{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:48:20Z","timestamp":1768783700782,"version":"3.49.0"},"reference-count":35,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T00:00:00Z","timestamp":1719273600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Research, Innovation and Digitization","award":["PN-III-P2-2.1-PED-2021-3693"],"award-info":[{"award-number":["PN-III-P2-2.1-PED-2021-3693"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>The development of low-cost, sensitive, and simple analytical tools for biomolecule detection in health status monitoring is nowadays a growing research topic. Sensing platforms integrating nanocomposite materials as recognition elements in the monitoring of various biomolecules and biomarkers are addressing this challenging objective. Herein, we have developed electrochemical sensing platforms by means of a novel fabrication procedure for biomolecule detection. The platforms are based on commercially available low-cost conductive substrates like glassy carbon and\/or screen-printed carbon electrodes selectively functionalized with nanocomposite materials composed of Ag and Au metallic nanoparticles and an organic polymer, poly(3,4-ethylenedioxythiophene). The novel fabrication method made use of alternating currents with controlled amplitude and frequency. The frequency of the applied alternating current was 100 mHz for the polymer deposition, while a frequency value of 50 mHz was used for the in situ electrodeposition of Ag and Au nanoparticles. The selected frequency values ensured the successful preparation of the composite materials. The use of readily available composite materials is intended to produce cost-effective analytical tools. The judicious modification of the organic conductive matrix by various metallic nanoparticles, such as Ag and Au, extends the potential applications of the sensing platform toward a range of biomolecules like quercetin and epinephrine, chosen as benchmark analytes for proof-of-concept antioxidant and neurotransmitter detection. The sensing platforms were tested successfully for quercetin and epinephrine determination on synthetic and real samples. Wide linear response ranges and low limit-of-detection values were obtained for epinephrine and quercetin detection.<\/jats:p>","DOI":"10.3390\/bios14070320","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T12:46:56Z","timestamp":1719319616000},"page":"320","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["MeNPs-PEDOT Composite-Based Detection Platforms for Epinephrine and Quercetin"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2754-3550","authenticated-orcid":false,"given":"Sorina Alexandra","family":"Leau","sequence":"first","affiliation":[{"name":"Department of Electrochemistry and Corrosion, Institute of Physical Chemistry\u2013Ilie Murgulescu of the Romanian Academy, 202 Splaiul Independentei, 060021 Bucharest, Romania"},{"name":"Department of Analytical Chemistry and Environmental Engineering, Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology Politehnica of Bucharest, 1-7 Polizu Gheorghe, 011061 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9898-0796","authenticated-orcid":false,"given":"Mariana","family":"Marin","sequence":"additional","affiliation":[{"name":"Department of Electrochemistry and Corrosion, Institute of Physical Chemistry\u2013Ilie Murgulescu of the Romanian Academy, 202 Splaiul Independentei, 060021 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5061-2617","authenticated-orcid":false,"given":"Ana Maria","family":"Toader","sequence":"additional","affiliation":[{"name":"Department of Electrochemistry and Corrosion, Institute of Physical Chemistry\u2013Ilie Murgulescu of the Romanian Academy, 202 Splaiul Independentei, 060021 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4865-2652","authenticated-orcid":false,"given":"Mihai","family":"Anastasescu","sequence":"additional","affiliation":[{"name":"Department of Electrochemistry and Corrosion, Institute of Physical Chemistry\u2013Ilie Murgulescu of the Romanian Academy, 202 Splaiul Independentei, 060021 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9830-8246","authenticated-orcid":false,"given":"Cristian","family":"Matei","sequence":"additional","affiliation":[{"name":"Department of Inorganic Chemistry, Physical Chemistry and Electrochemistry, Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology Politehnica of Bucharest, 1-7 Polizu Gheorghe, 011061 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilia","family":"Lete","sequence":"additional","affiliation":[{"name":"Department of Electrochemistry and Corrosion, Institute of Physical Chemistry\u2013Ilie Murgulescu of the Romanian Academy, 202 Splaiul Independentei, 060021 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stelian","family":"Lupu","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry and Environmental Engineering, Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology Politehnica of Bucharest, 1-7 Polizu Gheorghe, 011061 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Wen, X., Yang, X., Ge, Z., Ma, H., Wang, R., Tian, F., Teng, P., Gao, S., Li, K., and Zhang, B. (2024). Self-powered optical fiber biosensor integrated with enzymes for non-invasive glucose sensing. Biosens. Bioelectron., 253.","DOI":"10.1016\/j.bios.2024.116191"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"125937","DOI":"10.1016\/j.talanta.2024.125937","article-title":"Electrochemical sensing mechanisms of neonicotinoid pesticides and recent progress in utilizing functional materials for electrochemical detection platforms","volume":"273","author":"Ding","year":"2024","journal-title":"Talanta"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"215746","DOI":"10.1016\/j.ccr.2024.215746","article-title":"Design and engineering of 2D MXenes for point-of-care electrochemical detection of bioactive analytes and environmental pollutants","volume":"507","author":"Mohanapriya","year":"2024","journal-title":"Coord. Chem. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"8007","DOI":"10.1039\/D3RA08567H","article-title":"Enhanced sensitivity in electrochemical detection of ochratoxin A within food samples using ferrocene- and aptamer-tethered gold nanoparticles on disposable electrodes","volume":"14","author":"Argoubi","year":"2024","journal-title":"RSC Adv."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"110326","DOI":"10.1016\/j.microc.2024.110326","article-title":"Recent advances in nanostructured conducting polymer electrospun for application in electrochemical biosensors","volume":"200","author":"Daweshar","year":"2024","journal-title":"Microchem. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"037557","DOI":"10.1149\/1945-7111\/ab6e5f","article-title":"Review-Functional platforms for (bio)sensing: Thiophene-pyrrole hybrid polymers","volume":"167","author":"Apetrei","year":"2020","journal-title":"J. Electrochem. Soc."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1007\/s00604-022-05490-4","article-title":"Electrochemical assay of acetamiprid in vegetables based on nitrogen-doped graphene\/polypyrrole nanocomposites","volume":"189","author":"Wang","year":"2022","journal-title":"Microchim. Acta"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1007\/s42823-023-00648-3","article-title":"ZIF-8\/CNFs\/PANI composite as an electrochemical platform in trace-level nitrite sensing","volume":"34","author":"Patri","year":"2024","journal-title":"Carbon Lett."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.jelechem.2014.12.013","article-title":"Poly(3,4-ethylenedioxythiophene) nanorods grown on graphene oxide sheets as electrochemical sensing platform for rutin","volume":"739","author":"Zhang","year":"2015","journal-title":"J. Electroanal. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.bios.2016.08.006","article-title":"Tyramine detection using PEDOT:PSS\/AuNPs\/1-methyl-4-mercaptopyridine modified screen-printed carbon electrode with molecularly imprinted polymer solid phase extraction","volume":"87","author":"Li","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Rodr\u00edguez-Garc\u00eda, C., S\u00e1nchez-Quesada, C., and Gaforio, J.J. (2019). Dietary flavonoids as cancer chemopreventive agents: An updated review of human studies. Antioxidants, 8.","DOI":"10.3390\/antiox8050137"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Qi, W., Qi, W., Xiong, D., and Long, M. (2022). Quercetin: Its antioxidant mechanism, antibacterial properties and potential application in prevention and control of toxipathy. Molecules, 27.","DOI":"10.3390\/molecules27196545"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Xu, D., Hu, M.-J., Wang, Y.-Q., and Cui, Y.-L. (2019). Antioxidant activities of quercetin and its complexes for medicinal application. Molecules, 24.","DOI":"10.3390\/molecules24061123"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Carvalho, D., Jesus, \u00c2., Pinho, C., Oliveira, R.F., Moreira, F., and Oliveira, A.I. (2023). Validation of an HPLC-DAD method for quercetin quantification in nanoparticles. Pharmaceuticals, 16.","DOI":"10.3390\/ph16121736"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1007\/s00604-020-04587-y","article-title":"Gold nanoparticle-graphene quantum dots nanozyme for the wide range and sensitive electrochemical determination of quercetin in plasma droplets","volume":"187","author":"Stefanov","year":"2020","journal-title":"Microchim. Acta"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"138405","DOI":"10.1016\/j.foodchem.2024.138405","article-title":"Synchronous activation of praseodymium vanadate\/graphitic carbon nitride nanocomposite: A promising electrode material for detection of flavonoids-quercetin","volume":"441","author":"Bharathi","year":"2024","journal-title":"Food Chem."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Xu, X., Zuo, Y., Chen, S., Hatami, A., and Gu, H. (2024). Advancements in brain research: The in vivo\/in vitro electrochemical detection of neurochemicals. Biosensors, 14.","DOI":"10.3390\/bios14030125"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/j.talanta.2016.06.066","article-title":"Electrochemical sensors and biosensors for determination of catecholamine neurotransmitters: A review","volume":"160","author":"Ribeiro","year":"2016","journal-title":"Talanta"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"116541","DOI":"10.1016\/j.trac.2022.116541","article-title":"Recent trends in carbon-based microelectrodes as electrochemical sensors for neurotransmitter detection: A review","volume":"148","author":"Liu","year":"2022","journal-title":"Trend. Anal. