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Early diagnosis is crucial for mitigating its adverse effects, and recent investigations have identified endoglin as a potential biomarker for this purpose. Here, we present the development of a hybrid biosensor platform for the ultrasensitive detection of endoglin, aimed at enabling the early diagnosis of preeclampsia. This platform integrates the high surface area properties of nanoporous anodic alumina (NAA) with the unique optical characteristics of gold nanoclusters (AuNCs) to achieve enhanced detection capabilities. The NAA surface functionalized to promote attachment of AuNCs, which then was functionalized with specific antibodies to confer selectivity towards endoglin. Photoluminescence (PL) analysis of the biosensor demonstrated a linear detection range of 10\u201350 ng\/mL, with a detection limit of 5.4 ng\/mL and a sensitivity of 0.004 a.u.\/(ng\/mL). This proof-of-concept study suggests that the NAA-AuNCs-based biosensing platform holds significant potential for the development of ultrasensitive, portable, and cost-effective diagnostic tools for preeclampsia, offering a promising avenue for advancing prenatal care.<\/jats:p>","DOI":"10.3390\/bios14120610","type":"journal-article","created":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T08:10:47Z","timestamp":1734077447000},"page":"610","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Sensing Platform Based on Gold Nanoclusters and Nanoporous Anodic Alumina for Preeclampsia Detection"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-6067-8246","authenticated-orcid":false,"given":"Josep Maria","family":"Cantons","sequence":"first","affiliation":[{"name":"Department of Electronics, Electric, and Automatic Engineering, Rovira I Virgili University (URV), 43007 Tarragona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1253-8126","authenticated-orcid":false,"given":"Akash","family":"Bachhuka","sequence":"additional","affiliation":[{"name":"Department of Electronics, Electric, and Automatic Engineering, Rovira I Virgili University (URV), 43007 Tarragona, Spain"},{"name":"Institute of Chemical Research of Catalonia (ICIQ), 43007 Tarragona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5976-1408","authenticated-orcid":false,"given":"Lluis F.","family":"Marsal","sequence":"additional","affiliation":[{"name":"Department of Electronics, Electric, and Automatic Engineering, Rovira I Virgili University (URV), 43007 Tarragona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Hansson, T., Andersson, M.E., Ahlstr\u00f6m, G., and Hansson, S.R. (2022). Women\u2019s experiences of preeclampsia as a condition of uncertainty: A qualitative study. BMC Pregnancy Childbirth, 22.","DOI":"10.1186\/s12884-022-04826-5"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/S0140-6736(15)00070-7","article-title":"Pre-eclampsia","volume":"387","author":"Mol","year":"2016","journal-title":"Lancet"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1038\/s41419-019-2162-4","article-title":"Pyroptosis is a critical inflammatory pathway in the placenta from early onset preeclampsia and in human trophoblasts exposed to hypoxia and endoplasmic reticulum stressors","volume":"10","author":"Cheng","year":"2019","journal-title":"Cell Death Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1093\/humupd\/dms060","article-title":"Genetic variants in pre-eclampsia: A meta-analysis","volume":"19","author":"Buurma","year":"2013","journal-title":"Hum. Reprod. Updat."},{"key":"ref_5","first-page":"1009","article-title":"Ultrasound markers for the detection of women at risk of developing pre-eclampsia","volume":"50","author":"Cynober","year":"2012","journal-title":"Clin. Chem. Lab. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1161\/HYPERTENSIONAHA.113.02293","article-title":"Angiogenic Factors in Diagnosis, Management, and Research in Preeclampsia","volume":"63","author":"Rana","year":"2014","journal-title":"Hypertension"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1002\/pd.2660","article-title":"Prediction of early, intermediate and late pre-eclampsia from maternal factors, biophysical and biochemical markers at 11\u201313 weeks","volume":"31","author":"Akolekar","year":"2011","journal-title":"Prenat. Diagn."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1161\/01.HYP.0000188408.49896.c5","article-title":"Preeclampsia: Recent insights","volume":"46","author":"Roberts","year":"2005","journal-title":"Hypertension"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.preghy.2012.01.001","article-title":"The placenta in preeclampsia","volume":"2","author":"Roberts","year":"2012","journal-title":"Pregnancy Hypertens."