{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:34:19Z","timestamp":1787027659383,"version":"build-2736575974"},"reference-count":119,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T00:00:00Z","timestamp":1721433600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ITMO Cancer of Aviesan","award":["22CP174-00"],"award-info":[{"award-number":["22CP174-00"]}]},{"name":"ITMO Cancer of Aviesan","award":["23-75-30016"],"award-info":[{"award-number":["23-75-30016"]}]},{"name":"ITMO Cancer of Aviesan","award":["21-79-30048"],"award-info":[{"award-number":["21-79-30048"]}]},{"name":"Russian Science Foundation (RSF)","award":["22CP174-00"],"award-info":[{"award-number":["22CP174-00"]}]},{"name":"Russian Science Foundation (RSF)","award":["23-75-30016"],"award-info":[{"award-number":["23-75-30016"]}]},{"name":"Russian Science Foundation (RSF)","award":["21-79-30048"],"award-info":[{"award-number":["21-79-30048"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>The prevalence of allergic diseases has increased tremendously in recent decades, which can be attributed to growing exposure to environmental triggers, changes in dietary habits, comorbidity, and the increased use of medications. In this context, the multiplexed diagnosis of sensitization to various allergens and the monitoring of the effectiveness of treatments for allergic diseases become particularly urgent issues. The detection of allergen-specific antibodies, in particular, sIgE and sIgG, is a modern alternative to skin tests due to the safety and efficiency of this method. The use of allergen microarrays to detect tens to hundreds of allergen-specific antibodies in less than 0.1 mL of blood serum enables the transition to a deeply personalized approach in the diagnosis of these diseases while reducing the invasiveness and increasing the informativeness of analysis. This review discusses the technological approaches underlying the development of allergen microarrays and other protein microarrays, including the methods of selection of the microarray substrates and matrices for protein molecule immobilization, the obtainment of allergens, and the use of different types of optical labels for increasing the sensitivity and specificity of the detection of allergen-specific antibodies.<\/jats:p>","DOI":"10.3390\/bios14070353","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T14:45:53Z","timestamp":1721659553000},"page":"353","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Allergen Microarrays and New Physical Approaches to More Sensitive and Specific Detection of Allergen-Specific Antibodies"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5321-3753","authenticated-orcid":false,"given":"Pavel","family":"Sokolov","sequence":"first","affiliation":[{"name":"Life Improvement by Future Technologies (LIFT) Center, 143025 Moscow, Russia"},{"name":"Laboratory of Nano-Bioengineering, National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 115409 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Evsegneeva","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology and Allergology, Institute of Molecular Medicine, Sechenov First Moscow State Medical University (Sechenov University), 119146 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1930-5424","authenticated-orcid":false,"given":"Alexander","family":"Karaulov","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology and Allergology, Institute of Molecular Medicine, Sechenov First Moscow State Medical University (Sechenov University), 119146 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2796-7898","authenticated-orcid":false,"given":"Alyona","family":"Sukhanova","sequence":"additional","affiliation":[{"name":"Laboratoire BioSpecT, Universit\u00e9 de Reims Champagne-Ardenne, 51100 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8391-040X","authenticated-orcid":false,"given":"Igor","family":"Nabiev","sequence":"additional","affiliation":[{"name":"Life Improvement by Future Technologies (LIFT) Center, 143025 Moscow, Russia"},{"name":"Laboratory of Nano-Bioengineering, National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 115409 Moscow, Russia"},{"name":"Department of Clinical Immunology and Allergology, Institute of Molecular Medicine, Sechenov First Moscow State Medical University (Sechenov University), 119146 Moscow, Russia"},{"name":"Laboratoire BioSpecT, Universit\u00e9 de Reims Champagne-Ardenne, 51100 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5114\/ada.2022.119009","article-title":"The increase in allergic conditions based on a review of literature","volume":"40","year":"2023","journal-title":"Postep. 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