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Wang, L., Hu, Y., Jiang, N., and Yetisen, A.K. (2024). Biosensors for psychiatric biomarkers in mental health monitoring. Biosens. Bioelectron., 256.","DOI":"10.1016\/j.bios.2024.116242"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"126178","DOI":"10.1016\/j.talanta.2024.126178","article-title":"A simple and highly sensitive flexible sensor with extended-gate field-effect transistor for epinephrine detection utilizing InZnSnO sensing films","volume":"275","author":"Pan","year":"2024","journal-title":"Talanta"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Leau, S.A., Lete, C., Matei, C., and Lupu, S. (2023). Electrochemical sensing platform based on metal nanoparticles for epinephrine and serotonin. Biosensors, 13.","DOI":"10.3390\/bios13080781"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3043","DOI":"10.1007\/s10008-016-3270-z","article-title":"Electrochemical and microgravimetric studies of poly [3,4-ethylenedioxythiophene]-tyrosinase biocomposite material electrodeposited onto gold electrodes by a sinusoidal voltages method","volume":"20","author":"Lete","year":"2016","journal-title":"J. Solid State Electrochem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"116774","DOI":"10.1016\/j.jelechem.2022.116774","article-title":"Facile design of wolframite type CoWO4 nanoparticles: A selective and simultaneous electrochemical detection of quercetin and rutin","volume":"922","author":"Vinothkumar","year":"2022","journal-title":"J. Electroanal. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"117946","DOI":"10.1016\/j.jelechem.2023.117946","article-title":"Rational design of praseodymium ferrite decorated on reduced graphene oxide for the electrochemical detection of quercetin","volume":"951","author":"Ruspika","year":"2023","journal-title":"J. Electroanal. Chem."},{"key":"ref_26","unstructured":"Frisch, M.J., Trucks, G.W., Schlegel, H.B., Scuseria, G.E., Robb, M.A., Cheeseman, J.R., Scalmani, G., Barone, V., Mennucci, B., and Petersson, G.A. (2009). Gaussian 09, Gaussian, Inc."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"6104","DOI":"10.1063\/1.470438","article-title":"Extension of Gaussian-2 theory to molecules containing third-row atoms Ga-Kr","volume":"103","author":"Curtiss","year":"1995","journal-title":"J. Chem. Phys."},{"key":"ref_28","first-page":"63","article-title":"Structural analysis and multipole modelling of quercetin monohydrate\u2014 a quantitative and comparative study","volume":"B67","author":"Munshi","year":"2011","journal-title":"Acta Cryst."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/j.electacta.2019.04.188","article-title":"A simple, highly sensitive and stable electrochemical sensor for the detection of quercetin in solution, onion and honey buckwheat using zinc oxide supported on carbon nanosheet (ZnO\/CNS\/MCPE) modified carbon paste electrode","volume":"313","author":"Saritha","year":"2019","journal-title":"Electrochim. Acta"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"221147","DOI":"10.20964\/2022.11.53","article-title":"Electrochemical sensors based on Fe@Fe2O3 nanowires, N-doped ZnO and Au nanoparticles for quercetin determination","volume":"17","author":"Xie","year":"2022","journal-title":"Int. J. Electrochem. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"100108","DOI":"10.1016\/j.hybadv.2023.100108","article-title":"Rapid electrochemical detection of epinephrine in human serum based on polydopamine-wrapped cerium oxide nanocomposite","volume":"4","author":"Yadav","year":"2023","journal-title":"Hybrid Adv."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/s12678-022-00769-9","article-title":"Electrochemical sensing of epinephrine on a carbon nanofibers and gold nanoparticle-modified electrode","volume":"14","author":"Sipuka","year":"2023","journal-title":"Electrocatalysis"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1007\/s00604-023-06158-3","article-title":"Lab-made disposable screen-printed electrochemical sensors and immunosensors modified with Pd nanoparticles for Parkinson\u2019s disease diagnostics","volume":"191","author":"Orzari","year":"2024","journal-title":"Microchim. Acta"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"124866","DOI":"10.1007\/s11356-022-23660-y","article-title":"Voltammetric analysis of epinephrine using glassy carbon electrode modified with nanocomposite prepared from co-nd bimetallic nanoparticles, alumina nanoparticles and functionalized multiwalled carbon nanotubes","volume":"30","author":"Agrahari","year":"2023","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"116204","DOI":"10.1016\/j.jelechem.2022.116204","article-title":"A combined experimental-computational approach for electrocatalytic detection of epinephrine using nanocomposite sensor based on polyaniline\/nickel oxide","volume":"911","author":"Uwaya","year":"2022","journal-title":"J. Electroanal. Chem."}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/14\/7\/320\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:04:17Z","timestamp":1760108657000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/14\/7\/320"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,25]]},"references-count":35,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2024,7]]}},"alternative-id":["bios14070320"],"URL":"https:\/\/doi.org\/10.3390\/bios14070320","relation":{},"ISSN":["2079-6374"],"issn-type":[{"value":"2079-6374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,25]]}}}