{"award-number":["2023TD18"]}]},{"name":"Scientific-Technological Innovation of Henan Academy of Agricultural Sciences","award":["2023ZC073"],"award-info":[{"award-number":["2023ZC073"]}]},{"name":"Independence Innovation Projects of Henan Academy of Agricultural Sciences","award":["234200510012"],"award-info":[{"award-number":["234200510012"]}]},{"name":"Independence Innovation Projects of Henan Academy of Agricultural Sciences","award":["2023TD18"],"award-info":[{"award-number":["2023TD18"]}]},{"name":"Independence Innovation Projects of Henan Academy of Agricultural Sciences","award":["2023ZC073"],"award-info":[{"award-number":["2023ZC073"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Pyridaben, a broad-spectrum pyridazinone acaricide that is widely used in agricultural production, can induce neurotoxicity and reproductive abnormalities, and is highly toxic to aquatic organisms. In this study, a pyridaben hapten was synthesized and used to prepare monoclonal antibodies (mAbs), among which 6E3G8D7 showed the highest sensitivity in indirect competitive enzyme-linked immunosorbent assay, with a 50% inhibitory concentration (IC50) of 3.49 ng mL\u22121. The mAb, 6E3G8D7, was further applied to a gold nanoparticle-based colorimetric lateral flow immunoassay (CLFIA) for pyridaben detection, according to the signal intensity ratio of the test line to the control line, which showed a visual limit of detection of 5 ng mL\u22121. The CLFIA also showed high specificity and achieved excellent accuracy in different matrices. In addition, the amounts of pyridaben in blind samples detected by the CLFIA, were consistent with high-performance liquid chromatography. Therefore, the developed CLFIA is considered a promising, reliable, and portable method for pyridaben on-site detection in agro-products and environmental samples.<\/jats:p>","DOI":"10.3390\/bios13050545","type":"journal-article","created":{"date-parts":[[2023,5,15]],"date-time":"2023-05-15T08:33:01Z","timestamp":1684139581000},"page":"545","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Monoclonal Antibody-Based Colorimetric Lateral Flow Immunoassay for the Detection of Pyridaben in the Environment"],"prefix":"10.3390","volume":"13","author":[{"given":"He","family":"Chen","sequence":"first","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China"},{"name":"Key Laboratory of Grain Quality and Safety and Testing Henan Province, Zhengzhou 450002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Henan University, Kaifeng 475004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanran","family":"Ji","sequence":"additional","affiliation":[{"name":"College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zekun","family":"Sha","sequence":"additional","affiliation":[{"name":"College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"An","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China"},{"name":"Key Laboratory of Grain Quality and Safety and Testing Henan Province, Zhengzhou 450002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China"},{"name":"Key Laboratory of Grain Quality and Safety and Testing Henan Province, Zhengzhou 450002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Di","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China"},{"name":"Key Laboratory of Grain Quality and Safety and Testing Henan Province, Zhengzhou 450002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xujin","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China"},{"name":"Key Laboratory of Grain Quality and Safety and Testing Henan Province, Zhengzhou 450002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0557-1892","authenticated-orcid":false,"given":"Xiude","family":"Hua","sequence":"additional","affiliation":[{"name":"College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"104733","DOI":"10.1016\/j.pestbp.2020.104733","article-title":"Pyridaben leads to inhibition of cell growth and induction of cell death through intracellular mechanisms in early pregnancy","volume":"171","author":"Bae","year":"2021","journal-title":"Pestic. 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