{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:13:40Z","timestamp":1780445620885,"version":"3.54.1"},"reference-count":26,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T00:00:00Z","timestamp":1684368000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Defense Industrial Technology Development Program","award":["JCKY2021602B009"],"award-info":[{"award-number":["JCKY2021602B009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>PCR is indispensable in basic science and biotechnology for in-orbit life science research. However, manpower and resources are limited in space. To address the constraints of in-orbit PCR, we proposed an oscillatory-flow PCR technique based on biaxial centrifugation. Oscillatory-flow PCR remarkably reduces the power requirements of the PCR process and has a relatively high ramp rate. A microfluidic chip that could perform dispensing, volume correction, and oscillatory-flow PCR of four samples simultaneously using biaxial centrifugation was designed. An automatic biaxial centrifugation device was designed and assembled to validate the biaxial centrifugation oscillatory-flow PCR. Simulation analysis and experimental tests indicated that the device could perform fully automated PCR amplification of four samples in one hour, with a ramp rate of 4.4 \u2218C\/s and average power consumption of less than 30 W. The PCR results were consistent with those obtained using conventional PCR equipment. Air bubbles generated during amplification were removed by oscillation. The chip and device realized a low-power, miniaturized, and fast PCR method under microgravity conditions, indicating good space application prospects and potential for higher throughput and extension to qPCR.<\/jats:p>","DOI":"10.3390\/bios13050555","type":"journal-article","created":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T07:35:50Z","timestamp":1684395350000},"page":"555","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Oscillatory-Flow PCR Microfluidic Chip Driven by Low Speed Biaxial Centrifugation"],"prefix":"10.3390","volume":"13","author":[{"given":"Yunlong","family":"Fan","sequence":"first","affiliation":[{"name":"Key Laboratory of Convergence Medical Engineering System and Healthcare Technology, the Ministry of Industry and Information Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China 2 Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Sciences, Beijing Institute of Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China"},{"name":"Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Sciences, Beijing Institute of Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongji","family":"Dai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Convergence Medical Engineering System and Healthcare Technology, the Ministry of Industry and Information Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China 2 Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Sciences, Beijing Institute of Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China"},{"name":"Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Sciences, Beijing Institute of Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyu","family":"Lu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Convergence Medical Engineering System and Healthcare Technology, the Ministry of Industry and Information Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China 2 Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Sciences, Beijing Institute of Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China"},{"name":"Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Sciences, Beijing Institute of Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, 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Technology, the Ministry of Industry and Information Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China 2 Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Sciences, Beijing Institute of Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China"},{"name":"Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Sciences, Beijing Institute of Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhua","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Convergence Medical Engineering System and Healthcare Technology, the Ministry of Industry and Information Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China 2 Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Sciences, Beijing Institute of Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China"},{"name":"Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Sciences, Beijing Institute of Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Convergence Medical Engineering System and Healthcare Technology, the Ministry of Industry and Information Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China 2 Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Sciences, Beijing Institute of Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China"},{"name":"Beijing Key Laboratory for Separation and Analysis in 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/14737159.2020.1699061","article-title":"Diagnostics in space: Will zero gravity add weight to new advances?","volume":"20","author":"Wong","year":"2020","journal-title":"Expert Rev. 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