{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T11:16:46Z","timestamp":1784027806740,"version":"3.55.0"},"reference-count":110,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T00:00:00Z","timestamp":1695859200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Auxotrophic primates like human beings rely on exogenous dietary vitamin B9 supplementation to meet their metabolic demands. Folates play a crucial role in nucleotide synthesis and DNA methylation. Maternal folate deficiency causes several pregnancy-related complications, perinatal defects, and early childhood cognitive impairments. New evidence suggests excess FA is a potential risk factor resulting in unfavourable genomic and epigenomic alterations. Thus, it is essential to revisit the need to consistently monitor maternal folate levels during pregnancy. Yet, to date, no point-of-care folate-monitoring biosensor is commercially available. Here, we critically appraise the advances in folate biosensors to understand the translational gaps in biosensor design. Further, our review sheds light on the potential role of folate biosensors in strengthening maternal, perinatal, and child healthcare.<\/jats:p>","DOI":"10.3390\/bios13100912","type":"journal-article","created":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T08:24:44Z","timestamp":1695889484000},"page":"912","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Advances in Folic Acid Biosensors and Their Significance in Maternal, Perinatal, and Paediatric Preventive Medicine"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5843-9102","authenticated-orcid":false,"given":"Yogesh","family":"Movendane","sequence":"first","affiliation":[{"name":"Singapore Institute of Manufacturing Technology (SIMTech), Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis #08-04, Singapore 138634, Singapore"},{"name":"Department of Biomedical Engineering, College of Design and Engineering, National University of Singapore, Singapore 117583, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mbozu G.","family":"Sipalo","sequence":"additional","affiliation":[{"name":"London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7712-1060","authenticated-orcid":false,"given":"Leon C. Z.","family":"Chan","sequence":"additional","affiliation":[{"name":"Singapore Institute of Manufacturing Technology (SIMTech), Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis #08-04, Singapore 138634, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Barua, S., Kuizon, S., and Junaid, M.A. (2014). Folic acid supplementation in pregnancy and implications in health and disease. J. Biomed. Sci., 21.","DOI":"10.1186\/s12929-014-0077-z"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.cmet.2016.08.009","article-title":"One-Carbon Metabolism in Health and Disease","volume":"25","author":"Ducker","year":"2017","journal-title":"Cell Metab."},{"key":"ref_3","unstructured":"Agata, S.M. (2018). Laboratory Assessment of Vitamin Status, Elsevier."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/S0079-6603(01)67027-2","article-title":"Molecular and cellular biology of the human reduced folate carrier","volume":"67","author":"Matherly","year":"2001","journal-title":"Prog. Nucleic Acid. Res. Mol. Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e4","DOI":"10.1017\/S1462399409000969","article-title":"Membrane transporters and folate homeostasis: Intestinal absorption and transport into systemic compartments and tissues","volume":"11","author":"Zhao","year":"2009","journal-title":"Expert Rev. Mol. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.ejps.2016.08.045","article-title":"Stability of folic acid under several parameters","volume":"93","author":"Gazzali","year":"2016","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.procbio.2020.01.025","article-title":"Folic acid biosensors: A review","volume":"92","author":"Batra","year":"2020","journal-title":"Process Biochem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"9541","DOI":"10.1038\/s41598-023-36484-8","article-title":"Prenatal folic acid supplement\/dietary folate and cognitive development in 4-year-old offspring from the Japan Environment and Children\u2019s Study","volume":"13","author":"Nishigori","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Nguyen, P.H., DiGirolamo, A.M., Gonzalez-Casanova, I., Pham, H., Hao, W., Nguyen, H., Truong, T.V., Nguyen, S., Harding, K.B., and