{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T23:20:53Z","timestamp":1788909653562,"version":"build-2803163510"},"reference-count":33,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,21]],"date-time":"2024-08-21T00:00:00Z","timestamp":1724198400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["19-73-30017-P"],"award-info":[{"award-number":["19-73-30017-P"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Brucellosis in animals is an infectious disease caused by bacteria of the genus Brucella. Known methods for diagnosing brucellosis face some challenges, due to the difficulties in isolating and standardizing the natural brucellosis antigen. In this work, we investigated the possibility of using the fluorescence polarization assay (FPA) with synthetic glycoconjugate biosensing tracers to detect antibodies against Brucella as a new methodology for diagnosing brucellosis. Based on the received results, the synthetic fluorescein-labeled trisaccharide tracer is most effective for Brucellosis detection. This tracer is structurally related to the immune determinant fragment of the Brucella LPS buildup of N-formyl-d-perosamine units, connected via \u03b1-(1\u21923)-linkage at the non-reducing end and \u03b1-(1\u21922)-linkage at the reducing end. The sensitivity and specificity in the case of the use of trisaccharide tracer 3b were 71% and 100% (Yuden\u2019s method) and 87% and 88% (Euclidean method), respectively, which is comparable with the diagnostic efficiency of traditionally used serological methods, such as the agglutination test (AT), complement fixation test (CFT), and Rose Bengal test (RBT). Given the known advantages of FPA (e.g., speed, compactness of the equipment, and standard reagents) and the increased specificity of the developed test system, it would be appropriate to consider its widespread use for the diagnosis of brucellosis in animals, including rapid testing in the field.<\/jats:p>","DOI":"10.3390\/bios14080404","type":"journal-article","created":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T06:28:51Z","timestamp":1724308131000},"page":"404","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Applying a Fluorescence Polarization Assay for Detection of Brucellosis in Animals Using the Fluorescently Labeled Synthetic Oligosaccharides as Biosensing Tracer"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5896-2523","authenticated-orcid":false,"given":"Liliya I.","family":"Mukhametova","sequence":"first","affiliation":[{"name":"Department of Chemistry, Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3749-7383","authenticated-orcid":false,"given":"Dmitry O.","family":"Zherdev","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6936-0720","authenticated-orcid":false,"given":"Sergei A.","family":"Eremin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3304-1557","authenticated-orcid":false,"given":"Anton N.","family":"Kuznetsov","sequence":"additional","affiliation":[{"name":"N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktor I.","family":"Yudin","sequence":"additional","affiliation":[{"name":"N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oleg D.","family":"Sclyarov","sequence":"additional","affiliation":[{"name":"Russian State Centre of Quality and Standardization of Veterinary Drugs and Feeds, 123022 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olesia V.","family":"Babicheva","sequence":"additional","affiliation":[{"name":"Russian State Centre of Quality and Standardization of Veterinary Drugs and Feeds, 123022 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3374-2601","authenticated-orcid":false,"given":"Anton V.","family":"Motorygin","sequence":"additional","affiliation":[{"name":"Russian State Centre of Quality and Standardization of Veterinary Drugs and Feeds, 123022 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yury E.","family":"Tsvetkov","sequence":"additional","affiliation":[{"name":"N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9477-4610","authenticated-orcid":false,"given":"Vadim B.","family":"Krylov","sequence":"additional","affiliation":[{"name":"N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0727-4050","authenticated-orcid":false,"given":"Nikolay E.","family":"Nifantiev","sequence":"additional","affiliation":[{"name":"N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1080\/01652176.2020.1868616","article-title":"Bovine Brucellosis\u2014A Comprehensive Review","volume":"41","author":"Khurana","year":"2021","journal-title":"Vet. Q."},{"key":"ref_2","first-page":"177","article-title":"Current Methods of Human and Animal Brucellosis Diagnostics","volume":"3","author":"Smirnova","year":"2013","journal-title":"Adv. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"881","DOI":"10.13005\/bpj\/1181","article-title":"Diagnosis of Cattle Brucellosis by PCR and Serological Methods: Comparison of Diagnostic Tests","volume":"14","author":"Saadat","year":"2017","journal-title":"Biomed. Pharmacol. 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