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The material combines the advantageous properties of clay with those of the chitosan derivative; has improved water solubility and reduced molecular weight and viscosity; involves an eco-friendly synthesis; and exhibits ion exchange capacity, good adhesiveness, and a large specific surface area for enzyme adsorption. The physicochemical characteristics of the composite were analyzed by infrared spectroscopy and X-ray diffraction to determine clay\u2013polycation interactions. The electrochemical response of the different polyphenols to glassy carbon electrodes modified with the composite was evaluated by cyclic voltammetry. The sensitivity and detection limit values obtained with the biosensor toward hydroquinone, chlorogenic acid, catechol, and resorcinol are (1.6 \u00b1 0.2) \u00d7 102 \u00b5A mM\u22121 and (74 \u00b1 8) nM; (1.2 \u00b1 0.1) \u00d7 102 \u00b5A mM\u22121 and (26 \u00b1 3) nM; (16 \u00b1 2) \u00b5A mM\u22121 and (0.74 \u00b1 0.09) \u03bcM; and (3.7\u00b1 0.3) \u00b5A mM\u22121 and (3.3 \u00b1 0.2) \u03bcM, respectively. The biosensor was applied to quantify polyphenols in pennyroyal and lemon verbena extracts.<\/jats:p>","DOI":"10.3390\/bios14060278","type":"journal-article","created":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T06:58:07Z","timestamp":1716965887000},"page":"278","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Immobilization of Horseradish Peroxidase onto Montmorillonite\/Glucosamine\u2013Chitosan Composite for Electrochemical Biosensing of Polyphenols"],"prefix":"10.3390","volume":"14","author":[{"given":"Mar\u00eda Bel\u00e9n","family":"Piccoli","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones en Fisicoqu\u00edmica de C\u00f3rdoba (INFIQC-UNC-CONICET), Departamento de Fisicoqu\u00edmica, Facultad de Ciencias Qu\u00edmicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba X5000HUA, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florencia Alejandra","family":"Gulotta","sequence":"additional","affiliation":[{"name":"Independent Researcher, Santiago del Estero G4206XCP, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Ang\u00e9lica","family":"Montenegro","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones y Transferencia de Villa Mar\u00eda (CIT-VM), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Universidad Nacional de Villa Mar\u00eda (UNVM), Villa Mar\u00eda X5220XAO, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3402-417X","authenticated-orcid":false,"given":"Noelia Luciana","family":"Vanden Braber","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones y Transferencia de Villa Mar\u00eda (CIT-VM), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Universidad Nacional de Villa Mar\u00eda (UNVM), Villa Mar\u00eda X5220XAO, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f3nica Irene","family":"Paz Zanini","sequence":"additional","affiliation":[{"name":"Instituto de Bionanotecnolog\u00eda del NOA (INBIONATEC), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas CONICET, Universidad Nacional de Santiago del Estero (UNSE), Santiago del Estero G4206XCP, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy Fabiana","family":"Ferreyra","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Fisicoqu\u00edmica de C\u00f3rdoba (INFIQC-UNC-CONICET), Departamento de Fisicoqu\u00edmica, Facultad de Ciencias Qu\u00edmicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba X5000HUA, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1125106","DOI":"10.3389\/fnut.2023.1125106","article-title":"Extraction, Characterization of Polyphenols from Certain Medicinal Plants and Evaluation of Their Antioxidant, Antitumor, Antidiabetic, Antimicrobial Properties, and Potential Use in Human Nutrition","volume":"10","author":"Mueed","year":"2023","journal-title":"Front. 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