Reinhart, G.A. (2017). Impact of preconceptional micronutrient supplementation on maternal mental health during pregnancy and postpartum: Results from a randomized controlled trial in Vietnam. BMC Womens Health, 17.","DOI":"10.1186\/s12905-017-0401-3"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1038\/bjc.2015.446","article-title":"Supplemental folic acid in pregnancy and childhood cancer risk","volume":"114","author":"Mortensen","year":"2016","journal-title":"Br. J. Cancer"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"i110","DOI":"10.1093\/ije\/dyq028","article-title":"Folic acid to reduce neonatal mortality from neural tube disorders","volume":"39","author":"Blencowe","year":"2010","journal-title":"Int. J. Epidemiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1007\/s00404-018-4823-4","article-title":"Supplementation of folic acid in pregnancy and the risk of preeclampsia and gestational hypertension: A meta-analysis","volume":"298","author":"Liu","year":"2018","journal-title":"Arch. Gynecol. Obstet."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.ogrm.2019.01.008","article-title":"Folic acid in pregnancy","volume":"29","author":"Argyridis","year":"2019","journal-title":"Obstet. Gynaecol. Reprod. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s40985-018-0079-6","article-title":"Public health failure in the prevention of neural tube defects: Time to abandon the tolerable upper intake level of folate","volume":"39","author":"Wald","year":"2018","journal-title":"Public Health Rev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e1053","DOI":"10.1016\/S2214-109X(22)00213-3","article-title":"Preventing birth defects, saving lives, and promoting health equity: An urgent call to action for universal mandatory food fortification with folic acid","volume":"10","author":"Kancherla","year":"2022","journal-title":"Lancet Glob. Health"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e1389","DOI":"10.1016\/S2214-109X(22)00378-3","article-title":"Mandatory food fortification with folic acid","volume":"10","author":"Smith","year":"2022","journal-title":"Lancet Glob. Health"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1038\/ejcn.2016.194","article-title":"The adverse effects of an excessive folic acid intake","volume":"71","author":"Patel","year":"2017","journal-title":"Eur. J. Clin. Nutr."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1038\/s41562-023-01522-y","article-title":"Changes in preterm birth and stillbirth during COVID-19 lockdowns in 26 countries","volume":"7","author":"Calvert","year":"2023","journal-title":"Nat. Hum. Behav."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Vaccaro, C., Mahmoud, F., Aboulatta, L., Aloud, B., and Eltonsy, S. (2021). The impact of COVID-19 first wave national lockdowns on perinatal outcomes: A rapid review and meta-analysis. BMC Pregnancy Childbirth, 21.","DOI":"10.1186\/s12884-021-04156-y"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1084\/jem.20181965","article-title":"Toward a better understanding of folate metabolism in health and disease","volume":"216","author":"Zheng","year":"2019","journal-title":"J. Exp. Med."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Franco, C.N., Seabrook, L.J., Nguyen, S.T., Leonard, J.T., and Albrecht, L.V. (2022). Simplifying the B Complex: How Vitamins B6 and B9 Modulate One Carbon Metabolism in Cancer and Beyond. Metabolites, 12.","DOI":"10.3390\/metabo12100961"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Virdi, S., and Jadavji, N.M. (2022). The Impact of Maternal Folates on Brain Development and Function after Birth. Metabolites, 12.","DOI":"10.3390\/metabo12090876"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1111\/nyas.13548","article-title":"Estimates of global and regional prevalence of neural tube defects for 2015: A systematic analysis","volume":"1414","author":"Blencowe","year":"2018","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1186\/s12978-022-01509-4","article-title":"Global prevalence of congenital anencephaly: A comprehensive systematic review and meta-analysis","volume":"19","author":"Salari","year":"2022","journal-title":"Reprod. Health"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1097\/GIM.0b013e31821d4188","article-title":"Policy statement on folic acid and neural tube defects","volume":"13","author":"Toriello","year":"2011","journal-title":"Genet. