{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T23:12:42Z","timestamp":1782947562174,"version":"3.54.5"},"reference-count":53,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,2,17]],"date-time":"2024-02-17T00:00:00Z","timestamp":1708128000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","award":["RSPD2023R589"],"award-info":[{"award-number":["RSPD2023R589"]}],"id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>The needless use of tetracyclines (TCs) in foodstuffs is a huge health concern in low- and middle-income and Arab countries. Herein, a sensitive and faster monitoring system for H2O2 and TCs is proposed, utilizing the large surface-to-volume ratio of a non-spherical gold nanoparticle\/black phosphorus nanocomposite (BP-nsAu NPs) for the first time. BP-nsAu NPs were synthesized through a single-step method that presented nanozymatic activity through 3,3\u2032,5,5\u2032-Tetramethylbenzidine (TMB) oxidation while H2O2 was present and obeyed the Michaelis\u2013Menten equation. The nanozymatic activity of the BP-nsAu NPs was enhanced 12-fold and their detection time was decreased 83-fold compared to conventional nanozymatic reactions. The proposed method enabled us to quantify H2O2 with a limit of detection (LOD) value of 60 nM. Moreover, target-specific aptamer-conjugated BP-nsAu NPs helped us detect TCs with an LOD value of 90 nM. The present strategy provides a proficient route for low-level TC monitoring in real samples.<\/jats:p>","DOI":"10.3390\/bios14020106","type":"journal-article","created":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T03:18:38Z","timestamp":1708312718000},"page":"106","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Enhanced Nanozymatic Activity on Rough Surfaces for H2O2 and Tetracycline Detection"],"prefix":"10.3390","volume":"14","author":[{"given":"Tawfiq","family":"Alsulami","sequence":"first","affiliation":[{"name":"Department of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6103-4269","authenticated-orcid":false,"given":"Abdulhakeem","family":"Alzahrani","sequence":"additional","affiliation":[{"name":"Department of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"116006","DOI":"10.1016\/j.trac.2020.116006","article-title":"Recent Biosensing Advances in the Rapid Detection of Illicit Drugs","volume":"131","author":"Ahmed","year":"2020","journal-title":"TrAC Trends Anal. 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