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e323","DOI":"10.1016\/S2214-109X(14)70227-X","article-title":"Global causes of maternal death: A WHO systematic analysis","volume":"2","author":"Say","year":"2014","journal-title":"Lancet Glob. Health"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ejogrb.2013.05.005","article-title":"Global and regional estimates of preeclampsia and eclampsia: A systematic review","volume":"170","author":"Abalos","year":"2013","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1097\/01.NPR.0000554088.97825.ad","article-title":"Preeclampsia and future stroke risk in women: What NPs need to know","volume":"44","author":"Davis","year":"2019","journal-title":"Nurse Pract."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"ACOG Committee on Obstetric Practice (2002). ACOG practice bulletin. Diagnosis and management of preeclampsia and eclampsia. Number 33, January 2002. American College of Obstetricians and Gynecologists. Int. J. Gynaecol. Obstet., 77, 67\u201375.","DOI":"10.1016\/S0029-7844(01)01747-1"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1055\/s-0039-1701023","article-title":"Preeclampsia: The Need for a Biological Definition and Diagnosis","volume":"38","author":"Vidaeff","year":"2021","journal-title":"Am. J. Perinatol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1039\/D3AN00213F","article-title":"A dichromatic plasmonic ELISA CD81 protein sensor for ultrasensitive detection of preeclampsia","volume":"148","author":"Chen","year":"2023","journal-title":"Analyst"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Kang, Y.-E., Seong, K.-Y., Yim, S.-G., Lee, Y., An, S.-M., Kim, S.C., Kim, K., An, B.-S., Lee, K.-S., and Yang, S.Y. (2020). Nanochannel-driven rapid capture of sub-nanogram level biomarkers for painless preeclampsia diagnosis. Biosens. Bioelectron., 163.","DOI":"10.1016\/j.bios.2020.112281"},{"key":"ref_17","first-page":"199","article-title":"The Use of Ultrasound and other Markers for Early Detection of Preeclampsia","volume":"12","author":"Nicolaides","year":"2016","journal-title":"Women\u2019s Health"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"266.e1","DOI":"10.1016\/j.ajog.2008.06.069","article-title":"First-trimester serum levels of soluble endoglin and soluble fms-like tyrosine kinase-1 as first-trimester markers for late-onset preeclampsia","volume":"199","author":"Baumann","year":"2008","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1038\/nm1429","article-title":"Soluble endoglin contributes to the pathogenesis of preeclampsia","volume":"12","author":"Venkatesha","year":"2006","journal-title":"Nat. Med."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Rana, S., Cerdeira, A.S., Wenger, J., Salahuddin, S., Lim, K.-H., Ralston, S.J., Thadhani, R.I., and Karumanchi, S.A. (2012). Plasma concentrations of soluble endoglin versus standard evaluation in patients with suspected preeclampsia. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0048259"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Andersson-Rusch, C., Liu, B., Quist-L\u00f8kken, I., Upton, P.D., Olsen, O.E., Hella, H., Yang, X., Tong, Z., Morrell, N.W., and Holien, T. (2023). High concentrations of soluble endoglin can inhibit BMP9 signaling in non-endothelial cells. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-33352-3"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1007\/s11010-021-04294-z","article-title":"The role of endoglin and its soluble form in pathogenesis of preeclampsia","volume":"477","author":"Petousis","year":"2022","journal-title":"Mol. Cell. Biochem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1002\/wnan.1245","article-title":"Gold nanoclusters with enhanced tunable fluorescence as bioimaging probes","volume":"6","author":"Palmal","year":"2014","journal-title":"WIREs Nanomed. Nanobiotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"8766","DOI":"10.1039\/D0CC03498C","article-title":"Tuning the properties of atomically precise gold nanoclusters for biolabeling and drug delivery","volume":"56","author":"Xu","year":"2020","journal-title":"Chem. Commun."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1021\/acs.bioconjchem.1c00475","article-title":"Gold Nanoclusters: Imaging, Therapy, and Theranostic Roles in Biomedical Applications","volume":"33","author":"Hebels","year":"2022","journal-title":"Bioconjugate Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2005499","DOI":"10.1002\/smll.202005499","article-title":"Gold nanoclusters: Atomically precise gold nanoclusters: Towards an optimal biocompatible system from a Theoretical\u2013Experimental Strategy","volume":"17","author":"Matus","year":"2021","journal-title":"Small"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"352001","DOI":"10.1088\/1361-6528\/ab2088","article-title":"Biocompatible gold nanoclusters: Synthetic strategies and biomedical prospects","volume":"30","author":"Zuber","year":"2019","journal-title":"Nanotechnology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"16662","DOI":"10.1021\/ja306199p","article-title":"From Aggregation-Induced Emission of Au(I)\u2013Thiolate Complexes to Ultrabright Au(0)@Au(I)\u2013Thiolate Core\u2013Shell Nanoclusters","volume":"134","author":"Luo","year":"2012","journal-title":"J. Am. Chem. Soc."