{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T22:53:08Z","timestamp":1793055188795,"version":"4.5.0"},"reference-count":45,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T00:00:00Z","timestamp":1730678400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"UPES University","award":["UPES\/R&D-SEED-INFRA\/11032022\/01"],"award-info":[{"award-number":["UPES\/R&D-SEED-INFRA\/11032022\/01"]}]},{"name":"UPES SEED","award":["UPES\/R&D-SEED-INFRA\/11032022\/01"],"award-info":[{"award-number":["UPES\/R&D-SEED-INFRA\/11032022\/01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Prostate cancer is one of the most prevalent cancers afflicting men worldwide, often detected at advanced stages, leading to increased mortality rates. Addressing this challenge, we present an innovative approach employing electrochemical biosensing for early-stage prostate cancer detection. This study used Indium\u2013Tin Oxide (ITO) as a substrate and a deposited gold\u2013graphene quantum dot (Au\u2013GQD) nanohybrid to establish electrochemical sensing platforms for DNA-hybridization assays. A capturing DNA probe, PCA3, was covalently immobilized on the surface of the Au\u2013GQDs and deposited electrochemically onto the ITO electrode surface. The Au\u2013GQDs enabled the capturing of the target PCA3 biomarker probe. The sensor achieved a limit of detection (LoD) of up to 211 fM and presented a linear detection range spanning 1 \u00b5M to 100 fM. A rapid 5-min response time was also achieved. The tested shelf life of the pre-immobilized sensor was approximately 19 \u00b1 1 days, with pronounced selectivity for its intended target amidst various interferants. The sensing device has the potential to revolutionize prostate cancer management by facilitating early-stage detection and screening with enhanced treatment efficacy.<\/jats:p>","DOI":"10.3390\/bios14110534","type":"journal-article","created":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T10:57:20Z","timestamp":1730717840000},"page":"534","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Gold\u2013Graphene Quantum Dot Hybrid Nanoparticle for Smart Diagnostics of Prostate Cancer"],"prefix":"10.3390","volume":"14","author":[{"given":"Divakar","family":"Raj","sequence":"first","affiliation":[{"name":"Allied Health Sciences, School of Health Sciences and Technology, University of Petroleum and Energy Studies, Dehradun 248007, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8946-5909","authenticated-orcid":false,"given":"Arun","family":"Kumar","sequence":"additional","affiliation":[{"name":"Mahavir Cancer Sansthan, and Research Centre, Patna 801505, Bihar, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhruv","family":"Kumar","sequence":"additional","affiliation":[{"name":"Allied Health Sciences, School of Health Sciences and Technology, University of Petroleum and Energy Studies, Dehradun 248007, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2948-9471","authenticated-orcid":false,"given":"Krishna","family":"Kant","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Engineering and Applied Sciences, Bennett University, Greater Noida 201310, Uttar Pradesh, India"},{"name":"CINBIO, Universidade de Vigo, Campus Universitario Lagoas Marcosende, 36310 Vigo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5365-5807","authenticated-orcid":false,"given":"Ashish","family":"Mathur","sequence":"additional","affiliation":[{"name":"Centre for Interdisciplinary Research and Innovation (CIDRI), University of Petroleum and Energy Studies, Dehradun 248007, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1932","DOI":"10.1016\/j.bios.2006.01.006","article-title":"Point-of-Care Biosensor Systems for Cancer Diagnostics\/Prognostics","volume":"21","author":"Soper","year":"2006","journal-title":"Biosens. Bioelectron."},{"key":"ref_2","unstructured":"(2024, August 11). Cancer Tomorrow. Available online: https:\/\/gco.iarc.fr\/tomorrow\/en."