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1007\/s00381-023-05988-z","article-title":"Spina bifida management in low- and middle-income countries\u2014A comprehensive policy approach","volume":"39","author":"Pattisapu","year":"2023","journal-title":"Child\u2019s Nerv. Syst."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1017\/S136898001100334X","article-title":"Effect on risk of anencephaly of gene-nutrient interactions between methylenetetrahydrofolate reductase C677T polymorphism and maternal folate, vitamin B12 and homocysteine profile","volume":"15","year":"2012","journal-title":"Public Health Nutr."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ho, P., Quigley, M.A., Tatwavedi, D., Britto, C., and Kurinczuk, J.J. (2021). Neonatal and infant mortality associated with spina bifida: A systematic review and meta-analysis. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0250098"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1001\/jama.2016.19193","article-title":"Folic acid supplementation for the prevention of neural tube defects an updated evidence report and systematic review for the US preventive services task force","volume":"317","author":"Viswanathan","year":"2017","journal-title":"JAMA\u2014J. Am. Med. Assoc."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e24","DOI":"10.2105\/AJPH.2015.302902","article-title":"Global birth prevalence of spina bifida by folic acid fortification status: A systematic review and meta-analysis","volume":"106","author":"Atta","year":"2016","journal-title":"Am. J. Public Health"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e1041","DOI":"10.1016\/S2214-109X(23)00191-2","article-title":"Neural tube defects as a cause of death among stillbirths, infants, and children younger than 5 years in sub-Saharan Africa and southeast Asia: An analysis of the CHAMPS network","volume":"11","author":"Madrid","year":"2023","journal-title":"Lancet Glob. Health"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1186\/s12937-018-0421-3","article-title":"Periconceptional folic acid supplementation and sex difference in prevention of neural tube defects and their subtypes in China: Results from a large prospective cohort study","volume":"17","author":"Liu","year":"2018","journal-title":"Nutr. J."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Ssentongo, P., Heilbrunn, E.S., Ssentongo, A.E., Ssenyonga, L.V.N., and Lekoubou, A. (2022). Birth prevalence of neural tube defects in eastern Africa: A systematic review and meta-analysis. BMC Neurol., 22.","DOI":"10.1186\/s12883-022-02697-z"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Caffrey, A., McNulty, H., Rollins, M., Prasad, G., Gaur, P., Talcott, J.B., Witton, C., Cassidy, T., Marshall, B., and Dornan, J. (2021). Effects of maternal folic acid supplementation during the second and third trimesters of pregnancy on neurocognitive development in the child: An 11-year follow-up from a randomised controlled trial. BMC Med., 19.","DOI":"10.1186\/s12916-021-01914-9"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1038\/s41440-022-00965-6","article-title":"Hypertensive disorders of pregnancy: Definition, management, and out-of-office blood pressure measurement","volume":"45","author":"Metoki","year":"2022","journal-title":"Hypertens. Res."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Serrano, N.C., Quintero-Lesmes, D.C., Becerra-Bayona, S., Guio, E., Beltran, M., Paez, M.C., Ortiz, R., Saldarriaga, W., Diaz, L.A., and Monterrosa, \u00c1. (2018). Association of pre-eclampsia risk with maternal levels of folate, homocysteine and vitamin B12 in Colombia: A case-control study. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0208137"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"k3478","DOI":"10.1136\/bmj.k3478","article-title":"Effect of high dose folic acid supplementation in pregnancy on pre-eclampsia (FACT): Double blind, phase III, randomised controlled, international, multicentre trial","volume":"362","author":"Wen","year":"2018","journal-title":"BMJ"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"4149","DOI":"10.1007\/s12325-020-01474-z","article-title":"Folic Acid Supplementation in Patients with Elevated Homocysteine Levels","volume":"37","author":"Kaye","year":"2020","journal-title":"Adv. Ther."