{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T17:19:02Z","timestamp":1781630342755,"version":"3.54.5"},"reference-count":30,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T00:00:00Z","timestamp":1724371200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NHMRC Investigator Grant","award":["GNT2025849"],"award-info":[{"award-number":["GNT2025849"]}]},{"name":"Lord Mayor\u2019s Charitable Foundation and the Ray William Houston Trust","award":["GNT2025849"],"award-info":[{"award-number":["GNT2025849"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Loop-mediated isothermal amplification (LAMP) has attracted significant attention for rapid and accurate point-of-care diagnostics. However, integrating sample introduction, lysis, amplification, and detection steps into an easy-to-use, disposable system has so far been challenging. This has limited the uptake of the technique in practical applications. In this study, we developed a colourimetric one-step LAMP assay that combines thermolysis and LAMP reaction, to detect the SARS-CoV-2 virus in nasopharyngeal swab samples from COVID-19-infected individuals. The limit of detection was 500 copies per reaction at 65 \u00b0C for 25 min in reaction tubes. Additionally, we developed a finger-operated capillary-driven microfluidic device with selective PVA coating. This finger-actuated microfluidic device could self-dose the required sample amount for the LAMP reaction and inhibit sample evaporation. Finally, we integrated the LAMP assay into the microfluidic device by short-term pre-storage of the LAMP master mix. Using this device, nasopharyngeal swab samples from COVID-19-infected individuals showed positive results at a reaction time of 35 min at 65 \u00b0C. This integrated device may be adapted to detect other RNA viruses of interest rapidly.<\/jats:p>","DOI":"10.3390\/bios14090410","type":"journal-article","created":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T12:53:19Z","timestamp":1724417599000},"page":"410","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A Finger-Actuated Sample-Dosing Capillary-Driven Microfluidic Device for Loop-Mediated Isothermal Amplification"],"prefix":"10.3390","volume":"14","author":[{"given":"Xuan","family":"Le","sequence":"first","affiliation":[{"name":"Department of Neuroscience, School of Translational Medicine, Monash University, Melbourne, VIC 3004, Australia"},{"name":"Dynamic Micro Devices Laboratory, Department of Mechanical and Aerospace Engineering, Monash University, Clayton, VIC 3800, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianxiong","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, School of Translational Medicine, Monash University, Melbourne, VIC 3004, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"McMahon","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease, Alfred Hospital and School of Translational Medicine, Monash University, Melbourne, VIC 3004, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica A.","family":"Wisniewski","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease, Alfred Hospital and School of Translational Medicine, Monash University, Melbourne, VIC 3004, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Coldham","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease, Alfred Hospital and School of Translational Medicine, Monash University, Melbourne, VIC 3004, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuncay","family":"Alan","sequence":"additional","affiliation":[{"name":"Dynamic Micro Devices Laboratory, Department of Mechanical and Aerospace Engineering, Monash University, Clayton, VIC 3800, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Kwan","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, School of Translational Medicine, Monash University, Melbourne, VIC 3004, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.ab.2015.11.019","article-title":"Isothermal amplification detection of nucleic acids by a double-nicked beacon","volume":"496","author":"Shi","year":"2016","journal-title":"Anal. Biochem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1111\/jam.13647","article-title":"Loop-mediated isothermal amplification (LAMP): A versatile technique for detection of micro-organisms","volume":"124","author":"Wong","year":"2018","journal-title":"J. Appl. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.micpath.2017.03.016","article-title":"Loop-mediated isothermal amplification (LAMP): A novel rapid detection platform for pathogens","volume":"107","author":"Li","year":"2017","journal-title":"Microb. Pathog."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1080\/14737159.2023.2169071","article-title":"Advances in RT-LAMP for COVID-19 testing and diagnosis","volume":"23","author":"Choi","year":"2023","journal-title":"Expert Rev. Mol. Diagn."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"eabc7075","DOI":"10.1126\/scitranslmed.abc7075","article-title":"A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples","volume":"12","author":"Herbst","year":"2020","journal-title":"Sci. Transl. Med."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Tan, S.C., and Yiap, B.C. (2009). DNA, RNA, and protein extraction: The past and the present. J. Biomed. Biotechnol., 2009.","DOI":"10.1155\/2009\/574398"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Tallmadge, R.L., Laverack, M., Cronk, B., Venugopalan, R., Martins, M., Zhang, X., Elvinger, F., Plocharczyk, E., and Diel, D.G. (2022). Viral RNA Load and Infectivity of SARS-CoV-2 in Paired Respiratory and Oral Specimens from Symptomatic, Asymptomatic, or Postsymptomatic Individuals. Microbiol. Spectr., 10.","DOI":"10.1128\/spectrum.02264-21"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Karino, M., Harada, M., Yamada, C., Fukuoka, K., Sugo, M., Hanada, H., Masuda, D., Adachi, S., Nakao, S., and Seki, M. (2021). Evaluation of the efficacy of LAMP-based SARS-CoV-2 detection with simple RNA extraction from nasopharyngeal swabs: A prospective observational study. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0260732"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Gao, D., Guo, X., Yang, Y., Shi, H., Hao, R., Wang, S., Li, Z.J., Zhao, R., and Song, H. (2022). Microfluidic chip and isothermal amplification technologies for the detection of pathogenic nucleic acid. J. Biol. Eng., 16.","DOI":"10.1186\/s13036-022-00312-w"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"11652","DOI":"10.1039\/D0RA00263A","article-title":"Advances in passively driven microfluidics and lab-on-chip devices: A comprehensive literature review and patent analysis","volume":"10","author":"Narayanamurthy","year":"2020","journal-title":"RSC Adv."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1039\/C7LC01128H","article-title":"Finger-actuated microfluidic device for the blood cross-matching test","volume":"18","author":"Park","year":"2018","journal-title":"Lab Chip"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2973","DOI":"10.1039\/C9LC00422J","article-title":"Integrated microfluidic pumps and valves operated by finger actuation","volume":"19","author":"Park","year":"2019","journal-title":"Lab Chip"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1039\/D0LC00047G","article-title":"Towards practical sample preparation in point-of-care testing: User-friendly microfluidic devices","volume":"20","author":"Park","year":"2020","journal-title":"Lab Chip"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2323","DOI":"10.1039\/C8LC00458G","article-title":"Capillary microfluidics in microchannels: From microfluidic networks to capillaric circuits","volume":"18","author":"Olanrewaju","year":"2018","journal-title":"Lab Chip"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1007\/s10404-007-0256-2","article-title":"Valves for autonomous capillary systems","volume":"5","author":"Zimmermann","year":"2008","journal-title":"Microfluid. Nanofluidics"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/S0925-4005(00)00675-4","article-title":"Hydrophobic valves of plasma deposited octafluorocyclobutane in DRIE channels","volume":"75","author":"Andersson","year":"2001","journal-title":"Sens. Actuators B Chem."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Bruijns, B., Folkertsma, L., and Tiggelaar, R. (2022). FDA authorized molecular point-of-care SARS-CoV-2 tests: A critical review on principles, systems and clinical performances. Biosens. Bioelectron. X, 11.","DOI":"10.1016\/j.biosx.2022.100158"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Deng, H., Jayawardena, A., Chan, J., Tan, S.M., Alan, T., and Kwan, P. (2021). An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-94652-0"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e144","DOI":"10.1093\/nar\/gks588","article-title":"Quantification of RNA integrity and its use for measurement of transcript number","volume":"40","author":"Brisco","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.bios.2018.03.009","article-title":"Fully 3D printed integrated reactor array for point-of-care molecular diagnostics","volume":"109","author":"Kadimisetty","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"7180","DOI":"10.1021\/acs.analchem.0c04944","article-title":"PDMS curing inhibition on 3D-printed molds: Why? Also, how to avoid it?","volume":"93","author":"Venzac","year":"2021","journal-title":"Anal. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"16091","DOI":"10.1038\/micronano.2016.91","article-title":"Hydrophilic surface modification of PDMS for droplet microfluidics using a simple, quick, and robust method via PVA deposition","volume":"3","author":"Trantidou","year":"2017","journal-title":"Microsyst. Nanoeng."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Huang, C., Wippold, J.A., Stratis-Cullum, D., and Han, A. (2020). Eliminating air bubble in microfluidic systems utilizing integrated in-line sloped microstructures. Biomed. Microdevices, 22.","DOI":"10.1007\/s10544-020-00529-w"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"6139","DOI":"10.1021\/ac0261449","article-title":"Autonomous microfluidic capillary system","volume":"74","author":"Juncker","year":"2002","journal-title":"Anal. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1002\/elps.200406157","article-title":"Multilayer poly(vinyl alcohol)-adsorbed coating on poly(dimethylsiloxane) microfluidic chips for biopolymer separation","volume":"26","author":"Wu","year":"2005","journal-title":"Electrophoresis"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1007\/s10544-005-6070-2","article-title":"Characterization of polydimethylsiloxane (PDMS) properties for biomedical micro\/nanosystems","volume":"7","author":"Mata","year":"2005","journal-title":"Biomed. Microdevices"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"30754","DOI":"10.1039\/D2RA05901K","article-title":"Influence of reactive oxygen species concentration and ambient temperature on the evolution of chemical bonds during plasma cleaning: A molecular dynamics simulation","volume":"12","author":"Li","year":"2022","journal-title":"RSC Adv."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"8377","DOI":"10.1039\/C4RA12771D","article-title":"Low temperature and deformation-free bonding of PMMA microfluidic devices with stable hydrophilicity via oxygen plasma treatment and PVA coating","volume":"5","author":"Yu","year":"2015","journal-title":"RSC Adv."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Lin, P.-H., and Li, B.-R. (2021). Passively driven microfluidic device with simple operation in the development of nanolitre droplet assay in nucleic acid detection. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-00470-9"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"106670","DOI":"10.1016\/j.polymertesting.2020.106670","article-title":"Experimental study on mechanical performance of polydimethylsiloxane (PDMS) at various temperatures","volume":"90","author":"Zhang","year":"2020","journal-title":"Polym. Test."}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/14\/9\/410\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:42:19Z","timestamp":1760110939000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/14\/9\/410"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,23]]},"references-count":30,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["bios14090410"],"URL":"https:\/\/doi.org\/10.3390\/bios14090410","relation":{},"ISSN":["2079-6374"],"issn-type":[{"value":"2079-6374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,23]]}}}