{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T22:37:19Z","timestamp":1782167839526,"version":"3.54.5"},"reference-count":50,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T00:00:00Z","timestamp":1724025600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of South Korea","award":["NRF-2023R1A2C2004964"],"award-info":[{"award-number":["NRF-2023R1A2C2004964"]}]},{"name":"Ministry of Health and Welfare","award":["KH140292"],"award-info":[{"award-number":["KH140292"]}]},{"name":"The Ministry of Science and ICT","award":["IITP-2024-RS-2023-00258971"],"award-info":[{"award-number":["IITP-2024-RS-2023-00258971"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Quantifying the formation and decomposition of amyloid is a crucial issue in the development of new drugs and therapies for treating amyloidosis. The current technologies for grasping amyloid formation and decomposition include fluorescence analysis using thioflavin-T, secondary structure analysis using circular dichroism, and image analysis using atomic force microscopy or transmission electron microscopy. These technologies typically require spectroscopic devices or expensive nanoscale imaging equipment and involve lengthy analysis, which limits the rapid screening of amyloid-degrading drugs. In this study, we introduce a technology for rapidly assessing amyloid decomposition using capillary flow-based paper (CFP). Amyloid solutions exhibit gel-like physical properties due to insoluble denatured polymers, resulting in a shorter flow distance on CFP compared to pure water. Experimental conditions were established to consistently control the flow distance based on a hen-egg-white lysozyme amyloid solution. It was confirmed that as amyloid is decomposed by trypsin, the flow distance increases on the CFP. Our method is highly useful for detecting changes in the gel properties of amyloid solutions within a minute, and we anticipate its use in the rapid, large-scale screening of anti-amyloid agents in the future.<\/jats:p>","DOI":"10.3390\/bios14080400","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T10:11:28Z","timestamp":1724062288000},"page":"400","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Capillary Flow-Based One-Minute Quantification of Amyloid Proteolysis"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3271-5097","authenticated-orcid":false,"given":"Taeha","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Bioinformatics, Korea University, Sejong 30019, Republic of Korea"},{"name":"Interdisciplinary Graduate Program for Artificial Intelligence Smart Convergence Technology, Korea University, Sejong 30019, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da Yeon","family":"Cheong","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioinformatics, Korea University, Sejong 30019, Republic of Korea"},{"name":"Interdisciplinary Graduate Program for Artificial Intelligence Smart Convergence Technology, Korea University, Sejong 30019, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang Hyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioinformatics, Korea University, Sejong 30019, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2761-1542","authenticated-orcid":false,"given":"Jae Hyun","family":"You","sequence":"additional","affiliation":[{"name":"Department of Digital Management, Korea University, Sejong 30019, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8096-7286","authenticated-orcid":false,"given":"Jinsung","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon 16419, Republic of Korea"},{"name":"Department of MetaBioHealth, Sungkyunkwan University, Suwon 16419, Republic of Korea"},{"name":"Department of Biopharmaceutical Convergence, Sungkyunkwan University, Suwon 16419, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7895-5112","authenticated-orcid":false,"given":"Gyudo","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioinformatics, Korea University, Sejong 30019, Republic of Korea"},{"name":"Interdisciplinary Graduate Program for Artificial Intelligence Smart Convergence Technology, Korea University, Sejong 30019, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1146\/annurev.neuro.26.010302.081142","article-title":"Protofibrils, Pores, Fibrils, and Neurodegeneration: Separating the Responsible Protein Aggregates from The Innocent Bystanders","volume":"26","author":"Caughey","year":"2003","journal-title":"Annu. 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