{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:35:42Z","timestamp":1759970142575,"version":"build-2065373602"},"reference-count":49,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T00:00:00Z","timestamp":1736294400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Research Program of Chongqing Municipal Education Commission","award":["KJZD-M202400501","CYS23395"],"award-info":[{"award-number":["KJZD-M202400501","CYS23395"]}]},{"name":"Postgraduate Scientific Research and Innovation Project of Chongqing Municipal Education Commission","award":["KJZD-M202400501","CYS23395"],"award-info":[{"award-number":["KJZD-M202400501","CYS23395"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Sulfaquinoxaline (SQX) is widely utilized in aquaculture and animal husbandry due to its broad antimicrobial spectrum and low cost. However, it is difficult to degrade, and there are relevant residues in the aquatic environment, which could be harmful to both the ecological environment and human health. As a new recognition molecule, the aptamer can be recognized with SQX with high affinity and specificity, and the aptamer is no longer adsorbed to AuNPs after binding to SQX, which weakens the catalytic effect of AuNPs. Consequently, an aptasensor for the detection of SQX was successfully developed. This aptasensor exhibits a linear range of 40\u2013640 ng\/mL, with a detection limit of 36.95 ng\/mL, demonstrating both sensitivity and selectivity. The recoveries of this aptasensor in water samples ranged from 90 to 109.9%, which was quite in line with high-performance liquid chromatography. These findings suggest that the aptasensor is a valuable tool for detecting SQX in aqueous environmental samples.<\/jats:p>","DOI":"10.3390\/bios15010030","type":"journal-article","created":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T07:40:13Z","timestamp":1736322013000},"page":"30","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Label-Free Aptasensor for the Detection of Sulfaquinoxaline Using AuNPs and Aptamer in Water Environment"],"prefix":"10.3390","volume":"15","author":[{"given":"Zhaoyang","family":"Zhou","sequence":"first","affiliation":[{"name":"Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingyue","family":"Chen","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6750-9993","authenticated-orcid":false,"given":"Shuang","family":"Jiang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuoer","family":"Chen","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sixian","family":"Wang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yueyang","family":"Ren","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2390-5729","authenticated-orcid":false,"given":"Xiaodong","family":"Fan","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2771-7744","authenticated-orcid":false,"given":"Tao","family":"Le","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.bcp.2016.10.001","article-title":"History of antimicrobial drug discovery: Major classes and health impact","volume":"133","author":"Aminov","year":"2017","journal-title":"Biochem. Pharmacol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.agee.2018.01.026","article-title":"Veterinary antibiotics (VAs) contamination as a global agro-ecological issue: A critical view","volume":"257","author":"Kuppusamy","year":"2018","journal-title":"Agric. Ecosyst. Environ."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1016\/j.scitotenv.2018.09.315","article-title":"Photolysis of enrofloxacin, pefloxacin and sulfaquinoxaline in aqueous solution by UV\/H2O2, UV\/Fe(II), and UV\/H2O2\/Fe(II) and the toxicity of the final reaction solutions on zebrafish embryos","volume":"651","author":"Qiu","year":"2018","journal-title":"Sci. Total Environ."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Liu, T.-S., Wang, X.-Z., Liu, Y.-C., Zhao, C., Lin, H., and Zou, Y.-H. (2023). Using quantum chemistry theory to elucidate the mechanism for treating sulfonamide antibiotic wastewater by progressive freezing. J. Water Process Eng., 53.","DOI":"10.1016\/j.jwpe.2023.103891"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1007\/s00604-018-3079-9","article-title":"A Cu(II)-anchored unzipped covalent triazine framework with peroxidase-mimicking properties for molecular imprinting\u2013based electrochemiluminescent detection of sulfaquinoxaline","volume":"185","author":"Ma","year":"2018","journal-title":"Microchim. Acta"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Wang, Y., Gao, J., Duan, W., Zhang, W., Zhao, Y., and Liu, J. (2020). Inactivation of sulfonamide antibiotic resistant bacteria and control of intracellular antibiotic resistance transmission risk by sulfide-modified nanoscale zero-valent iron. J. Hazard. Mater., 400.","DOI":"10.1016\/j.jhazmat.2020.123226"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2833","DOI":"10.1007\/s12274-017-1490-x","article-title":"Gold immunochromatographic sensor for the rapid detection of twenty-six sulfonamides in foods","volume":"10","author":"Chen","year":"2017","journal-title":"Nano Res."