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Project","award":["122C195"],"award-info":[{"award-number":["122C195"]}]},{"name":"T\u00dcB\u0130TAK-1001 Scientific and Technological Research Project","award":["123S582"],"award-info":[{"award-number":["123S582"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Human monkeypox (Mpox) is a zoonotic disease caused by the Monkeypox virus (MPXV). As of 14 August 2024, the World Health Organization (WHO) has declared it a global health emergency. For Mpox, this was the second public health emergency of global significance in the past two years. MPXV belongs to the Poxviridae family and is phylogenetically and epidemically divided into two clades: the Congo Basin (Clade-I) and the West African (Clade-II) clades. Clade-I has been associated with more severe disease progression and higher mortality compared to Clade-II, and thus the differentiation between clades can play an important role in predicting disease prognosis. The LAMP technique has the advantages of not requiring thermal cycling and achieving higher amplification in a shorter time compared to qPCR. Different types of LAMP assays were developed in this study to benefit from these advantages. We report the development of LAMP-1 and LAMP-2 assays using the LAMP method to detect MPXV Clade-I and Clade-II, respectively. The LAMP-1 assay includes both fluorescence and visible colorimetric readout tests developed with sensitivities of 103 and 107 copies, respectively. For the LAMP-2 assay, a probe-based test utilizing the Novel R-Duplex DARQ probe was developed, offering fluorescence detection at a sensitivity of 103 copies. As a result, we successfully developed three highly specific molecular diagnostic tests that distinctly differentiate between MPXV clades, delivering essential tools for the precise diagnosis and effective control of Mpox.<\/jats:p>","DOI":"10.3390\/bios15010023","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T06:43:04Z","timestamp":1736145784000},"page":"23","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Development and Validation of LAMP Assays for Distinguishing MPXV Clades with Fluorescent and Colorimetric Readouts"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2020-9659","authenticated-orcid":false,"given":"Nazente","family":"Atceken","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences and Engineering, Ko\u00e7 University, 34450 Istanbul, Turkey"},{"name":"Ko\u00e7 University Translational Medicine Research Center (KUTTAM), Ko\u00e7 University, 34450 Istanbul, Turkey"},{"name":"School of Medicine, Ko\u00e7 University, 34450 Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7334-1736","authenticated-orcid":false,"given":"Sara","family":"Asghari Dilmani","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences and Engineering, Ko\u00e7 University, 34450 Istanbul, Turkey"},{"name":"Ko\u00e7 University Translational Medicine Research Center (KUTTAM), Ko\u00e7 University, 34450 Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2313-184X","authenticated-orcid":false,"given":"Ahmed Choukri","family":"Abdullah","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, Ko\u00e7 University, 34450 Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mutlu","family":"Sar\u0131kaya","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Pharmacy, Ankara University, 06560 Ankara, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Defne","family":"Yigci","sequence":"additional","affiliation":[{"name":"School of Medicine, Ko\u00e7 University, 34450 Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3574-1015","authenticated-orcid":false,"given":"Gozde","family":"Korkmaz","sequence":"additional","affiliation":[{"name":"Ko\u00e7 University Translational Medicine Research Center (KUTTAM), Ko\u00e7 University, 34450 Istanbul, Turkey"},{"name":"School of Medicine, Ko\u00e7 University, 34450 Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4604-217X","authenticated-orcid":false,"given":"Savas","family":"Tasoglu","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences and Engineering, Ko\u00e7 University, 34450 Istanbul, Turkey"},{"name":"Ko\u00e7 University Translational Medicine Research Center (KUTTAM), Ko\u00e7 University, 34450 Istanbul, Turkey"},{"name":"Department of Mechanical Engineering, Ko\u00e7 University, 34450 Istanbul, Turkey"},{"name":"Ko\u00e7 University & Is Bank Artificial Intelligence Center (KUIS AI), Ko\u00e7 University, 34450 Istanbul, Turkey"},{"name":"Ko\u00e7 University Ar\u00e7elik Research Center for Creative Industries (KUAR), Ko\u00e7 University, 34450 Istanbul, Turkey"},{"name":"Bo\u011fazi\u00e7i Institute of Biomedical Engineering, Bo\u011fazi\u00e7i University, 34684 Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1038\/s41579-022-00776-z","article-title":"Monkeypox virus: A neglected zoonotic pathogen spreads globally","volume":"20","author":"Alakunle","year":"2022","journal-title":"Nat. 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