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1007\/s10008-020-04683-2","article-title":"Nanoporous anodic alumina (NAA) prepared in different electrolytes with different pore sizes for humidity sensing","volume":"24","author":"Mir","year":"2020","journal-title":"J. Solid State Electrochem."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Choudhari, K.S., Choi, C.-H., Chidangil, S., and George, S.D. (2022). Recent Progress in the Fabrication and Optical Properties of Nanoporous Anodic Alumina. Nanomaterials, 12.","DOI":"10.3390\/nano12030444"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"042001","DOI":"10.1088\/0957-4484\/26\/4\/042001","article-title":"Low-cost fabrication technologies for nanostructures: State-of-the-art and potential","volume":"26","author":"Santos","year":"2015","journal-title":"Nanotechnology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.1021\/acsabm.9b01125","article-title":"Modulation of macrophages differentiation by Nanoscale-Engineered geometric and chemical features","volume":"3","author":"Bachhuka","year":"2020","journal-title":"ACS Appl. Bio Mater."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1007\/s10856-020-06398-2","article-title":"Nano-porous anodic alumina: Fundamentals and applications in tissue engineering","volume":"31","author":"Davoodi","year":"2020","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/978-3-319-20334-8_6","article-title":"Optical Properties of Nanoporous Anodic Alumina and Derived Applications","volume":"Volume 219","author":"Losic","year":"2015","journal-title":"Nanoporous Alumina. Springer Series in Materials Science"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Jani, A., Yazid, H., Habiballah, A., Mahmud, A., and Losic, D. (2015). Soft and Hard Surface Manipulation of Nanoporous Anodic Aluminum Oxide (AAO). Nanoporous Alumina, Springer.","DOI":"10.1007\/978-3-319-20334-8_5"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Domagalski, J.T., Xifre-Perez, E., and Marsal, L.F. (2021). Recent Advances in Nanoporous Anodic Alumina: Principles, Engineering, and Applications. Nanomaterials, 11.","DOI":"10.3390\/nano11020430"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"8093","DOI":"10.1021\/am4020814","article-title":"Gold-Coated Ordered Nanoporous Anodic Alumina Bilayers for Future Label-Free Interferometric Biosensors","volume":"5","author":"Macias","year":"2013","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1837","DOI":"10.1021\/ac500069f","article-title":"Structural and Optical Nanoengineering of Nanoporous Anodic Alumina Rugate Filters for Real-Time and Label-Free Biosensing Applications","volume":"86","author":"Kumeria","year":"2014","journal-title":"Anal. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"11878","DOI":"10.3390\/s140711878","article-title":"Nanoporous Anodic Alumina Platforms: Engineered Surface Chemistry and Structure for Optical Sensing Applications","volume":"14","author":"Kumeria","year":"2014","journal-title":"Sensors"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"12115","DOI":"10.1021\/acsanm.0c02615","article-title":"Enhancing forbidden light propagation in nanoporous anodic alumina Gradient-index filters by alcohol additives","volume":"3","author":"Lim","year":"2020","journal-title":"ACS Appl. Nano Mater."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"127302","DOI":"10.1016\/j.snb.2019.127302","article-title":"Remote sensing of Salmonella-specific DNA fragment by using nanoporous alumina modified with the single-strand DNA probe","volume":"304","author":"Tabrizi","year":"2020","journal-title":"Sens. Actuators B Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1021\/acssensors.0c02222","article-title":"Nanoporous Anodic Alumina-Based Sensor for miR-99a-5p Detection as an Effective Early Breast Cancer Diagnostic Tool","volume":"6","author":"Pla","year":"2021","journal-title":"ACS Sens."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Pol, L., Eckstein, C., Acosta, L.K., Xifr\u00e9-P\u00e9rez, E., Ferr\u00e9-Borrull, J., and Marsal, L.F. (2019). Real-Time Monitoring of Biotinylated Molecules Detection Dynamics in Nanoporous Anodic Alumina for Bio-Sensing. Nanomaterials, 9.","DOI":"10.3390\/nano9030478"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.snb.2017.10.156","article-title":"A label-free optical biosensor based on nanoporous anodic alumina for tumour necrosis factor-alpha detection in chronic wounds","volume":"257","author":"Rajeev","year":"2018","journal-title":"Sens. Actuators B Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1002\/pssa.201228150","article-title":"Understanding and morphology control of pore modulations in nanoporous anodic alumina by discontinuous anodization","volume":"209","author":"Santos","year":"2012","journal-title":"Phys. Status Solidi (a)"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1002\/pssa.201026435","article-title":"Hierarchical nanoporous anodic alumina templates by asymmetric two-step anodization","volume":"208","author":"Santos","year":"2010","journal-title":"Phys. Status Solidi (a)"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"4896","DOI":"10.1039\/b711200a","article-title":"Aminopropyltriethoxysilane (APTES)-functionalized nanoporous polymeric gratings: Fabrication and application in biosensing","volume":"17","author":"Hsiao","year":"2007","journal-title":"J. Mater. Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"7933","DOI":"10.1002\/anie.201002504","article-title":"Dressing in Layers: Layering Surface Functionalities in Nanoporous Aluminum Oxide Membranes","volume":"49","author":"Jani","year":"2010","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"429","DOI":"10.4103\/1947-2714.100998","article-title":"Western blot: Technique, theory, and trouble shooting","volume":"4","author":"Mahmood","year":"2012","journal-title":"N. Am. J. Med. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.jcis.2009.09.007","article-title":"Efficiency of blocking of non-specific interaction of different proteins by BSA adsorbed on hydrophobic and hydrophilic surfaces","volume":"341","author":"Jeyachandran","year":"2010","journal-title":"J. Colloid Interface Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.jim.2012.06.009","article-title":"Enzyme-linked immunosorbent assay (ELISA) and blocking with bovine serum albumin (BSA)\u2014Not all BSAs are alike","volume":"384","author":"Xiao","year":"2012","journal-title":"J. Immunol. Methods"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.trac.2014.02.011","article-title":"Fluorescent metal nanoclusters: From synthesis to applications","volume":"58","author":"Li","year":"2014","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"784097","DOI":"10.1155\/2015\/784097","article-title":"Fluorescent Gold Nanoclusters: Synthesis and Recent Biological Application","volume":"2015","author":"Qu","year":"2015","journal-title":"J. Nanomater."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/s10971-008-1800-6","article-title":"Hydrogen peroxide induced formation of peroxystannate nanoparticles","volume":"50","author":"Sladkevich","year":"2009","journal-title":"J. Sol-Gel Sci. Technol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2957","DOI":"10.3390\/molecules19032957","article-title":"The Influence of Chemical Surface Modification of Kenaf Fiber using Hydrogen Peroxide on the Mechanical Properties of Biodegradable Kenaf Fiber\/Poly(Lactic Acid) Composites","volume":"19","author":"Razak","year":"2014","journal-title":"Molecules"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s10934-017-0419-9","article-title":"Morphological and chemical characterization of highly ordered conical-pore anodic alumina prepared by multistep citric acid anodizing and chemical etching process","volume":"25","author":"Norek","year":"2017","journal-title":"J. Porous Mater."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1016\/j.jlumin.2010.12.027","article-title":"Photoluminescence properties of heat-treated porous alumina films formed in oxalic acid","volume":"131","author":"Vrublevsky","year":"2011","journal-title":"J. Lumin."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/0040-6090(83)90099-8","article-title":"Nature of the carboxylate species incorporated in anodic alumina films formed in oxalic acid solution","volume":"101","author":"Yamatomo","year":"1998","journal-title":"Thin Solid Film."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Patel, Y., Janusas, G., Palevicius, A., and Vilkauskas, A. (2020). Development of nanoporous AAO membrane for nano filtration using the acoustophoresis method. Sensors, 20.","DOI":"10.3390\/s20143833"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1590\/0104-6632.20170344s20160024","article-title":"Influence of the anodizing process variables on the acidic properties of anodic alumina films","volume":"34","author":"Boldrini","year":"2017","journal-title":"Braz. J. Chem. Eng."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1007\/s11090-019-10056-z","article-title":"Plasma polymer layers with primary amino groups for immobilization of nano- and microparticles","volume":"40","author":"Rao","year":"2019","journal-title":"Plasma Chem. Plasma Process."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"115674","DOI":"10.1016\/j.seppur.2019.115674","article-title":"Surface modification of alumina monolithic columns with 3-aminopropyltetraethoxysilane (APTES) for protein attachment","volume":"229","author":"Nayak","year":"2019","journal-title":"Sep. Purif. Technol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"338745","DOI":"10.1016\/j.aca.2021.338745","article-title":"Fluorescence \u201cturn-off\/turn-on\u201d biosensing of metal ions by gold nanoclusters, folic acid and reduced graphene oxide","volume":"1175","author":"Wong","year":"2021","journal-title":"Anal. Chim. Acta"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Zhou, Z., Shu, T., Sun, Y., Si, H., Peng, P., Su, L., and