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Chen, Q., Pan, Y., Sun, C., Wang, Z., Wu, Y., and Fu, F. (2023). A multicolor immunosensor for the visual detection of six sulfonamides based on manganese dioxide nanosheet-mediated etching of gold nanobipyramids. Talanta, 258.","DOI":"10.1016\/j.talanta.2023.124449"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.chroma.2018.12.003","article-title":"Tolerance intervals modeling for design space of a salt assisted liquid-liquid microextraction of trimethoprim and six common sulfonamide antibiotics in environmental water samples","volume":"1586","author":"Mokhtar","year":"2018","journal-title":"J. Chromatogr. A"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.talanta.2014.02.010","article-title":"Porous molecularly imprinted monolithic capillary column for on-line extraction coupled to high-performance liquid chromatography for trace analysis of antimicrobials in food samples","volume":"123","author":"Zhang","year":"2014","journal-title":"Talanta"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4172\/2153-2435.1000168","article-title":"Validated HPLC Method to Simultaneously Determine Amprolium Hydrochloride, Sulfaquinoxaline Sodium and Vitamin K3 in A.S.K Powder on ZIC-HILIC Column","volume":"3","author":"Ghanem","year":"2012","journal-title":"Pharm. Anal. Acta"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.jchromb.2014.01.008","article-title":"Determination of 26 veterinary antibiotics residues in water matrices by lyophilization in combination with LC\u2013MS\/MS","volume":"949\u2013950","author":"Hu","year":"2014","journal-title":"J. Chromatogr. B"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Li, T., Wang, C., Xu, Z., and Chakraborty, A. (2020). A coupled method of on-line solid phase extraction with the UHPLC\u2013MS\/MS for detection of sulfonamides antibiotics residues in aquaculture. Chemosphere, 254.","DOI":"10.1016\/j.chemosphere.2020.126765"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.chroma.2016.05.034","article-title":"On-line solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry as a powerful technique for the determination of sulfonamide residues in soils","volume":"1452","author":"Tetzner","year":"2016","journal-title":"J. Chromatogr. A"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1002\/elps.201500448","article-title":"Field-enhanced sample injection micelle-to-solvent stacking capillary zone electrophoresis-electrospray ionization mass spectrometry of antibiotics in seawater after solid-phase extraction","volume":"37","author":"Wuethrich","year":"2016","journal-title":"Electrophoresis"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1016\/j.talanta.2008.04.062","article-title":"Use of experimental design and effective mobility calculations to develop a method for the determination of antimicrobials by capillary electrophoresis","volume":"76","author":"Mamani","year":"2008","journal-title":"Talanta"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"7550","DOI":"10.1021\/ac101020y","article-title":"Utilizing Three Monoclonal Antibodies in the Development of an Immunochromatographic Assay for Simultaneous Detection of Sulfamethazine, Sulfadiazine, and Sulfaquinoxaline Residues in Egg and Chicken Muscle","volume":"82","author":"Guo","year":"2010","journal-title":"Anal. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1038\/346818a0","article-title":"In vitro selection of RNA molecules that bind specific ligands","volume":"346","author":"Ellington","year":"1990","journal-title":"Nature"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1126\/science.2200121","article-title":"Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase","volume":"249","author":"Tuerk","year":"1990","journal-title":"Science"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Chuesiang, P., Ryu, V., Siripatrawan, U., He, L., and McLandsborough, L. (2021). Aptamer-based surface enhanced Raman spectroscopy (SERS) for the rapid detection of Salmonella Enteritidis contaminated in ground beef. LWT\u2014Food Sci. Technol., 150.","DOI":"10.1016\/j.lwt.2021.111937"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1007\/s00604-019-3261-8","article-title":"Fluorometric determination of lipopolysaccharides via changes of the graphene oxide-enhanced fluorescence polarization caused by truncated aptamers","volume":"186","author":"Ye","year":"2019","journal-title":"Microchim. Acta"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1021\/acscentsci.2c01263","article-title":"Robust Covalent Aptamer Strategy Enables Sensitive Detection and Enhanced Inhibition of SARS-CoV-2 Proteins","volume":"9","author":"Wang","year":"2023","journal-title":"ACS Cent. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Sabrowski, W., Dreymann, N., M\u00f6ller, A., Czepluch, D., Albani, P.P., Theodoridis, D., and Menger, M.M. (2022). The use of high-affinity polyhistidine binders as masking probes for the selection of an NDM-1 specific aptamer. Sci. Rep., 12.","DOI":"10.1038\/s41598-022-12062-2"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2552","DOI":"10.1021\/acsnano.3c09576","article-title":"Aptamer Renaissance for Neurochemical Biosensing","volume":"18","author":"Stuber","year":"2024","journal-title":"ACS Nano"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2544","DOI":"10.1007\/s00259-022-05782-0","article-title":"Advances in aptamer-based nuclear imaging","volume":"49","author":"Song","year":"2022","journal-title":"Eur. J. Nucl. Med. Mol. Imaging"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5095","DOI":"10.1021\/acs.analchem.3c00005","article-title":"Development of Uveal Melanoma-Specific Aptamer for Potential Biomarker Discovery and Targeted Drug Delivery","volume":"95","author":"Pan","year":"2023","journal-title":"Anal. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Zheng, X., Jiang, S., Ren, Y., Wang, S., Xie, Y., and Le, T. (2024). High-efficient selection of aptamers by magnetic cross-linking precipitation and development of aptasensor for 1-aminohydantoin detection. LWT\u2014Food Sci. Technol., 199.","DOI":"10.1016\/j.lwt.2024.116128"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Tang, J., Zheng, X., Jiang, S., Cao, M., Wang, S., Zhou, Z., Nie, X., Fang, Y., and Le, T. (2022). Dual fluorescent aptasensor for simultanous and quantitative detection of sulfadimethoxine and oxytetracycin residues in animal-derived foods tissues based on mesoporous silica. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.1077893"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Zhang, L., Wu, S., Liu, J., Ping, M., Yang, W., and Fu, F. (2024). Isolation of aptamers with excellent cross-reactivity and specificity to sulfonamides towards a ratiometric fluorescent aptasensor for the detection of nine sulfonamides in seafood. Talanta, 277.","DOI":"10.1016\/j.talanta.2024.126380"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Li, S., He, B., Liang, Y., Wang, J., Jiao, Q., Liu, Y., Guo, R., Wei, M., and Jin, H. (2021). Sensitive electrochemical aptasensor for determination of sulfaquinoxaline based on AuPd NPs@UiO-66-NH2\/CoSe2 and RecJf exonuclease-assisted signal amplification. Anal. Chim. Acta, 1182.","DOI":"10.1016\/j.aca.2021.338948"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Dai, J., Li, J., Jiao, Y., Yang, X., Yang, D., Zhong, Z., Li, H., and Yang, Y. (2024). Colorimetric-SERS dual-mode aptasensor for Staphylococcus aureus based on MnO2@AuNPs oxidase-like activity. Food Chem., 456.","DOI":"10.1016\/j.foodchem.2024.139955"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Yu, C., Huang, Z., Ping, T., Su, H., Yang, Q., and Wu, W. (2023). Dual-mode aptasensors based on AuNPs and Ag@Au NPs for simultaneous detection of foodborne pathogens. LWT\u2014Food Sci. Technol., 184.","DOI":"10.1016\/j.lwt.2023.115007"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2136","DOI":"10.1007\/s12161-021-02029-w","article-title":"Research Progress of Optical Aptasensors Based on AuNPs in Food Safety","volume":"14","author":"Song","year":"2021","journal-title":"Food Anal. Methods"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Yang, Y., Yin, Y., Li, X., Wang, S., and Dong, Y. (2020). Development of a chimeric aptamer and an AuNPs aptasensor for highly sensitive and specific identification of Aflatoxin B1. Sens. Actuators B Chem., 319.","DOI":"10.1016\/j.snb.2020.128250"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Wang, S., Zhao, Y., Ma, R., Wang, W., Zhang, L., Li, J., Sun, J., and Mao, X. (2022). Aptasensing a class of small molecules based on split aptamers and hybridization chain reaction-assisted AuNPs nanozyme. Food Chem., 401.","DOI":"10.1016\/j.foodchem.2022.134053"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Tavakoli, P., Taghdisi, S.M., Maghami, P., and Abnous, K. (2021). A Novel Aptasensor for Colorimetric Monitoring of Tobramycin: Strategy of Enzyme-Like Activity of AuNPs Controlled by Three-Way Junction DNA Pockets. Spectrochim. Acta Part A Mol. Biomol. Spectrosc., 267.","DOI":"10.1016\/j.saa.2021.120626"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Wu, Y.