{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:06:05Z","timestamp":1792173965610,"version":"4.4.0"},"reference-count":189,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T00:00:00Z","timestamp":1710374400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Open Project Program of State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement","award":["XZNKY-CZ-2022-016-08"],"award-info":[{"award-number":["XZNKY-CZ-2022-016-08"]}]},{"name":"Open Project Program of State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement","award":["XZ202101ZD0002N"],"award-info":[{"award-number":["XZ202101ZD0002N"]}]},{"name":"Major science and technology projects during the 14th Five Year Plan Period","award":["XZNKY-CZ-2022-016-08"],"award-info":[{"award-number":["XZNKY-CZ-2022-016-08"]}]},{"name":"Major science and technology projects during the 14th Five Year Plan Period","award":["XZ202101ZD0002N"],"award-info":[{"award-number":["XZ202101ZD0002N"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>The rapid and accurate identification of parasites is crucial for prompt therapeutic intervention in parasitosis and effective epidemiological surveillance. For accurate and effective clinical diagnosis, it is imperative to develop a nucleic-acid-based diagnostic tool that combines the sensitivity and specificity of nucleic acid amplification tests (NAATs) with the speed, cost-effectiveness, and convenience of isothermal amplification methods. A new nucleic acid detection method, utilizing the clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) nuclease, holds promise in point-of-care testing (POCT). CRISPR\/Cas12a is presently employed for the detection of Plasmodium falciparum, Toxoplasma gondii, Schistosoma haematobium, and other parasites in blood, urine, or feces. Compared to traditional assays, the CRISPR assay has demonstrated notable advantages, including comparable sensitivity and specificity, simple observation of reaction results, easy and stable transportation conditions, and low equipment dependence. However, a common issue arises as both amplification and cis-cleavage compete in one-pot assays, leading to an extended reaction time. The use of suboptimal crRNA, light-activated crRNA, and spatial separation can potentially weaken or entirely eliminate the competition between amplification and cis-cleavage. This could lead to enhanced sensitivity and reduced reaction times in one-pot assays. Nevertheless, higher costs and complex pre-test genome extraction have hindered the popularization of CRISPR\/Cas12a in POCT.<\/jats:p>","DOI":"10.3390\/bios14030145","type":"journal-article","created":{"date-parts":[[2024,3,15]],"date-time":"2024-03-15T04:47:05Z","timestamp":1710478025000},"page":"145","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Detection of Parasites in the Field: The Ever-Innovating CRISPR\/Cas12a"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6423-3060","authenticated-orcid":false,"given":"Xin","family":"Li","sequence":"first","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhisheng","family":"Dang","sequence":"additional","affiliation":[{"name":"National Institute of Parasitic Diseases, Chinese Center for Diseases Control and Prevention (Chinese Center for Tropical Diseases Research), Key Laboratory of Parasite and Vector Biology, National Health Commission of the People\u2019s Republic of China (NHC), World Health Organization (WHO) Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai 200025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqiang","family":"Tang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement, Lhasa 850002, China"},{"name":"Tibet Academy of Agriculture and Animal Husbandry Sciences, Lhasa 850002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoji","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9548-3831","authenticated-orcid":false,"given":"Jianwei","family":"Shao","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3613-2350","authenticated-orcid":false,"given":"Fuqiang","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.1016\/S0140-6736(04)16412-X","article-title":"Toxoplasmosis","volume":"363","author":"Montoya","year":"2004","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Theel, E.S., and Pritt, B.S. 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