},{"key":"ref_39","first-page":"2068","article-title":"High-Dose Folic Acid Does Not Prevent Preeclampsia","volume":"320","author":"Slomski","year":"2018","journal-title":"JAMA"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Asres, A.W., Samuel, S., Daga, W.B., Tena, A., Alemu, A., Workie, S.B., Alemayehu, M., and Messel, H. (2023). Association between iron-folic acid supplementation and pregnancy-induced hypertension among pregnant women in public hospitals, Wolaita Sodo, Ethiopia 2021: A case-control study. BMC Public Health, 23.","DOI":"10.1186\/s12889-023-15794-6"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"847136","DOI":"10.3389\/fnut.2022.847136","article-title":"Folic Acid Levels During Pregnancy Regulate Trophoblast Invasive Behavior and the Possible Development of Preeclampsia","volume":"9","author":"Rahat","year":"2022","journal-title":"Front. Nutr."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e77","DOI":"10.1016\/j.wombi.2017.08.128","article-title":"Folic acid supplement use and the risk of gestational hypertension and preeclampsia","volume":"31","author":"Araneta","year":"2018","journal-title":"Women Birth"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Agrawal, S., Fledderjohann, J., Vellakkal, S., and Stuckler, D. (2015). Adequately diversified dietary intake and iron and folic acid supplementation during pregnancy is associated with reduced occurrence of symptoms suggestive of pre-eclampsia or eclampsia in indian women. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0119120"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Kaldygulova, L., Ukybassova, T., Aimagambetova, G., Gaiday, A., and Tussupkaliyev, A. (2023). Biological Role of Folic Acid in Pregnancy and Possible Therapeutic Application for the Prevention of Preeclampsia. Biomedicines, 11.","DOI":"10.3390\/biomedicines11020272"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/s40748-017-0065-x","article-title":"Folic acid level and preterm birth among Sudanese women","volume":"3","author":"Sharif","year":"2017","journal-title":"Matern. Health Neonatol. Perinatol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2181","DOI":"10.1007\/s00394-020-02409-8","article-title":"The association between periconceptional folic acid supplementation and the risk of preterm birth: A population-based retrospective cohort study of 200,000 women in China","volume":"60","author":"Wu","year":"2021","journal-title":"Eur. J. Nutr."},{"key":"ref_47","unstructured":"Gosavi, A., Amin, Z., David Carter, S., Choolani, M., Fee, E., Milad, M., and Jobe, A. (2023). Antenatal corticosteroids in Singapore: A clinical and scientific assessment. Singap. Med. J."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.3389\/fnins.2019.01284","article-title":"Folic Acid and Risk of Preterm Birth: A Meta-Analysis","volume":"13","author":"Li","year":"2019","journal-title":"Front. Neurosci."},{"key":"ref_49","first-page":"1281","article-title":"Maternal postpartum plasma folate status and preterm birth in a high-risk US population","volume":"22","author":"Olapeju","year":"2019","journal-title":"Public Health Nutr."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"e970","DOI":"10.1016\/S2214-109X(22)00180-2","article-title":"The causes of stillbirths in south Asia: Results from a prospective study in India and Pakistan (PURPOSe)","volume":"10","author":"McClure","year":"2022","journal-title":"Lancet Glob. Health"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Aminu, M., Bar-Zeev, S., White, S., Mathai, M., and Van Den Broek, N. (2019). Understanding cause of stillbirth: A prospective observational multi-country study from sub-Saharan Africa. BMC Pregnancy Childbirth, 19.","DOI":"10.1186\/s12884-019-2626-7"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.1080\/14767058.2017.1419177","article-title":"Criteria for assigning cause of death for stillbirths and neonatal deaths in research studies in low-middle income countries","volume":"32","author":"Goldenberg","year":"2019","journal-title":"J. Matern. Neonatal Med."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"7081","DOI":"10.1038\/s41598-021-86445-2","article-title":"Systematic review and meta-analysis of female lifestyle factors and risk of recurrent pregnancy loss","volume":"11","author":"Ng","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Nonyane, B.A.S., Norton, M., Begum, N., Shah, R.M., Mitra, D.K., Darmstadt, G.L., and Baqui, A.H. (2019). Pregnancy intervals after stillbirth, neonatal death and spontaneous abortion and the risk of an adverse outcome in the next pregnancy in rural Bangladesh. BMC Pregnancy Childbirth, 19.","DOI":"10.1186\/s12884-019-2203-0"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Mao, Y.Y., Yang, L., Li, M., Liu, J., Zhu, Q.X., He, Y., and Zhou, W.