-Y., Huang, P., and Wu, F.-Y. (2019). A label-free colorimetric aptasensor based on controllable aggregation of AuNPs for the detection of multiplex antibiotics. Food Chem., 304.","DOI":"10.1016\/j.foodchem.2019.125377"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1007\/s12161-020-01869-2","article-title":"Selection and Application of DNA Aptamers Against Sulfaquinoxaline Assisted by Graphene Oxide\u2013Based SELEX","volume":"14","author":"Shi","year":"2020","journal-title":"Food Anal. Methods"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Chen, X., Yang, L., Tang, J., Wen, X., Zheng, X., Chen, L., Li, J., Xie, Y., and Le, T. (2022). An AuNPs-Based Fluorescent Sensor with Truncated Aptamer for Detection of Sulfaquinoxaline in Water. Biosensors, 12.","DOI":"10.3390\/bios12070513"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"6187","DOI":"10.1039\/D2TB01115H","article-title":"A label-free dual-modal aptasensor for colorimetric and fluorescent detection of sulfadiazine","volume":"10","author":"Yang","year":"2022","journal-title":"J. Mater. Chem. B"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Yang, Y., Han, Y., Sun, Q., Cheng, J., Yue, C., Liu, Y., Song, J., Jin, W., Ding, X., and de la Fuente, J.M. (2021). Au-siRNA@ aptamer nanocages as a high-efficiency drug and gene delivery system for targeted lung cancer therapy. J. Nanobiotechnol., 19.","DOI":"10.1186\/s12951-020-00759-3"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3581","DOI":"10.1039\/C5AN00107B","article-title":"Aptamer-based colorimetric biosensing of abrin using catalytic gold nanoparticles\u2020","volume":"140","author":"Hu","year":"2015","journal-title":"Analyst"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1021\/acs.analchem.5b03926","article-title":"Multiplexed Activity of perAuxidase: DNA-Capped AuNPs Act as Adjustable Peroxidase","volume":"88","author":"Hizir","year":"2015","journal-title":"Anal. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Zhang, Z., Tian, Y., Huang, P., and Wu, F.-Y. (2019). Using target-specific aptamers to enhance the peroxidase-like activity of gold nanoclusters for colorimetric detection of tetracycline antibiotics. Talanta, 208.","DOI":"10.1016\/j.talanta.2019.120342"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/09540105.2010.514895","article-title":"Monoclonal antibody against sulfaquinoxaline and quantitative analysis in chicken tissues by competitive indirect ELISA and lateral flow immunoassay","volume":"22","author":"Ngom","year":"2011","journal-title":"Food Agric. Immunol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Li, C., Luo, X., Li, Y., Yang, H., Liang, X., Wen, K., Cao, Y., Li, C., Wang, W., and Shi, W. (2019). A Class-Selective Immunoassay for Sulfonamides Residue Detection in Milk Using a Superior Polyclonal Antibody with Broad Specificity and Highly Uniform Affinity. Molecules, 24.","DOI":"10.3390\/molecules24030443"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3908","DOI":"10.1021\/acs.jafc.6b01497","article-title":"Upconversion Nanoparticles and Monodispersed Magnetic Polystyrene Microsphere Based Fluorescence Immunoassay for the Detection of Sulfaquinoxaline in Animal-Derived Foods","volume":"64","author":"Hu","year":"2016","journal-title":"J. Agric. Food Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1264","DOI":"10.1080\/19440049.2013.801084","article-title":"Simultaneous detection of sulfamethazine and sulfaquinoxaline using a dual-label time-resolved fluorescence immunoassay","volume":"30","author":"Le","year":"2013","journal-title":"Food Addit. Contam. Part A"},{"key":"ref_49","first-page":"504","article-title":"Development of a novel carbon paste sensor for determination of micromolar amounts of sulfaquinoxaline in pharmaceutical and biological samples","volume":"58","author":"Soleymanpour","year":"2015","journal-title":"Biomater. Adv."}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/15\/1\/30\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:24:59Z","timestamp":1759919099000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/15\/1\/30"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,8]]},"references-count":49,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,1]]}},"alternative-id":["bios15010030"],"URL":"https:\/\/doi.org\/10.3390\/bios15010030","relation":{},"ISSN":["2079-6374"],"issn-type":[{"type":"electronic","value":"2079-6374"}],"subject":[],"published":{"date-parts":[[2025,1,8]]}}}