{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T12:53:13Z","timestamp":1785847993285,"version":"3.56.0"},"reference-count":49,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,12,22]],"date-time":"2024-12-22T00:00:00Z","timestamp":1734825600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Beijing Natural Science Foundation","award":["7232342"],"award-info":[{"award-number":["7232342"]}]},{"name":"Beijing Natural Science Foundation","award":["2242027"],"award-info":[{"award-number":["2242027"]}]},{"name":"Beijing Natural Science Foundation","award":["22474007"],"award-info":[{"award-number":["22474007"]}]},{"name":"Beijing Natural Science Foundation","award":["buctrc202415"],"award-info":[{"award-number":["buctrc202415"]}]},{"name":"Natural Science Foundation of China","award":["7232342"],"award-info":[{"award-number":["7232342"]}]},{"name":"Natural Science Foundation of China","award":["2242027"],"award-info":[{"award-number":["2242027"]}]},{"name":"Natural Science Foundation of China","award":["22474007"],"award-info":[{"award-number":["22474007"]}]},{"name":"Natural Science Foundation of China","award":["buctrc202415"],"award-info":[{"award-number":["buctrc202415"]}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["7232342"],"award-info":[{"award-number":["7232342"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2242027"],"award-info":[{"award-number":["2242027"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["22474007"],"award-info":[{"award-number":["22474007"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["buctrc202415"],"award-info":[{"award-number":["buctrc202415"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Peroxynitrite (ONOO\u2212) plays an important role in many physiological and pathological processes. Excessive ONOO\u2212 in cells leads to oxidative stress and inflammation. However, precise monitoring of ONOO\u2212 levels in specific organelles (e.g., mitochondria) is still lacking and urgently needed. Herein, we rationally designed a mitochondria-targeted ratiometric fluorescent probe, MOBDP-I, for imaging of ONOO\u2212 in the mitochondria of inflammatory cells and model mice. This probe, MOBDP-I, was synthesized by conjugating a BODIPY fluorophore to a mitochondria-targeting moiety\u2013indole-salt group by a carbon\u2013carbon double bond (C=C). In the presence of ONOO\u2212, the C=C bond between the BODIPY backbone and the indole-salt group was oxidized and broken, leading to an 18-fold enhancement of fluorescence at 510 nm, along with a significant fluorescence decrease at 596 nm. The ratiometric response property bestowed the probe with advantages in the precise quantification of ONOO\u2212 in cells, thus allowing estimation of the extent of inflammation in living cells and mouse models of rheumatoid arthritis, peritonitis, and brain inflammation. MOBDP-I could act as an effective molecular tool to study the relationship between ONOO\u2212 and the occurrence and development of inflammatory diseases.<\/jats:p>","DOI":"10.3390\/bios14120638","type":"journal-article","created":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T10:06:52Z","timestamp":1734948412000},"page":"638","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["BODIPY-Based Ratiometric Fluorescent Probe for Sensing Peroxynitrite in Inflammatory Cells and Tissues"],"prefix":"10.3390","volume":"14","author":[{"given":"Qian","family":"Wu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Chemical Resource Engineering, College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziwei","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemical Resource Engineering, College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemical Resource Engineering, College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulong","family":"Jin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemical Resource Engineering, College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuo","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemical Resource Engineering, College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.niox.2019.03.003","article-title":"The origins of nitric oxide and peroxynitrite research in Uruguay: 25 years of contributions to the biochemical and biomedical sciences","volume":"87","author":"Radi","year":"2019","journal-title":"Nitric Oxide"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"9746358","DOI":"10.1155\/2019\/9746358","article-title":"The Effect of Standardised Flower Extracts of Sorbus aucuparia L. on Proinflammatory Enzymes, Multiple Oxidants, and Oxidative\/Nitrative Damage of Human Plasma Components In Vitro","volume":"2019","author":"Olszewska","year":"2019","journal-title":"Oxid. 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