Zhang, X. (2021). Luminescent wearable biosensors based on gold nanocluster networks for \u201cturn-on\u201d detection of Uric acid, glucose and alcohol in sweat. Biosens. Bioelectron., 192.","DOI":"10.1016\/j.bios.2021.113530"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"3126","DOI":"10.1039\/C6AN00773B","article-title":"Functionalization of metal nanoclusters for biomedical applications","volume":"141","author":"Song","year":"2016","journal-title":"Analyst"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"8800","DOI":"10.1021\/nn202860s","article-title":"Synthesis of highly fluorescent metal (AG, AU, PT, and CU) nanoclusters by electrostatically induced reversible phase transfer","volume":"5","author":"Yuan","year":"2011","journal-title":"ACS Nano"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1039\/B920748A","article-title":"Highly selective and ultrasensitive detection ofHg2+ based on fluorescence quenching of Au nanoclusters by Hg2+\u2013Au+ interactions","volume":"46","author":"Xie","year":"2010","journal-title":"Chem. Commun."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"2568","DOI":"10.1021\/nl101225f","article-title":"On the Ligand\u2019s Role in the Fluorescence of Gold Nanoclusters","volume":"10","author":"Wu","year":"2010","journal-title":"Nano Lett."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/j.snb.2018.11.077","article-title":"Sensitive fluorescence sensor for point-of-care detection of trypsin using glutathione-stabilized gold nanoclusters","volume":"282","author":"Li","year":"2019","journal-title":"Sens. Actuators B Chem."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Bai, Y., Shu, T., Su, L., and Zhang, X. (2020). Fluorescent Gold nanoclusters for biosensor and bioimaging application. Crystals, 10.","DOI":"10.3390\/cryst10050357"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1161\/01.HYP.0000200042.64517.19","article-title":"Time Course of Maternal Plasma Volume and Hormonal Changes in Women with Preeclampsia or Fetal Growth Restriction","volume":"47","author":"Salas","year":"2006","journal-title":"Hypertension"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"e202301193","DOI":"10.1002\/anie.202301193","article-title":"Quantitative Point of Care Tests for Timely Diagnosis of Early-Onset Preeclampsia with High Sensitivity and Specificity","volume":"62","author":"Ghorbanpour","year":"2023","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1210\/clinem\/dgaa403","article-title":"Role of A Novel Angiogenesis FKBPL-CD44 Pathway in Preeclampsia Risk Stratification and Mesenchymal Stem Cell Treatment","volume":"106","author":"Todd","year":"2021","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"12279","DOI":"10.1039\/D1NR02461B","article-title":"A simplified point-of-care testing approach for preeclampsia blood biomarkers based on nanoscale field effect transistors","volume":"13","author":"Pham","year":"2021","journal-title":"Nanoscale"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"255","DOI":"10.2147\/IJWH.S368454","article-title":"Perspectives on the Use of Placental Growth Factor (PlGF) in the Prediction and Diagnosis of Pre-Eclampsia: Recent Insights and Future Steps","volume":"15","author":"Creswell","year":"2023","journal-title":"Int. J. Women\u2019s Health"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1056\/NEJMoa031884","article-title":"Circulating angiogenic factors and the risk of preeclampsia","volume":"350","author":"Levine","year":"2004","journal-title":"N. Engl. J. Med."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1021\/acssensors.2c02077","article-title":"Using electrochemical immunoassay in a novel microtiter plate to detect surface markers of preeclampsia on urinary extracellular vesicles","volume":"8","author":"Lee","year":"2022","journal-title":"ACS Sens."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Chaturvedi, N., Kunkel, A., Schechinger, M., Mabbott, S., Choudhury, M., and Cot\u00e9, G.L. (2023). Paper fluidic platform with dual-optical readouts to detect microRNA-20a (miR-20a) for preeclampsia. Optical Diagnostics and Sensing XXIII: Toward Point-of-Care Diagnostics, SPIE.","DOI":"10.1117\/12.2648423"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"e12584","DOI":"10.1111\/aji.12584","article-title":"Prospects of electrochemical immunosensors for early diagnosis of preeclampsia","volume":"77","author":"Suman","year":"2016","journal-title":"Am. J. Reprod. Immunol."}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/14\/12\/610\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:54:37Z","timestamp":1760115277000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/14\/12\/610"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,13]]},"references-count":79,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["bios14120610"],"URL":"https:\/\/doi.org\/10.3390\/bios14120610","relation":{},"ISSN":["2079-6374"],"issn-type":[{"value":"2079-6374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,13]]}}}