{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T19:26:55Z","timestamp":1787858815441,"version":"build-2784847793"},"reference-count":34,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T00:00:00Z","timestamp":1716940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["LY24F010008"],"award-info":[{"award-number":["LY24F010008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>As a typical biomarker of Alzheimer\u2019s disease, rapid and specific detection of tau protein can help improve the early diagnosis and prognosis of the disease. In this study, a simple sandwich electrochemical immunosensor was developed for rapid detection of tau protein. Primary monoclonal antibodies (mAb1) against the middle domain of tau protein (amino acids 189\u2013195) were immobilized on the gold electrode surface through a self-assembled monolayer (SAM) of 3,3\u2032-dithiobis (sulfosuccinimidyl propionate) (DTSSP). Then the tau protein was captured through the specific adsorption between the antigen and the antibody, resulting in a change in the impedance. Secondary monoclonal antibodies (mAb2) against the N-terminal region of tau protein were used for further amplification of the binding reaction between mAb1 and tau protein. A linear correlation between the total change in impedance and the logarithm of tau concentration was found from 2 \u00d7 10\u22126 mg mL\u22121 to 2 \u00d7 10\u22123 mg mL\u22121, with a detection limit as low as 1 \u00d7 10\u22126 mg mL\u22121. No significant interference was observed from human serum albumin. Furthermore, the fabricated sandwich immunosensor successfully detected target tau protein in artificial cerebrospinal fluid (aCSF) samples, indicating good potential for clinical applications in the future.<\/jats:p>","DOI":"10.3390\/bios14060279","type":"journal-article","created":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T06:08:14Z","timestamp":1717049294000},"page":"279","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A Simple Sandwich Electrochemical Immunosensor for Rapid Detection of the Alzheimer\u2019s Disease Biomarker Tau Protein"],"prefix":"10.3390","volume":"14","author":[{"given":"Mingzhu","family":"Yang","sequence":"first","affiliation":[{"name":"Ministry of Education Engineering Research Center of Smart Microsensors and Microsystems, College of Electronics and Information, Hangzhou Dianzi University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihong","family":"Chen","sequence":"additional","affiliation":[{"name":"Zhejiang College of Construction, Hangzhou 311231, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyu","family":"Sun","sequence":"additional","affiliation":[{"name":"Ministry of Education Engineering Research Center of Smart Microsensors and Microsystems, College of Electronics and Information, Hangzhou Dianzi University, Hangzhou 310018, China"},{"name":"School of Automation, Hangzhou Dianzi University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dujuan","family":"Li","sequence":"additional","affiliation":[{"name":"Ministry of Education Engineering Research Center of Smart Microsensors and Microsystems, College of Electronics and Information, Hangzhou Dianzi University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanbin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"101587","DOI":"10.1016\/j.arr.2022.101587","article-title":"Salivary biomarkers of neurodegenerative and demyelinating diseases and biosensors for their detection","volume":"76","author":"Goldoni","year":"2022","journal-title":"Ageing Res. 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