-J. (2020). Periconceptional folic acid supplementation and the risk of spontaneous abortion among women who prepared to conceive: Impact of supplementation initiation timing. Nutrients, 12.","DOI":"10.3390\/nu12082264"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Yakoob, M.Y., Menezes, E.V., Soomro, T., Haws, R.A., Darmstadt, G.L., and Bhutta, Z.A. (2009). Reducing stillbirths: Behavioural and nutritional interventions before and during pregnancy. BMC Pregnancy Childbirth, 9.","DOI":"10.1186\/1471-2393-9-S1-S3"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e850","DOI":"10.1016\/S2214-109X(22)00126-7","article-title":"Iron, folic acid, and multiple micronutrient supplementation strategies during pregnancy and adverse birth outcomes in Botswana","volume":"10","author":"Caniglia","year":"2022","journal-title":"Lancet Glob Health"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"S91","DOI":"10.1016\/S0140-6736(16)32018-9","article-title":"Folic acid supplementation, birth defects, and adverse pregnancy outcomes in Chinese women: A population-based mega-cohort study","volume":"388","author":"He","year":"2016","journal-title":"Lancet"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.pbj.2017.05.006","article-title":"The impact of folic acid supplementation on gestational and long term health: Critical temporal windows, benefits and risks","volume":"2","author":"Silva","year":"2017","journal-title":"Porto Biomed. J."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Compa\u00f1 Gabucio, L.M., Garc\u00eda de la Hera, M., Torres Collado, L., Fern\u00e1ndez-Somoano, A., Tard\u00f3n, A., Guxens, M., Vrijheid, M., Rebagliato, M., Murcia, M., and Ibarluzea, J. (2021). The use of lower or higher than recommended doses of folic acid supplements during pregnancy is associated with child attentional dysfunction at 4\u20135 years of age in the inma project. Nutrients, 13.","DOI":"10.3390\/nu13020327"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Compa\u00f1-Gabucio, L.M., Torres-Collado, L., Garcia-de la Hera, M., Fern\u00e1ndez-Somoano, A., Tard\u00f3n, A., Julvez, J., Sunyer, J., Rebagliato, M., Murcia, M., and Ibarluzea, J. (2022). Association between the Use of Folic Acid Supplements during Pregnancy and Children\u2019s Cognitive Function at 7\u20139 Years of Age in the INMA Cohort Study. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph191912123"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1017\/S0029665118002689","article-title":"Maternal folate nutrition and offspring health: Evidence and current controversies","volume":"78","author":"Caffrey","year":"2019","journal-title":"Proc. Nutr. Soc."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1038\/s41421-022-00512-0","article-title":"Excess folic acid intake increases DNA de novo point mutations","volume":"9","author":"Cao","year":"2023","journal-title":"Cell Discov."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Alnabbat, K.I., Fardous, A.M., Shahab, A., James, A.A., Bahry, M.R., and Heydari, A.R. (2022). High Dietary Folic Acid Intake Is Associated with Genomic Instability in Peripheral Lymphocytes of Healthy Adults. Nutrients, 14.","DOI":"10.20944\/preprints202208.0429.v1"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2594","DOI":"10.1007\/s00125-016-4086-1","article-title":"A longitudinal study of depression and gestational diabetes in pregnancy and the postpartum period","volume":"59","author":"Hinkle","year":"2016","journal-title":"Diabetologia"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Li, L.J., Wang, X., Chong, Y.S., Chan, J.K.Y., Tan, K.H., Eriksson, J.G., Huang, Z., Rahman, M.L., Cui, L., and Zhang, C. (2023). Exploring preconception signatures of metabolites in mothers with gestational diabetes mellitus using a non-targeted approach. BMC Med., 21.","DOI":"10.1186\/s12916-023-02819-5"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"He, J., Jiang, D., Cui, X., and Ji, C. (2022). Vitamin B12 status and folic acid\/vitamin B12 related to the risk of gestational diabetes mellitus in pregnancy: A systematic review and meta-analysis of observational studies. BMC Pregnancy Childbirth, 22.","DOI":"10.1186\/s12884-022-04911-9"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"2042","DOI":"10.1002\/fsn3.2173","article-title":"Association between maternal folate status and gestational diabetes mellitus","volume":"9","author":"Yang","year":"2021","journal-title":"Food Sci. Nutr."