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This condition is typically asymptomatic until the second or even third trimester, which can lead to poor outcomes and can be costly. Detection is particularly challenging in low- and middle-income countries, where a lack of centralized testing facilities coincides with high rates of PE-related maternal mortality. Variations in the levels of hsa-miR-17-5p have been identified as constituting a potential early indicator for distinguishing between individuals with PE and those without PE during the first trimester. Thus, developing a screening test to measure hsa-miR-17-5p levels would not only facilitate rapid detection in the early stages of pregnancy but also help democratize testing globally. Here, we present a proof-of-principle lateral-flow assay (LFA) designed to measure hsa-miR-17-5p levels using DNA-hairpin recognition elements for enhanced specificity and nanoprobes for sensitive surface-enhanced resonance Raman scattering (SERS) signal transduction. The theoretical limit of detection for hsa-miR-17-5p was 3.84 \u00d7 10\u22124 pg\/\u00b5L using SERS.<\/jats:p>","DOI":"10.3390\/bios14110535","type":"journal-article","created":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T06:30:48Z","timestamp":1730788248000},"page":"535","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Lateral Flow Assay for Preeclampsia Screening Using DNA Hairpins and Surface-Enhanced Raman-Active Nanoprobes Targeting hsa-miR-17-5p"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5452-529X","authenticated-orcid":false,"given":"Ka Wai","family":"Ng","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, Texas A&M University, 5045 Emerging Technologies Building, College Station, TX 77843, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siddhant","family":"Jaitpal","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Texas A&M University, 5045 Emerging Technologies Building, College Station, TX 77843, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ngoc Nhu","family":"Vu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Texas A&M University, 5045 Emerging Technologies Building, College Station, TX 77843, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela Michelle T.","family":"San Juan","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Texas A&M University, 5045 Emerging Technologies Building, College Station, TX 77843, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sayantan","family":"Tripathy","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Texas A&M University, 5045 Emerging Technologies Building, College Station, TX 77843, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7096-6335","authenticated-orcid":false,"given":"Rohit Sai","family":"Kodam","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Texas A&M University, 5045 Emerging Technologies Building, College Station, TX 77843, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abhishek","family":"Bastiray","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae-Hyun","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahua","family":"Choudhury","sequence":"additional","affiliation":[{"name":"Irma Lerma Rangel College of Pharmacy, Texas A&M University, 159 Reynolds Medical Building, College Station, TX 77843, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3164-9625","authenticated-orcid":false,"given":"Gerard L.","family":"Cot\u00e9","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Texas A&M University, 5045 Emerging Technologies Building, College Station, TX 77843, USA"},{"name":"Center for Remote Health Technologies & Systems, Texas A&M Engineering Experimentation Station, 1041 Emerging Technologies Building, College Station, TX 77843, USA"},{"name":"Department of Electrical Engineering, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4926-5467","authenticated-orcid":false,"given":"Samuel","family":"Mabbott","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Texas A&M University, 5045 Emerging Technologies Building, College Station, TX 77843, USA"},{"name":"Center for Remote Health Technologies & Systems, Texas A&M Engineering Experimentation Station, 1041 Emerging Technologies Building, College Station, TX 77843, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,5]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"ACOG Practice Bulletin No. 202: Gestational Hypertension and Preeclampsia","volume":"133","author":"Espinoza","year":"2019","journal-title":"Obstet. 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