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"63","DOI":"10.14740\/wjon1191","article-title":"Epidemiology of Prostate Cancer","volume":"10","author":"Rawla","year":"2019","journal-title":"World J. Oncol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Oliveira, M., Concei\u00e7\u00e3o, P., Kant, K., Ainla, A., and Di\u00e9guez, L. (2021). Electrochemical Sensing in 3D Cell Culture Models: New Tools for Developing Better Cancer Diagnostics and Treatments. Cancers, 13.","DOI":"10.3390\/cancers13061381"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"46645","DOI":"10.1021\/acsami.9b19180","article-title":"Detection of the Prostate Cancer Biomarker PCA3 with Electrochemical and Impedance-Based Biosensors","volume":"11","author":"Soares","year":"2019","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1515\/cclm-2012-0392","article-title":"Prostate Cancer Antigen 3 (PCA3) RNA Detection in Blood and Tissue Samples for Prostate Cancer Diagnosis","volume":"51","author":"Neves","year":"2013","journal-title":"Clin. Chem. Lab. Med. (CCLM)"},{"key":"ref_7","first-page":"1686991","article-title":"Urinary PCA3 a Superior Diagnostic Biomarker for Prostate Cancer among Ghanaian Men","volume":"2022","author":"Fondjo","year":"2022","journal-title":"Dis. Markers"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Pasinszki, T., Krebsz, M., Tung, T.T., and Losic, D. (2017). Carbon Nanomaterial Based Biosensors for Non-Invasive Detection of Cancer and Disease Biomarkers for Clinical Diagnosis. Sensors, 17.","DOI":"10.3390\/s17081919"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"523","DOI":"10.7150\/jca.13821","article-title":"Immunochemical Assays and Nucleic-Acid Detection Techniques for Clinical Diagnosis of Prostate Cancer","volume":"7","author":"Kanyong","year":"2016","journal-title":"J. Cancer"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Nabok, A., Abu-Ali, H., Takita, S., and Smith, D.P. (2021). Electrochemical Detection of Prostate Cancer Biomarker PCA3 Using Specific RNA-Based Aptamer Labelled with Ferrocene. Chemosensors, 9.","DOI":"10.3390\/chemosensors9040059"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5895","DOI":"10.2174\/0929867329666220607162250","article-title":"Detection of Prostate Cancer Biomarker PCA3 by Using Aptasensors","volume":"29","author":"Crulhas","year":"2022","journal-title":"Curr. Med. Chem."},{"key":"ref_12","unstructured":"(2024, September 20). Available online: https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC7214775."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Menaa, F., Fatemeh, Y., Vashist, S.K., Iqbal, H., Sharts, O.N., and Menaa, B. (2021). Graphene, an Interesting Nanocarbon Allotrope for Biosensing Applications: Advances, Insights, and Prospects. Biomed. Eng. Comput. Biol., 12.","DOI":"10.1177\/1179597220983821"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Pandey, R.R., and Chusuei, C.C. (2021). Carbon Nanotubes, Graphene, and Carbon Dots as Electrochemical Biosensing Composites. Molecules, 26.","DOI":"10.3390\/molecules26216674"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Mansuriya, B.D., and Altintas, Z. (2020). Graphene Quantum Dot-Based Electrochemical Immunosensors for Biomedical Applications. Materials, 13.","DOI":"10.3390\/ma13010096"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.1002\/elan.201500490","article-title":"A Sensitive and Selective Electrochemical Sensor Based on Graphene Quantum Dot\/Gold Nanoparticle Nanocomposite Modified Electrode for the Determination of Quercetin in Biological Samples","volume":"28","author":"Li","year":"2016","journal-title":"Electroanalysis"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.micromeso.2011.12.011","article-title":"Gold Nanotube Membranes Have Catalytic Properties","volume":"153","author":"Yu","year":"2012","journal-title":"Microporous Mesoporous Mater."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"146427","DOI":"10.1016\/j.apsusc.2020.146427","article-title":"Graphene Quantum Dot-Gold Hybrid Nanoparticles Integrated Aptasensor for Ultra-Sensitive Detection of Vitamin D3 towards Point-of-Care Application","volume":"521","author":"Wadhwa","year":"2020","journal-title":"Appl. Surf. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3447","DOI":"10.1021\/am100502u","article-title":"Nanopore Gradients on Porous Aluminum Oxide Generated by Nonuniform Anodization of Aluminum","volume":"2","author":"Kant","year":"2010","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Sanko, V., and Kuralay, F. (2023). Label-Free Electrochemical Biosensor Platforms for Cancer Diagnosis: Recent Achievements and Challenges. Biosensors, 13.","DOI":"10.3390\/bios13030333"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1887","DOI":"10.1016\/j.bios.2005.10.027","article-title":"Electrochemical Biosensors: Towards Point-of-Care Cancer Diagnostics","volume":"21","author":"Wang","year":"2006","journal-title":"Biosens. Bioelectron."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"101310","DOI":"10.1016\/j.surfin.2021.101310","article-title":"Design and Development of a Novel Flexible Molecularly Imprinted Electroanalytical Sensor for the Monitoring of Diabetic Foot Ulcers","volume":"26","author":"Roy","year":"2021","journal-title":"Surf. Interfaces"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"100963","DOI":"10.1016\/j.mex.2020.100963","article-title":"Engineering of Luminescent Graphene Quantum Dot-Gold (GQD-Au) Hybrid Nanoparticles for Functional Applications","volume":"7","author":"Wadhwa","year":"2020","journal-title":"MethodsX"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"116413","DOI":"10.1016\/j.trac.2021.116413","article-title":"In-Situ Redox-Active Hybrid Graphene Platform for Label-Free Electrochemical Biosensor: Insights from Electrodeposition and Electroless Deposition","volume":"143","author":"Kanagavalli","year":"2021","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.jelechem.2011.10.014","article-title":"Gold Nanoparticles Electrodeposited on Glassy Carbon Using Cyclic Voltammetry: Application to Hg(II) Trace Analysis","volume":"664","author":"Hezard","year":"2012","journal-title":"J. Electroanal. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1016\/j.msec.2016.07.026","article-title":"Graphene Quantum Dots Decorated with Magnetic Nanoparticles: Synthesis, Electrodeposition, Characterization and Application as an Electrochemical Sensor towards Determination of Some Amino Acids at Physiological PH","volume":"68","author":"Hasanzadeh","year":"2016","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Zhao, G., and Liu, G. (2018). Electrochemical Deposition of Gold Nanoparticles on Reduced Graphene Oxide by Fast Scan Cyclic Voltammetry for the Sensitive Determination of As(III). Nanomaterials, 9.","DOI":"10.3390\/nano9010041"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5303","DOI":"10.1039\/C5AY01062D","article-title":"Single-Walled Carbon Nanotubes\u2013Carboxyl-Functionalized Graphene Oxide-Based Electrochemical DNA Biosensor for Thermolabile Hemolysin Gene Detection","volume":"7","author":"Yang","year":"2015","journal-title":"Anal. Methods"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.mtchem.2018.09.007","article-title":"Graphene Quantum Dots from Chemistry to Applications","volume":"10","author":"Tian","year":"2018","journal-title":"Mater. Today Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2853","DOI":"10.1016\/j.arabjc.2015.06.020","article-title":"Synthesis, Structural and Optical Properties of Gold Nanoparticle-Graphene-Selenocysteine Composite Bismuth Ultrathin Film Electrode and Its Application to Pb(II) and Cd(II) Determination","volume":"12","author":"Ibrahim","year":"2019","journal-title":"Arab. J. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"6318","DOI":"10.1039\/C5CC00061K","article-title":"Selective Oxidation of Veratryl Alcohol with Composites of Au Nanoparticles and Graphene Quantum Dots as Catalysts","volume":"51","author":"Wu","year":"2015","journal-title":"Chem. Commun."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"211016","DOI":"10.20964\/2021.10.15","article-title":"Au-Ag Nanoparticles-Graphene Quantum Dots as Sensor for Highly Sensitive Electrochemical Determination of Insulin Level in Pharmaceutical Samples","volume":"16","author":"Wang","year":"2021","journal-title":"Int. J. Electrochem. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"28550","DOI":"10.1021\/acsami.0c06753","article-title":"Microplasma-Enabled Graphene Quantum Dots Wrapped Gold Nanoparticles Photodetection with Synergistic Enhancement for Broadband","volume":"12","author":"Thakur","year":"2020","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1007\/s00604-019-3701-5","article-title":"Electrochemical Aflatoxin B1 Immunosensor Based on the Use of Graphene Quantum Dots and Gold Nanoparticles","volume":"186","author":"Bhardwaj","year":"2019","journal-title":"Microchim. Acta"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5406","DOI":"10.1039\/C4RA12713G","article-title":"Protein Conjugated Carboxylated Gold@reduced Graphene Oxide for Aflatoxin B 1 Detection","volume":"5","author":"Srivastava","year":"2015","journal-title":"RSC Adv."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"6116","DOI":"10.1002\/chem.200900596","article-title":"Controlled Synthesis of Large-Area and Patterned Electrochemically Reduced Graphene Oxide Films","volume":"15","author":"Zhou","year":"2009","journal-title":"Chemistry"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Singhal, C., Khanuja, M., Chaudhary, N., Pundir, C.S., and Narang, J. (2018). Detection of Chikungunya Virus DNA Using Two-Dimensional MoS2 Nanosheets Based Disposable Biosensor. Sci. Rep., 8.","DOI":"10.1038\/s41598-018-25824-8"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Krishnaveni, P., and Ganesh, V. (2021). Electron Transfer Studies of a Conventional Redox Probe in Human Sweat and Saliva Bio-Mimicking Conditions. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-86866-z"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1016\/j.talanta.2008.12.053","article-title":"An Electrochemical Biosensor for Detection of PML\/RARA Fusion Gene Using Capture Probe Covalently Immobilized onto Poly-Calcon Carboxylic Acid Modified Glassy Carbon Electrode","volume":"78","author":"Wei","year":"2009","journal-title":"Talanta"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1007\/s00604-014-1195-8","article-title":"Impedimetric Aptasensor for Staphylococcus Aureus Based on Nanocomposite Prepared from Reduced Graphene Oxide and Gold Nanoparticles","volume":"181","author":"Jia","year":"2014","journal-title":"Microchim. Acta"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e10122","DOI":"10.1002\/mds3.10122","article-title":"Detection of Prostate Cancer DNA Using Tetrapods Based Disposable Paper Ecofriendly Biosensor Device","volume":"3","author":"Gautam","year":"2020","journal-title":"Med. Devices Sens."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Takita, S., Nabok, A., Lishchuk, A., Mussa, M.H., and Smith, D. (2022). Detection of Prostate Cancer Biomarker PCA3 with Electrochemical Apta-Sensor. Eng. Proc., 16.","DOI":"10.3390\/IECB2022-12257"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez-Salcedo, R., Miranda-Castro, R., De-los-Santos-\u00c1lvarez, N., and Lobo-Casta\u00f1\u00f3n, M.J. (2021). Dual Electrochemical Genosensor for Early Diagnosis of Prostate Cancer through LncRNAs Detection. Biosens. Bioelectron., 192.","DOI":"10.1016\/j.bios.2021.113520"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.jtice.2021.03.004","article-title":"Lectin Bioreceptor Approach in Capacitive Biosensor for Prostate-Specific Membrane Antigen Detection in Diagnosing Prostate Cancer","volume":"120","author":"Subramani","year":"2021","journal-title":"J. Taiwan Inst. Chem. Eng."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.bios.2018.09.003","article-title":"Construction and Application of Amperometric Sarcosine Biosensor Based on SOxNPs\/AuE for Determination of Prostate Cancer","volume":"122","author":"Kumar","year":"2018","journal-title":"Biosens. Bioelectron."}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/14\/11\/534\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:28:23Z","timestamp":1760113703000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/14\/11\/534"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,4]]},"references-count":45,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2024,11]]}},"alternative-id":["bios14110534"],"URL":"https:\/\/doi.org\/10.3390\/bios14110534","relation":{},"ISSN":["2079-6374"],"issn-type":[{"value":"2079-6374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,4]]}}}