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1016\/j.clnu.2017.03.022","article-title":"High folate and low vitamin B12 status during pregnancy is associated with gestational diabetes mellitus","volume":"37","author":"Lai","year":"2018","journal-title":"Clin. Nutr."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/EBC20150001","article-title":"Introduction to biosensors","volume":"60","author":"Bhalla","year":"2016","journal-title":"Essays Biochem."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Lim, W.Y., Lan, B.L., and Ramakrishnan, N. (2021). Emerging biosensors to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2): A review. Biosensors, 11.","DOI":"10.3390\/bios11110434"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/0022-0728(91)85539-2","article-title":"Electrochemical pretreatment of carbon fibre microelectrodes for the determination of folic acid","volume":"307","author":"Garcia","year":"1991","journal-title":"J. Electroanal. Chem. Interfacial Electrochem."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/S0022-0728(02)00836-7","article-title":"The direct electrochemistry of folic acid at a 2-mercaptobenzothiazole self-assembled gold electrode","volume":"527","author":"Wan","year":"2002","journal-title":"J. Electroanal. Chem."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1007\/s00604-005-0437-1","article-title":"Voltammetric Determination of Folic Acid with a Multi-Walled Carbon Nanotube-Modified Gold Electrode","volume":"152","author":"Wei","year":"2006","journal-title":"Microchim. Acta"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1959","DOI":"10.1002\/elan.200703969","article-title":"Determination of Folic Acid by Adsorptive Stripping Voltammetry at a Lead Film Electrode","volume":"19","author":"Korolczuk","year":"2007","journal-title":"Electroanalysis"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1016\/j.electacta.2007.08.017","article-title":"Electrochemical behavior of folic acid at calixarene based chemically modified electrodes and its determination by adsorptive stripping voltammetry","volume":"53","author":"Vaze","year":"2007","journal-title":"Electrochim. Acta"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"3575","DOI":"10.1016\/j.bios.2009.05.017","article-title":"Selective electrochemical sensor for folic acid at physiological pH using ultrathin electropolymerized film of functionalized thiadiazole modified glassy carbon electrode","volume":"24","author":"Kalimuthu","year":"2009","journal-title":"Biosens. Bioelectron."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1016\/j.bios.2016.05.095","article-title":"MoS2\/reduced graphene oxide as active hybrid material for the electrochemical detection of folic acid in human serum","volume":"85","author":"Chekin","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.bios.2014.03.058","article-title":"Development of \u03b1-polyoxometalate\u2013polypyrrole\u2013Au nanoparticles modified sensor applied for detection of folic acid","volume":"60","author":"Babakhanian","year":"2014","journal-title":"Biosens. Bioelectron."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.jelechem.2014.05.013","article-title":"A novel phosphomolybdic acid\u2013polypyrrole\/graphene composite modified electrode for sensitive determination of folic acid","volume":"726","author":"Wang","year":"2014","journal-title":"J. Electroanal. Chem."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Sun, Y., Wang, X., and Zhang, H. (2022). Sensitive and Stable Electrochemical Sensor for Folic Acid Determination Using a ZIF-67\/AgNWs Nanocomposite. Biosensors, 12.","DOI":"10.3390\/bios12060382"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.snb.2010.02.025","article-title":"Electrochemical sensor for folic acid based on a hyperbranched molecularly imprinted polymer-immobilized sol\u2013gel-modified pencil graphite electrode","volume":"146","author":"Prasad","year":"2010","journal-title":"Sens. Actuators B Chem."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"100235","DOI":"10.1016\/j.sintl.2023.100235","article-title":"An electrochemical biosensor for the determination of folic acid in pregnant women based on DHFR\/c-MWCNTs\/TiO2NPs modified gold electrode","volume":"4","author":"Batra","year":"2023","journal-title":"Sens. Int."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.jelechem.2011.04.023","article-title":"Voltammetric sensor based on ordered mesoporous carbon for folic acid determination","volume":"660","author":"Yang","year":"2011","journal-title":"J. Electroanal. Chem."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1007\/s11581-013-0992-0","article-title":"ZnO nanoparticle-modified ionic liquid-carbon paste electrodefor voltammetric determination of folic acid in food and pharmaceutical samples","volume":"20","author":"Taherkhani","year":"2014","journal-title":"Ionics"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.jelechem.2017.12.017","article-title":"Synthesis of graphene\/ZnO nanowire arrays\/graphene foam and its application for determination of folic acid","volume":"808","author":"Gao","year":"2018","journal-title":"J. Electroanal. Chem."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"106403","DOI":"10.1016\/j.microc.2021.106403","article-title":"Shock waves exposed \u03b1-Fe2O3 nanoparticles for electrochemical sensing of riboflavin, uric acid and folic acid","volume":"168","author":"Meenakshi","year":"2021","journal-title":"Microchem. J."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/j.snb.2008.06.037","article-title":"Single-walled carbon nanotube-ionic liquid paste electrode for the sensitive voltammetric determination of folic acid","volume":"134","author":"Xiao","year":"2008","journal-title":"Sens. Actuators B Chem."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"5622","DOI":"10.1007\/s11694-021-01128-w","article-title":"A glassy carbon electrode modified based on molybdenum disulfide for determination of folic acid in the real samples","volume":"15","author":"Alizadeh","year":"2021","journal-title":"J. Food Meas. Charact."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"114694","DOI":"10.1016\/j.ecoenv.2023.114694","article-title":"Synergetic effects of Mo2C sphere\/SCN nanocatalysts interface for nanomolar detection of uric acid and folic acid in presence of interferences","volume":"253","author":"Ganesh","year":"2023","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1007\/s12678-022-00756-0","article-title":"Folic Acid Determination in Food Samples Using Green Synthesized Copper Oxide Nanoparticles and Electro-Poly (Methyl Orange) Sensor","volume":"13","author":"Hasan","year":"2022","journal-title":"Electrocatalysis"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1016\/j.msec.2012.12.090","article-title":"Highly selective electrochemical biosensor for the determination of folic acid based on DNA modified-pencil graphite electrode using response surface methodology","volume":"33","author":"Mirmoghtadaie","year":"2013","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"295501","DOI":"10.1088\/0957-4484\/25\/29\/295501","article-title":"Fabrication of folic acid sensor based on the Cu doped SnO2 nanoparticles modified glassy carbon electrode","volume":"25","author":"Lavanya","year":"2014","journal-title":"Nanotechnology"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1016\/j.electacta.2014.11.069","article-title":"Facile one-step direct electrodeposition of bismuth nanowires on glassy carbon electrode for selective determination of folic acid","volume":"151","author":"Ananthi","year":"2015","journal-title":"Electrochim. Acta"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.saa.2014.07.087","article-title":"Development of electrochemical folic acid sensor based on hydroxyapatite nanoparticles","volume":"137","author":"Kanchana","year":"2015","journal-title":"Spectrochim. Acta A Mol. Biomol. Spectrosc."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.jelechem.2018.04.043","article-title":"Selective and sensitive voltammetric determination of folic acid using graphite\/restricted access molecularly imprinted poly(methacrylic acid)\/SiO2 composite","volume":"818","author":"Kuceki","year":"2018","journal-title":"J. Electroanal. Chem."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.snb.2014.03.075","article-title":"BSA-modified gold nanoclusters for sensing of folic acid","volume":"199","author":"Hemmateenejad","year":"2014","journal-title":"Sens. Actuators B Chem."},{"key":"ref_98","first-page":"87","article-title":"Electrochemical Behavior of Folic Acid at A Boron-Doped Diamond Electrode: Its Adsorptive Stripping Voltammetric Determination in Tablets","volume":"11","author":"Yardim","year":"2014","journal-title":"Turk. J. Pharm. Sci."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.snb.2015.08.065","article-title":"Delicate photoelectrochemical sensor for folic acid based on carbon nanohorns supported interwoven titanate nanotubes","volume":"222","author":"Dai","year":"2016","journal-title":"Sens. Actuators B Chem."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.snb.2010.09.011","article-title":"New strategy for simultaneous and selective voltammetric determination of norepinephrine, acetaminophen and folic acid using ZrO2 nanoparticles-modified carbon paste electrode","volume":"151","author":"Beitollahi","year":"2010","journal-title":"Sens. Actuators B Chem."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/S0308-8146(00)00115-1","article-title":"Biosensor-based determination of folic acid in fortified food","volume":"70","author":"Lindeberg","year":"2000","journal-title":"Food Chem."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1002\/bio.1145","article-title":"Determination of folic acid by high-performance liquid chromatography with direct electrogenerated chemiluminescence reaction","volume":"25","author":"Sun","year":"2010","journal-title":"Luminescence"},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/j.saa.2012.11.078","article-title":"Chemiluminescence determination of folic acid by a flow injection analysis assembly","volume":"105","author":"Wabaidur","year":"2013","journal-title":"Spectrochim. Acta A Mol. Biomol. Spectrosc."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.saa.2018.11.051","article-title":"Copper nanoclusters-enhanced chemiluminescence for folic acid and nitrite detection","volume":"210","author":"Han","year":"2019","journal-title":"Spectrochim. Acta A Mol. Biomol. Spectrosc."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"2444","DOI":"10.1007\/s12161-017-0804-7","article-title":"Development of a Simple, Fast, and Quantitative Lateral Flow Immunochromatographic Strip for Folic Acid","volume":"10","author":"Liang","year":"2017","journal-title":"Food Anal. Methods"},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"8152","DOI":"10.1039\/D2NJ00601D","article-title":"A red luminescent europium metal organic framework (Eu-MOF) integrated with a paper strip using smartphone visual detection for determination of folic acid in pharmaceutical formulations","volume":"46","author":"Kayani","year":"2022","journal-title":"New J. Chem."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1007\/s00604-019-3705-1","article-title":"Determination of folic acid via its quenching effect on the fluorescence of MoS2 quantum dots","volume":"186","author":"Peng","year":"2019","journal-title":"Microchim. Acta"},{"key":"ref_108","doi-asserted-by":"crossref","unstructured":"Novichikhin, D.O., Orlov, A.V., Antopolsky, M.L., Znoyko, S.L., and Nikitin, P.I. (2023). Specific and Sensitive Determination of Folic Acid by Label-Free Chemosensors with Microscope Glass Slips as Single-Use Consumables. Chemosensors, 11.","DOI":"10.3390\/chemosensors11010017"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"2805","DOI":"10.1007\/s00216-020-02507-w","article-title":"Fluorescent paper\u2013based sensor based on carbon dots for detection of folic acid","volume":"412","author":"Li","year":"2020","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"108470","DOI":"10.1016\/j.microc.2023.108470","article-title":"Ultrafast detection of folic acid in nanomolar levels and cancer cell imaging using hydrothermally synthesized carbon dots","volume":"188","author":"Krishnapriya","year":"2023","journal-title":"Microchem. J."}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/13\/10\/912\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:00:48Z","timestamp":1760130048000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/13\/10\/912"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,28]]},"references-count":110,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2023,10]]}},"alternative-id":["bios13100912"],"URL":"https:\/\/doi.org\/10.3390\/bios13100912","relation":{},"ISSN":["2079-6374"],"issn-type":[{"value":"2079-6374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,28]]}}}